Heat shock proteins and parasitic diseases: Part 1: Helminths

Abaza, Sherif

doi:10.4103/1687-7942.149556

Cited by 9 publications

(7 citation statements)

References 8 publications

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“…Various functions of HSPs that may be used to control bacterial infections have been studied (Bolhassani and Agi, 2019). Studies have shown that high expression of HSP90 may lead to the secretion of inflammatory cytokines in the body (Abaza, 2014). Studies have shown that some HSPs are effective inducers of innate and adaptive immunity.…”

Section: Discussionmentioning

confidence: 99%

Effects of Multispecies Probiotic on Intestinal Microbiota and Mucosal Barrier Function of Neonatal Calves Infected With E. coli K99

Nie

Luo³

et al. 2022

Front. Microbiol.

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Altered gut microbiota are implicated in inflammatory neonatal calf diarrhea caused by E. coli K99. Beneficial probiotics are used to modulate gut microbiota. However, factors that mediate host-microbe interactions remain unclear. We evaluated the effects of a combination of multispecies probiotics (MSP) on growth, intestinal epithelial development, intestinal immune function and microbiota of neonatal calves infected with E. coli K99. Twelve newborn calves were randomly assigned as follows: C (control, without MSP); D (E. coli O78:K99 + gentamycin); and P (E. coli O78:K99 + supplemental MSP). All groups were studied for 21 d. MSP supplementation significantly (i) changed fungal Chao1 and Shannon indices of the intestine compared with group D; (ii) reduced the relative abundance of Bacteroides and Actinobacteria, while increasing Bifidobacteria, Ascomycetes, and Saccharomyces, compared with groups C and D; (iii) improved duodenal and jejunal mucosal SIgA and total Short Chain Fatty Acids (SCFA) concentrations compared with group D; (iv) increased relative ZO-1 and occludin mRNA expression in jejunal mucosa compared with group D; and (v) enhanced intestinal energy metabolism and defense mechanisms of calves by reducing HSP90 expression in E. coli K99, thereby alleviating the inflammatory response and promoting recovery of mucosal function. Our research may provide direct theoretical support for future applications of MSP in ruminant production.

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Section: Discussionmentioning

confidence: 99%

Effects of Multispecies Probiotic on Intestinal Microbiota and Mucosal Barrier Function of Neonatal Calves Infected With E. coli K99

Nie

Luo³

et al. 2022

Front. Microbiol.

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“…Heat shock proteins are known to be molecular chaperones and are classified into different families according to their molecular weights. They primarily involve protein synthesis, modifying processes, and several immune responses [30]. Since HSPs can elicit pro-and anti-inflammatory cytokines, these proteins also play pivotal roles in innate immune responses.…”

Section: Discussionmentioning

confidence: 99%

A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis

et al. 2022

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Background and Aim: Feline platynosomiasis, also known as lizard poisoning, is a feline hepatic disease caused by the parasitic trematode Platynosomum fastosum. Since this helminth resides in biliary ducts and gallbladder, the heavy infection can lead to failure of the hepatobiliary system and can be associated with cholangiocarcinoma. The primary diagnostic tool currently used is conventional fecal microscopy. However, low sensitivity of detection could occur in the case of light infection or biliary obstruction. This study aimed to determine the antibody-specific pattern of P. fastosum crude antigen and to identify immunoreactive proteins to develop the immunodiagnostic techniques. Materials and Methods: We investigated potential antigens specific to P. fastosum infection using western blotting. Forty-six samples of cat serum, including 16 P. fastosum-infected sera, eight healthy control sera, and 22 sera infected with other endoparasites were used. The sensitivity, specificity, positive predictive value, and negative predictive value of each band were calculated. Immunoreactive bands with high diagnostic values were further analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the protein components. Results: Using immunoblotting, three proteins of 72 kDa, 53 kDa, and 13 kDa were found to be immunogenic. LC-MS/MS identified these proteins as a 70 kDa heat shock protein, a hypothetical protein (CRM22_002083) (adenosine triphosphate synthase subunit beta), and histone H2B, respectively. Conclusion: This study is the first to reveal three proteins that could be candidates for developing diagnostic tools for feline platynosomiasis.

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“…The remarkable conservation of its primary structure and its expression in a number of organisms make it an interesting target for diagnosis and treatment in various diseases. The therapeutic potential of Hsp70 in schistosomiasis has been explored in the literature [ 35 , 122 , 125 ]. Although Hsp70 is regarded as a potential drug target, its expression is only pronounced in the cercaria-shistosomula stage of the parasite.…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

“…This stage is characterized by a drastic downregulation of the SmCE and upregulation of heat shock proteins (Hsp16, Hsp60 and Hsp70). The upregulation of HSPs in the earliest stages of intra-mammalian schistosomula development is suggested to be in response to the extensive change in niche environments, which include substantial thermal changes between freshwater and the human body [ 35 , 36 ]. A member of the tetraspanins family, S m -TSP-2 is reported to be expressed at this stage and is responsible for tegument formation [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Proteins as Targets in Anti-Schistosomal Drug Discovery and Vaccine Development

2021

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Proteins hardly function in isolation; they form complexes with other proteins or molecules to mediate cell signaling and control cellular processes in various organisms. Protein interactions control mechanisms that lead to normal and/or disease states. The use of competitive small molecule inhibitors to disrupt disease-relevant protein–protein interactions (PPIs) holds great promise for the development of new drugs. Schistosome invasion of the human host involves a variety of cross-species protein interactions. The pathogen expresses specific proteins that not only facilitate the breach of physical and biochemical barriers present in skin, but also evade the immune system and digestion of human hemoglobin, allowing for survival in the host for years. However, only a small number of specific protein interactions between the host and parasite have been functionally characterized; thus, in-depth understanding of the molecular mechanisms of these interactions is a key component in the development of new treatment methods. Efforts are now focused on developing a schistosomiasis vaccine, as a proposed better strategy used either alone or in combination with Praziquantel to control and eliminate this disease. This review will highlight protein interactions in schistosomes that can be targeted by specific PPI inhibitors for the design of an alternative treatment to Praziquantel.

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Heat shock proteins and parasitic diseases: Part 1: Helminths

Cited by 9 publications

References 8 publications

Effects of Multispecies Probiotic on Intestinal Microbiota and Mucosal Barrier Function of Neonatal Calves Infected With E. coli K99

Effects of Multispecies Probiotic on Intestinal Microbiota and Mucosal Barrier Function of Neonatal Calves Infected With E. coli K99

A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis

Proteins as Targets in Anti-Schistosomal Drug Discovery and Vaccine Development

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