Lipid and Metabolite Profiling of Serpula lacrymans Under Freezing Stress

Senik, Svetlana V.; Kolker, Tatiana L.; Котлова, Е. Р.; Vlasov, Dmitry Yu.; Шаварда, А. Л.; Puzanskiy, Roman; Psurtseva, Nadezhda V.

doi:10.1007/s00284-021-02349-4

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…Soluble carbohydrates increased in Ea-7D, predominantly in the class of damaged seeds. Water-soluble metabolites such as mannitol, glycerol, sugar alcohols and some amino and organic acids were found to be protective compounds under freezing stress [85]. The freezing-associated mortality of germinated but nonemergent seedlings is common in nature because of prewinter germination [86].…”

Section: Osmoprotectantsmentioning

confidence: 99%

Deterioration in the Quality of Recalcitrant Quercus robur Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study

et al. 2022

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Pedunculate oak (Quercus robur L.) is an economically important forest-forming species in Poland that produces seeds that are sensitive to desiccation; therefore, short-lived seeds are classified as recalcitrant. Such seeds display active metabolism throughout storage. Acorns stored under controlled conditions (moisture content of 40%, temperature −3 °C) maintain viability for up to 1.5–2 years. Meanwhile, oaks only produce large numbers of seeds every few years during so-called mast years. This results in a scarcity of good-quality seeds for continuous nursery production and restoration. The recalcitrant storage behavior and the requirements of foresters make it necessary to develop a new protocol for longer acorn storage at lower temperatures. Two storage temperatures were tested: −3 °C (currently used in forest practice) and −7 °C. Our results showed that acorns stored for six months exhibited deterioration and reduced germination capacity, as well as reduced seedling performance, particularly when acorns were stored at −7 °C. To elucidate the decrease in quality during storage, an untargeted metabolomics study was performed for the first time and supported with the analysis of carbohydrates and percentages of carbon (C) and nitrogen (N). Embryonic axes were characterized by a lower C:N ratio and higher hydration. A total of 1985 metabolites were detected, and 303 were successfully identified and quantified, revealing 44 known metabolites that displayed significantly up- or downregulated abundance. We demonstrated for the first time that the significant deterioration of seed germination potential, particularly in seeds stored at −7 °C, was accompanied by an increased abundance of phenolic compounds and carbohydrates but also amino acids and phosphorylated monosaccharides, particularly in the embryonic axes. The increased abundance of defense-related metabolites (1,2,4-Benzenetriol; BTO), products of ascorbic acid degradation (threonic and isothreonic acid), as well as antifreezing compounds (sugar alcohols, predominantly threitol), was reported in seed stored at −7 °C. We hypothesize that seed deterioration was caused by freezing stress experienced during six months of storage at −7 °C, a decline in antioxidative potential and the unsuccessful rerouting of the energy-production pathways. Additionally, our data are a good example of the application of high-throughput metabolomic tools in forest management.

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Section: Osmoprotectantsmentioning

confidence: 99%

Deterioration in the Quality of Recalcitrant Quercus robur Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study

et al. 2022

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“…An increase in PC concentration has been demonstrated under stress conditions. The PC content of the mycelium of the domestic fungus Serpula lacrymans increased under heat shock and freeze–thaw effects 24 . Even during periods of phosphate starvation, PC accumulation may continue or intensify when glycerophospholipids biosynthesis processes are drastically reduced 25 .…”

Section: Discussionmentioning

confidence: 99%

“…The PC content of the mycelium of the domestic fungus Serpula lacrymans increased under heat shock and freezethaw effects. 24 Even during periods of phosphate starvation, PC accumulation may continue or intensify when glycerophospholipids biosynthesis processes are drastically reduced. 25 Oxathiapiprolin treatment caused a significant increase in PC content, but had no effect on PE content of P. sojae.…”

