Heat Shock Proteins 90 kDa: Immunomodulators and Adjuvants in Vaccine Design Against Infectious Diseases

Corigliano, Mariana G.; Sander, Valeria Analía; López, Edwin F. Sánchez; Morales, Luisa Fernanda Mendoza; Angel, Sérgio O.; Clemente, Marina

doi:10.3389/fbioe.2020.622186

Cited by 13 publications

(17 citation statements)

References 119 publications

(279 reference statements)

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“…HSP 90 kDa (Hsp90) is one of the typical proteins which protect against thermal damage, and it can bind to surface receptors and activate cellular functions related to immune response upon thermal injuries. 52 The Hsp90 expression is significantly upregulated in both the mPDA and CO@mPDA groups when compared with that by the PBS group (Fig. 7A–C), which suggests it has a spontaneously protective effect against thermal injuries in tissues.…”

Section: Resultsmentioning

confidence: 86%

A CO-mediated photothermal therapy to kill drug-resistant bacteria and minimize thermal injury for infected diabetic wound healing

Jin,

Ou,

Zhang

et al. 2023

Biomater. Sci.

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Section: Resultsmentioning

confidence: 86%

A CO-mediated photothermal therapy to kill drug-resistant bacteria and minimize thermal injury for infected diabetic wound healing

Jin,

Ou,

Zhang

et al. 2023

Biomater. Sci.

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“…In this study, we focused on a commercial lipid reagent (lipofectamine) along with a physical method (heat shock under various conditions) for gene transfer in four mammalian cell lines with the same tissue origin (epithelial tissue). Several studies indicated the importance of heat shock proteins such as Hsp90, Hsp70 and Gp96 as an adjuvant for enhancement of humoral and cellular immune responses against infections (Corigliano et al 2021 ; Dalimi and Nasiri 2020 ; Gupta et al 2020 ). Our previous study showed that Hsp27 could be used as a vaccine adjuvant to enhance HIV-1 Nef antigen-specific immunity in DNA-based immunization (Milani et al 2017a ).…”

Section: Discussionmentioning

confidence: 99%

Which one of the thermal approaches (heating DNA or cells) enhances the gene expression in mammalian cells?

et al. 2021

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Objectives Heat treatment as a physical method could increase the cellular uptake of nucleic acids. In this study, the effects of heat shock were evaluated to enhance the transfection efficiency of three plasmid DNAs into HeLa and TC-1 cancerous, and HEK-293 T and Vero non-cancerous cell lines using lipofectamine 2000 reagent. Methods Two methods of cell- and DNA-based heat treatment were used. Heating DNA solution was performed at 94 °C for 5, 10 and 15 min, and also 72 °C for 30, 60 and 120 min, individually. Moreover, heating the cells was done by incubation at 42 °C for 2 h in different times such as before, during and after DNA transfection. Results Our data showed that the conformation of plasmid DNAs was changed at different temperatures with increasing time. The heat-treated plasmid DNAs (94 °C for 10 min or 72 °C for 30 min) indicated higher transfection efficiency than untreated plasmid DNAs ( p < 0.05). Furthermore, heat treatment of cells before and during the transfection was higher than untreated cells ( p < 0.01). Our results demonstrated that DNA transfection efficiency in cancerous cells was less than non-cancerous cells ( p < 0.01). Conclusion Generally, these findings showed that transfection mediated by thermal stimulation could enhance gene transfection in mammalian cell lines.

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“…As a result, efforts have been made to increase vaccine immunogenicity through the integration of appropriate adjuvants, specific antigen targeting and effective carriers. Innovative approaches are imperative in the quest to create non-toxic adjuvants and less immunogenic delivery carriers for treatment of various infectious diseases [1,2]. In recent years, there has been a significant focus on continuous pre-clinical and clinical trials aimed at creating an efficacious HIV vaccine.…”

Section: Introductionmentioning

confidence: 99%

Immunostimulatory effects of Hsp70 fragments and Hsp27 in design of novel HIV‐1 vaccine formulations

Milani,

Akbari,

Pordanjani

et al. 2023

HIV Medicine

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BackgroundHeat shock proteins (HSPs) as an adjuvant induce antigen‐specific immunity through facilitating antigen presentation and stimulating T cells. In this study, the immunostimulatory properties of two major fragments of Hsp70 (N‐Hsp70(aa 1–387) with ATPase property and C‐Hsp70 (aa 508–641) with peptide‐binding capacity) and the full length of Hsp27 as vaccine adjuvants were evaluated to boost HIV‐1 Nef antigen‐specific immunity in both in vitro and in vivo experiments.MethodsAt first, the nanoparticles harbouring DNA fusion constructs (i.e. N‐Hsp70‐Nef, C‐Hsp70‐Nef and Hsp27‐Nef) complexed with HIV Rev (34–50) cell‐penetrating peptide were generated to deliver DNA into the cells. Then, the recombinant Nef, Hsp27‐Nef, N‐Hsp70‐Nef and C‐Hsp70‐Nef proteins were generated in E.coli expression system. Next, the immunostimulatory properties of these fusion constructs were evaluated in both in vitro and in vivo studies. Finally, the secretion of main cytokines from single‐cycle replicable (SCR) HIV‐1 virion‐exposed splenocytes was investigated.ResultsOur data showed that the stable and non‐toxic DNA/Rev nanoparticles could successfully deliver the genes of interest into the cells. Moreover, higher secretion of antibodies and cytokines was detected in mice receiving the Hsp‐Nef constructs than in mice receiving Nef antigen. The C‐Hsp70 was also superior for inducing Nef‐specific Th1 and CTL immunity compared with N‐Hsp70 and Hsp27. The T‐cell activity was maintained in the SCR‐exposed splenocytes, especially the splenocytes of mice receiving the C‐Hsp70‐Nef regimen.ConclusionAltogether, these findings demonstrate the significance of Hsps as enhancers of antigen‐specific immunity. Notably, the C‐Hsp70 region showed better adjuvant properties for inducing cellular immunity in the improvement of HIV‐1 therapeutic vaccines.

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Heat Shock Proteins 90 kDa: Immunomodulators and Adjuvants in Vaccine Design Against Infectious Diseases

Cited by 13 publications

References 119 publications

A CO-mediated photothermal therapy to kill drug-resistant bacteria and minimize thermal injury for infected diabetic wound healing

A CO-mediated photothermal therapy to kill drug-resistant bacteria and minimize thermal injury for infected diabetic wound healing

Which one of the thermal approaches (heating DNA or cells) enhances the gene expression in mammalian cells?

Immunostimulatory effects of Hsp70 fragments and Hsp27 in design of novel HIV‐1 vaccine formulations

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