Heat Shock Protein 90 of <i><scp>B</scp>onamia ostreae</i>: Characterization and Possible Correlation with Infection of the Flat <scp>O</scp>yster, <i><scp>O</scp>strea edulis</i>

Prado-Álvarez, María; Chollet, Bruno; Couraleau, Yann; Morga, Benjamín; Arzul, Isabelle

doi:10.1111/jeu.12031

Cited by 14 publications

(18 citation statements)

References 62 publications

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“…Hemocytes internalyze parasites by endocytosis but B.ostreae seem to actively contribute to its own internalization [3]. It has recently been shown that a heat shock protein 90 (HSP90) is partly involved in parasite internalization [5]. Once internalized, B.ostreae induces some modifications of hemocyte activities including decrease of reactive oxygen species (ROS) production and esterase activities [4,6].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae

Gervais

Chollet

Renault

et al. 2016

Fish & Shellfish Immunology

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The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses. Whatever the host species was, the parasite was engulfed in hemocytes and induced an increase of apoptotic parameters including intracytoplasmic calcium concentration, mitochondrial membrane potential or phosphatidyl-serine externalization as well as ultrastructural modifications. However, the parasite appears more able to infect flat oyster than cupped oyster hemocytes and the apoptotic response was more important against live than dead parasites in the natural host than in C. gigas. Our results suggest that O. edulis specifically responds to B. ostreae by inducing apoptosis of hemocytes.

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Section: Accepted Manuscriptmentioning

confidence: 99%

“…ostreae was involved in its own internalization. Indeed, B. ostreae treatment consisting in blocking actin and HSP90 drastically reduced the percentage of "infected" cells [3,5,6].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae

Gervais

Chollet

Renault

et al. 2016

Fish & Shellfish Immunology

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“…A gene encoding B. ostreae heat shock protein 90 (HSP90) has recently been characterized, and the involvement of this protein in haemocyte invasion was investigated by pre-exposing parasites to radicicol, an HSP90-inhibitor. Haemocytes in contact with radicicol-exposed parasites appeared significantly less infected than haemocytes in contact with nontreated parasites (Prado-Alvarez et al 2013). Flow cytometry was used to demonstrate that live parasites are phagocytosed at higher rates than fluorescent beads .…”

Section: Host−parasite Interactionsmentioning

confidence: 99%

Bonamia parasites: a rapidly changing perspective on a genus of important mollusc pathogens

Engelsma¹,

Culloty²,

Lynch³

et al. 2014

Dis. Aquat. Org.

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Organisms of the genus Bonamia are intracellular protistan parasites of oysters. To date, 4 species have been described (B. ostreae, B. exitiosa, B. perspora and B. roughleyi), al though the status of B. roughleyi is controversial. Introduction especially of B. ostreae and B. exitiosa to naïve host populations has been shown to cause mass mortalities in the past and has had a dramatic impact on oyster production. Both B. ostreae and B. exitiosa are pathogens notifiable to the World Organisation for Animal Health (OIE) and the European Union. Effective management of the disease caused by these pathogens is complicated by the extensive nature of the oyster production process and limited options for disease control of the cultured stocks in open water. This review focuses on the recent advances in research on genetic relationships between Bonamia isolates, geographical distribution, susceptible host species, diagnostics, epizootiology, host− parasite interactions, and disease resistance and control of this globally important genus of oyster pathogens.KEY WORDS: Bonamia ostreae · Bonamia exitiosa · Oyster disease · Phylogenetics · Geographical spread · Diagnostics · Epizootiology · Host−parasite interaction · Bonamiosis Resale or republication not permitted without written consent of the publisher Contribution to DAO Special 7 'Microcell parasites of molluscs'

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“…The inclusion of these functional genes in phylogenomics analyses clarified the phylogenetic position of this organism among rhizarian. Regarding B. ostreae, two actin genes and the HSP90 gene are the unique functional genes described to date and also placed Bonamia representatives among haplosporidians (López-Flores et al 2007, Prado-Alvarez et al 2013. Actin genes are highly conserved, broadly distributed and are widely used in phylogenetic studies of protozoans (Burki et al 2010;Leander and Keeling, 2004;López-Flores et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such asBonamia exitiosa

et al. 2015

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Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where the herein described B. exitiosa actin resolved, offering novel information about the classification of the genus. Our results showed that WGA methodology is a promising and valuable technique to be applied to unculturable protozoans whose genomic material is limited.

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Heat Shock Protein 90 of Bonamia ostreae: Characterization and Possible Correlation with Infection of the Flat Oyster, Ostrea edulis

Cited by 14 publications

References 62 publications

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae

Bonamia parasites: a rapidly changing perspective on a genus of important mollusc pathogens

Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such asBonamia exitiosa

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