Section: Discussionmentioning

confidence: 99%

Untargeted lipidomics reveals lipid metabolism disorders induced by oxathiapiprolin in Phytophthora sojae

Liu

Chen

et al. 2023

Pest Management Science

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Background: Oxathiapiprolin, an oxysterol-binding protein inhibitor (OSBPI), shows unexceptionable inhibitory activity against plant pathogenic oomycetes. FRAC (Fungicide Resistance Action Committee) classifies it into the mode of action group F9 (lipid homeostasis and transfer/storage), but very little is known about the lipid metabolism of oomycete pathogens when subjected to oxathiapiprolin.Results: In this study, seven lipid categories and 1435 lipid molecules were identified in Phytophthora sojae, among which glycerolipids, glycerophospholipids, and sphingolipids account for 30.10%, 50.59%, and 7.28%, respectively. These lipids were categorized into 31 subclasses, which varied to different extents when treated with oxathiapiprolin. A total of 11 lipid subclasses showed significant changes. Among them, 10 lipid subclasses, lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylcholine (PC), phosphatidylserine (PS), ceramide (Cer), triglyceride (TG), (o-acyl)-1-hydroxy fatty acid, diglycosylceramide, sphingoshine (So), and sitosterol ester, were significantly up-regulated, while digalactosyldiacylglycerol was the only lipid that was significantly down-regulated by a factor of almost three. These lipid molecules were further analyzed at the lipid species level. A total of 542 species were significantly altered when treated with oxathiapiprolin, including 212 glycerolipids [186 TG and 26 diglycerides (DG)], 167 glycerophospholipids (38 PC, 15 LPC, 19 LPE, seven PS, etc.), 156 sphingolipids (146 Cer, four So, etc.), and some other lipid molecules. Finally, from the orthogonal partial least-squares discrimination analysis model, variable importance for the projection score analysis showed that Cer, TG, and some glycerophospholipids contribute to the metabolic disorder when subjected to oxathiapiprolin. Conclusion: Glycerolipids, glycerophospholipids, and sphingolipids in P. sojae undergo significant changes with oxathiapiprolin treatment. These results provided valuable information for further understanding the function of the target protein and the mode of action of OSBPIs in oomycetes.

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“…For heterotrophic cells, this is the main way to obtain energy. In the profiles of the metabolites of plant cell cultures, various carbonic acids including intermediates of glycolysis and TCA are detected [ 157 , 158 , 159 , 160 ], possibly due to the accumulation of various carboxylic acids in the central vacuole [ 161 , 162 , 163 ]. Nevertheless, plant cells are significantly limited in their ability to assimilate carboxylic acid as the only carbon source.…”

Section: Organic Substratesmentioning

confidence: 99%

Plant Heterotrophic Cultures: No Food, No Growth

Puzanskiy,

Romanyuk,

Kirpichnikova

et al. 2024

Plants

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Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are Nicotiana tabacum and Arabidopsis thaliana. Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the heterotrophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as glycerol, carbonic acids, starch, and mannitol have the ability to support growth occasionally, or in combination with another substrate. Culture growth is accompanied by processes of specialization, such as elongation growth. This determines the pattern of the carbon budget. Culture ageing is closely linked to substrate depletion, changes in medium composition, and cell physiological rearrangements. A lack of substrate leads to starvation, which results in a decrease in physiological activity and the mobilization of resources, and finally in the loss of viability. The cause of the instability of cultivated cells may be the non-optimal metabolism under cultural conditions or the insufficiency of internal regulation.

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Lipid and Metabolite Profiling of Serpula lacrymans Under Freezing Stress

Cited by 5 publications

References 28 publications

Deterioration in the Quality of Recalcitrant Quercus robur Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study

Deterioration in the Quality of Recalcitrant Quercus robur Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study

Untargeted lipidomics reveals lipid metabolism disorders induced by oxathiapiprolin in Phytophthora sojae

Plant Heterotrophic Cultures: No Food, No Growth

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