Heat shock response in the thermophilic fungus Rhizomucor miehei

Yanutsevich, E. A.; Memorskaya, A. S.; Groza, N. V.; Кочкина, Г. А.; Терешина, В. М.

doi:10.1134/s0026261714050282

Cited by 13 publications

(14 citation statements)

References 19 publications

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“…However, the degree of unsaturation of the fatty acids in the phospholipids did not decrease. We detected a high level of trehalose in the cells of fungi during growth under optimum temperature, but in response to HS, there was a sharp reduction in the amount of this disaccharide (Yanutsevich et al, 2014). These results are contradictory to other observations (Oberson et al, 1999;Maheshwari et al, 2000); however, they were from only one species of fungi.…”

Section: Introductioncontrasting

confidence: 98%

“…But 3 h HS resulted, as in R. tauricus, in a decrease of the proportions of PC and PE, and in a detectable increase in PA and St. In the minor lipids, a decrease in PI and LPE and an increase in SL were observed at 3 h. Therefore, the response of both fungi to HS included an increase in the proportion of two components, PA and St, which was in agreement with our previous data from R. miehei (Yanutsevich et al, 2014).…”

Section: Fungal Membrane Lipids Under Optimum Temperature Conditions supporting

confidence: 91%

“…The amount of glucose did not change significantly. The results obtained are in agreement with our previous observations made in experiments with another thermophilic fungi, R. miehei (Yanutsevich et al, 2014), but they do not agree with data showing a HS-induced trehalose synthesis increase in thermophilic C. coprophilum (Jepsen & Jensen, 2004).…”

Section: Soluble Cytosol Carbohydrates Of the Fungi Under Optimum Temsupporting

confidence: 64%

“…Analyzing the changes in the membrane lipid components, we can conclude that, contrary to the 'homeoviscous adaptation' hypothesis (Sinensky, 1974), no decrease in the degree of unsaturation of the major membrane phospholipid acyl chains occurred under HS conditions. Moreover, our earlier studies demonstrated that in R. miehei, the degree of unsaturation of PC and PE fatty acids markedly increased (Yanutsevich et al, 2014). This indicates that changes in unsaturation are species-specific and not directly linked to the HS response.…”

Section: Discussionmentioning

confidence: 86%

“…with R. meihei demonstrated a decrease in trehalose content after 3 h of HS (Yanutsevich et al, 2014). We therefore analyzed the soluble cytosolic carbohydrate content in dynamics in R. tauricus and M. thermophila and under optimal and HS conditions.…”

Section: Soluble Cytosol Carbohydrates Of the Fungi Under Optimum Temmentioning

confidence: 99%

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Heat shock response of thermophilic fungi: membrane lipids and soluble carbohydrates under elevated temperatures

et al. 2016

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The heat shock (HS) response is an adaptation of organisms to elevated temperature. It includes substantial changes in the composition of cellular membranes, proteins and soluble carbohydrates. To protect the cellular macromolecules, thermophilic organisms have evolved mechanisms of persistent thermotolerance. Many of those mechanisms are common for thermotolerance and the HS response. However, it remains unknown whether thermophilic species respond to HS by further elevating concentrations of protective components. We investigated the composition of the soluble cytosol carbohydrates and membrane lipids of the thermophilic fungi Rhizomucor tauricus and Myceliophthora thermophilaat optimum temperature conditions (41-43 С), and under HS (51-53 С). At optimum temperatures, the membrane lipid composition was characterized by a high proportion of phosphatidic acids (PA) (20-35 % of the total), which were the main components of the membrane lipids, together with phosphatidylcholines (PC), phosphatidylethanolamines (PE) and sterols (St). In response to HS, the proportion of PA and St increased, and the amount of PC and PE decreased. No decrease in the degree of fatty acid desaturation in the major phospholipids under HS was detected. The mycelium of all fungi at optimum temperatures contained high levels of trehalose (8-10 %, w/w; 60-95 % of the total carbohydrates), which is a hallmark of thermophilia. In contrast to mesophilic fungi, heat exposure decreased the trehalose level and the fungi did not acquire thermotolerance to lethal HS, indicating that trehalose plays a key role in this process. This pattern of changes appears to be conserved in the studied filamentous thermophilic fungi.

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Section: Introductioncontrasting

confidence: 98%

Section: Fungal Membrane Lipids Under Optimum Temperature Conditions supporting

confidence: 91%

Section: Soluble Cytosol Carbohydrates Of the Fungi Under Optimum Temsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 86%

Section: Soluble Cytosol Carbohydrates Of the Fungi Under Optimum Temmentioning

confidence: 99%

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Heat shock response of thermophilic fungi: membrane lipids and soluble carbohydrates under elevated temperatures

et al. 2016

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Lipid and Metabolite Profiling of Serpula lacrymans Under Freezing Stress

et al. 2021

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Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH

et al. 2017

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Alkaliphily, the ability of an organism to thrive optimally at high ambient pH, has been well-documented in several lineages: archaea, bacteria and fungi. The molecular mechanics of such adaptation has been extensively addressed in alkaliphilic bacteria and alkalitolerant fungi. In this study, we consider an additional property that may have enabled fungi to prosper at alkaline pH: altered contents of membrane lipids and cytoprotectant molecules. In the alkaliphilic Sodiomyces tronii, we showed that at its optimal growth pH 9.2, the fungus accumulates abundant cytosolic trehalose (4-10% dry weight) and phosphatidic acids in the membrane lipids, properties not normally observed in neutrophilic species. At a very high pH 10.2, the major carbohydrate, glucose, was rapidly substituted by mannitol and arabitol. Conversely, lowering the pH to 5.4-7.0 had major implications both on the content of carbohydrates and membrane lipids. It was shown that trehalose dominated at pH 5.4. Fractions of sphingolipids and sterols of plasma membranes rapidly elevated possibly indicating the formation of membrane structures called rafts. Overall, our results reveals complex dynamics of the contents of membrane lipids and cytoplasmic sugars in alkaliphilic S. tronii, suggesting their adaptive functionality against pH stress.

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Heat shock response in the thermophilic fungus Rhizomucor miehei

Cited by 13 publications

References 19 publications

Heat shock response of thermophilic fungi: membrane lipids and soluble carbohydrates under elevated temperatures

Heat shock response of thermophilic fungi: membrane lipids and soluble carbohydrates under elevated temperatures

Lipid and Metabolite Profiling of Serpula lacrymans Under Freezing Stress

Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH

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