Heat shock proteins 27, 60 and 70 as prognostic markers of prostate cancer

Abstract

Heat shock proteins (HSPs) protect cells against stress-associated injury and are overexpressed in several malignant tumors. We aimed to investigate their value as prognostic markers in prostate cancer. A tissue microarray (TMA) was constructed of 289 prostate cancers from radical prostatectomy (RP) specimens with median follow-up of 48.9 months. Slides were immunostained for HSP27, HSP60 and HSP70. Intensity and extent of immunoreactivity (IR) and their product (IRp) was evaluated by two observers. The IRp of HSP27 and HSP60, but not of HSP70, significantly predicted biochemical recurrence (p=0.014, 0.034 and 0.160, respectively). Recurrence-free survival in patients with strong HSP27 and HSP60 staining was shorter than in those with weak expression (p=0.019 and 0.001, respectively). IRp of HSP27 and HSP60 correlated with Gleason score (p<0.01). HSP60 was an independent predictor of biochemical recurrence in multivariate analysis, including extraprostatic extension, margin status, seminal vesicle invasion and Gleason score. Weighted kappa for interobserver agreement of HSP27, HSP60 and HSP70 IR was 0.613-0.823 for intensity and 0.584-0.719 for IRp, but only 0.036-0.244 for extent, raising the question whether staining extent should be estimated on TMA. We conclude that HSP27 and HSP60 are predictors of biochemical recurrence after RP.

Quantitative proteomic determination of diethylstilbestrol action on prostate cancer

“…32 The HSPB1 level increases after androgen suppression and is cytoprotective in hormone-refractory PCa. 33,34 In our study, the HSPB1 increase was shown by iTRAQ analysis and qRT-PCR, suggesting its implication in the natural progression of PCa and the tumour response to DES.…”

The dissociation of the Hsp60/pro-Caspase-3 complex by bis(pyridyl)oxadiazole copper complex ( CubipyOXA ) leads to cell death in NCI-H292 cancer cells

“…Frequently, the immunopositivity for Hsp60, in cancerous tissue or circulating cancer cells, correlates with a poor prognosis [49,[51][52][53]. In contrast to these findings, we previously found that the loss of Hsp60 immunopositivity can be related to development and progression of bronchial cancer [54], which reflects the variety of roles that Hsp60 can play.…”

Hsp60 chaperonopathies and chaperonotherapy: targets and agents

“…As such, the chaperonin has become the subject of studies aiming at determining its precise role as etiologic-pathogenic factor and also aiming at inventing new diagnostic and therapeutic ways centered on it. Adrenal gland Adrenal Cushing's tumor [168] Bladder Transitional cell carcinoma; carcinosarcoma [169][170][171] Bone marrow, blood, lymph nodes Acute myeloid leukemia; Hodgkin's lymphoma [155,[172][173][174] Brain Astroglyoma; glioblastoma; meningioma [175][176][177][178][179] Breast Breast ductal carcinoma [180,181] Esophagus Oesophageal carcinoma [182,183] Large bowel Adenocarcinoma [23,169,[184][185][186][187] Liver Hepatocellular carcinoma [188] Lung Bronchial carcinoma [25,26,189,190] Ovary Serous ovarian cancer [191] Prostate Prostate carcinoma [24,[192][193][194] Stomach Gastric maltoma [195] Uterus Exocervical carcinoma [22,196] Adapted from [2]. ATP synthase [197] Dihydrofolatereductase [37,46] Hsp10 [37,41] Human Carbonicanhydrase II [37,198,…”