Heat-transfer-based detection of SNPs in the PAH gene of PKU patients

Bon, Natalie Vanden; Grinsven, Bart van; Murib, Mohammed Sharif; Yeap, Weng Siang; Haenen, Ken; Ceuninck, W. De; Wagner, Patrick; Ameloot, Marcel; Vermeeren, Veronique; Michiels, Luc

doi:10.2147/ijn.s58692

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…Chin et al (2014a) proposed that nanostructured DNA biosensors could detect base mutations. To speed up and simplify mutation screening in genes, Vanden Bon et al (2014) developed a method based on the change in heat transfer resistance upon thermal denaturation of double-stranded DNA on nanocrystalline diamond to detect the mutation in entire exons of the phenylalanine hydroxylase gene in phenylketonuria patients. To determine whether a fetus had a genetic defect, amniotic fluid technology, an expensive and possibly harmful diagnosis, normally was used.…”

Section: Unique Physical and Chemical Properties Of Nanomaterialsmentioning

confidence: 99%

Potential applications and human biosafety of nanomaterials used in nanomedicine

Bowman

et al. 2017

J of Applied Toxicology

131

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With the rapid development of nanotechnology, potential applications of nanomaterials in medicine have been widely researched in recent years. Nanomaterials themselves can be used as image agents or therapeutic drugs, and for drug and gene delivery, biological devices, nanoelectronic biosensors or molecular nanotechnology. As the composition, morphology, chemical properties, implant sites as well as potential applications become more and more complex, human biosafety of nanomaterials for clinical use has become a major concern. If nanoparticles accumulate in the human body or interact with the body molecules or chemical components, health risks may also occur. Accordingly, the unique chemical and physical properties, potential applications in medical fields, as well as human biosafety in clinical trials are reviewed in this study. Finally, this article tries to give some suggestions for future work in nanomedicine research.

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Section: Unique Physical and Chemical Properties Of Nanomaterialsmentioning

confidence: 99%

Potential applications and human biosafety of nanomaterials used in nanomedicine

Bowman

et al. 2017

J of Applied Toxicology

131

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“…The recently developed heat‐transfer method (HTM) has shown to be a versatile read‐out platform in biosensors . HTM is a fast, label‐free, and sensitive technique that requires a minimum of instrumentation and can be used “on‐the‐bench.” HTM can be used for a wide range of applications including DNA mutation analysis , quantification of DNA sequences of interest , neurotransmitter detection , and phase transition analysis in lipid vesicle layers . Additionally, the platform can be used for the specific detection of human cells in phosphate buffered saline (PBS) solution by combining HTM with SIPs .…”

Section: Introductionmentioning

confidence: 99%

Improving the sensitivity of the heat‐transfer method (HTM) for cancer cell detection with optimized sensor chips

Eersels

Grinsven

Vandenryt

et al. 2015

Physica Status Solidi (a)

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Phone: þ32 (011) 26 88 93, Fax: þ32 (011) 26 88 99 † K. Eersels and B. van Grinsven contributed equally to this work.In this article, we increased the sensitivity of the heat-transfer method (HTM) for the detection of breast cancer cells (ZR-75-1 cells, see figure) in phosphate buffered saline (PBS). The effect of small technological changes on the limit of detection (LoD) of the methodology was examined. To this extent, polished aluminum substrates with a mirror finish were used, replacing the unpolished chips used in previous studies. These chips were coated with a polyurethane layer and imprinted for the target cell type, creating a so-called surface imprintedpolymer (SIP). Binding of target cells to the SIP resulted in an increase of the thermal resistance at the solid-liquid interface under study. Background thermal resistance measurements were performed with polished and unpolished aluminum substrates. In addition, the effect of using silver paste as thermal coupling between the aluminum chip and the copper heat provider was analyzed. The results of these experiments reveal that optimal thermal contact is achieved when directly coupling the copper heat provider to the polished side of the aluminum substrate as evidenced by a decrease in the baseline thermal resistance. In addition, noise levels on the heat-transfer resistance (R th ) signal decreased by a factor in the optimal configuration. Dose-response curves were obtained using the optimized methodology and were compared with results obtained with the original substrates. These quantitative experiments demonstrated an improvement of the LoD by approximately thirty percent.ZR-75-1 cells applied onto a home-made rubber stamp.

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“…The focus of this manuscript is on hybridization techniques which are appealing due to simplicity: a nucleic acid probe is designed to hybridize to the perfectly matching target molecule with a high efficiency while having a low efficiency for targets 10 containing sequence variations. The principle is open to many technical implementations and miniaturization for use in biosensor devices [1,2,3,4,5,6]. However, hybridization has challenges on the side of probe design and dynamic range due to hybridization stringency conditions [7,8,9] which are not easy to optimize especially when parallelization is aimed for.…”

Section: Introductionmentioning

confidence: 99%

Dynamic range extension of hybridization sensors

Hadiwikarta

Carlon

Hooyberghs

2015

Biosensors and Bioelectronics

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In hybridization based nucleic acid sensors the stringency of hybridization poses a challenge to design and experiment. For a given set of experimental parameters the affinity window of probe-target interaction is always limited and vice versa for a given probe set design, changes in experimental conditions can easily bring some measurements out of detection range. In this paper we introduce and apply a strategy to extend this dynamic range for affinity sensors, sensors which measure the amount of hybridized molecules after equilibrium is reached. The method relies on concepts of additivity of nucleic acids hybridization free energies and on equilibrium isotherms. It consists in combining the measurements from probes with different length, by appropriately rescaling the measured signals. We test the validity of the approach on experiments and show that by combining probes with hybridizing regions of length 21, 23 and 25 nucleotides we manage to extend the dynamic range of the intensity signals by a factor 25. The presented concept is easy to extend, platform free and applies to any hybridization based affinity sensor.

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Heat-transfer-based detection of SNPs in the PAH gene of PKU patients

Cited by 9 publications

References 29 publications

Potential applications and human biosafety of nanomaterials used in nanomedicine

Potential applications and human biosafety of nanomaterials used in nanomedicine

Improving the sensitivity of the heat‐transfer method (HTM) for cancer cell detection with optimized sensor chips

Dynamic range extension of hybridization sensors

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