Heat shock protein 70 expression induces antitumor immunity during intracellular hyperthermia using magnetite nanoparticles

Ito, Akira; Shinkai, Masashige; Honda, Hiroyuki; Yoshikawa, Kazuhiro; Saga, Shinsuke; Wakabayashi, Toshihiko; Yoshida, Jun; Kobayashi, Takeshi

doi:10.1007/s00262-002-0335-x

Cited by 144 publications

(94 citation statements)

References 34 publications

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“…The heat shock protein 70 (HSP70) gene is upregulated by a wide range of cytotoxic stimulations [12–14]. Indeed, magnetic nanoparticles induce necrotic cell death, which correlates with increased HSP70 expression [15,16]. The IL6 gene is also upregulated by inflammation [17].…”

Section: Resultsmentioning

confidence: 99%

Effects of Titanium Dioxide Nanoparticle Aggregate Size on Gene Expression

Okuda-Shimazaki

Takaku

Kanehira

et al. 2010

IJMS

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Titanium dioxide (titania) nanoparticle aggregation is an important factor in understanding cytotoxicity. However, the effect of the aggregate size of nanoparticles on cells is unclear. We prepared two sizes of titania aggregate particles and investigated their biological activity by analyzing biomarker expression based on mRNA expression analysis. The aggregate particle sizes of small and large aggregated titania were 166 nm (PDI = 0.291) and 596 nm (PDI = 0.417), respectively. These two size groups were separated by centrifugation from the same initial nanoparticle sample. We analyzed the gene expression of biomarkers focused on stress, inflammation, and cytotoxicity. Large titania aggregates show a larger effect on cell viability and gene expression when compared with the small aggregates. This suggests that particle aggregate size is related to cellular effects.

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Section: Resultsmentioning

confidence: 99%

Effects of Titanium Dioxide Nanoparticle Aggregate Size on Gene Expression

Okuda-Shimazaki

Takaku

Kanehira

et al. 2010

IJMS

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“…Complete tumour cell death was observed after 40-min of MHT application at 43 °C. In their second in vitro study, they found that MHT induced HSP-70 expression in the cancer cells [97]. After 30-min of MHT at 42 °C, the T-9 glioma cells displayed cell apoptosis and necrosis.…”

Section: Magnetic Hyperthermia Therapymentioning

confidence: 99%

“…These mice demonstrated reduced tumour growth compared to the control group. In their fourth study, this group demonstrated that T-9 rat glioma cells subcutaneously implanted into the femoral region released tumour-specific HSP-70 following MHT [97]. After 3 weeks of weekly 30-min MHT sessions at 42 °C, the rats that were immunised with T-9-derived HSP-70 had significantly reduced tumour growth compared to the rats that received no immunisation.…”

Section: Magnetic Hyperthermia Therapymentioning

confidence: 99%

Magnetic hyperthermia therapy for the treatment of glioblastoma: a review of the therapy’s history, efficacy and application in humans

Mahmoudi

Bouras

Bozec

et al. 2018

International Journal of Hyperthermia

280

195

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Hyperthermia therapy (HT) is the exposure of a region of the body to elevated temperatures to achieve a therapeutic effect. HT anticancer properties and its potential as a cancer treatment have been studied for decades. Techniques used to achieve a localised hyperthermic effect include radiofrequency, ultrasound, microwave, laser and magnetic nanoparticles (MNPs). The use of MNPs for therapeutic hyperthermia generation is known as magnetic hyperthermia therapy (MHT) and was first attempted as a cancer therapy in 1957. However, despite more recent advancements, MHT has still not become part of the standard of care for cancer treatment. Certain challenges, such as accurate thermometry within the tumour mass and precise tumour heating, preclude its widespread application as a treatment modality for cancer. MHT is especially attractive for the treatment of glioblastoma (GBM), the most common and aggressive primary brain cancer in adults, which has no cure. In this review, the application of MHT as a therapeutic modality for GBM will be discussed. Its therapeutic efficacy, technical details, and major experimental and clinical findings will be reviewed and analysed. Finally, current limitations, areas of improvement, and future directions will be discussed in depth.

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“…But, several clinical trials have indicated that magnetic nanoparticle-mediated hyperthermia (MNHT) is a promising and novel approach to antitumor therapy55. Nanoparticle-mediated hyperthermia differs from RF heating alone given the introduction of magnetic nanoparticles (MNPs) that generate heat when exposed to an alternating magnetic field to cause tumor necrosis tumor and also induce HSP-peptide complex release to elicit systemic antitumor immune responses as reported56. Yanase et al reported that hyperthermic system using magnetic nanoparticles produced HSP70-peptide via necrotic tumor cell death resulting in antitumor immunity, 50% of rats were protected from challenge with T-9 rat glioma cells57.…”

Section: Discussionmentioning

confidence: 99%

Cryo-thermal therapy elicits potent anti-tumor immunity by inducing extracellular Hsp70-dependent MDSC differentiation

Zhu

Zhang

et al. 2016

Sci Rep

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Achieving control of metastatic disease is a long-sought goal in cancer therapy. Treatments that encourage a patient’s own immune system are bringing new hopes in reaching such a goal. In clinic, local hyperthermia and cryoablation have been explored to induce anti-tumor immune responses against tumors. We have also developed a novel therapeutic modality of cryo-thermal treatment by alternating liquid nitrogen (LN2) cooling and radio frequency (RF) heating, and better therapeutic effect was achieved in treating metastatic cancer in animal model. In this study, we investigated the mechanism of systemic immune response elicited by cryo-thermal therapy. In the 4T1 murine mammary carcinoma model, we found that local cryo-thermal therapy resulted in a considerable reduction of distant lung metastases, and improved long-term survival. Moreover, results of tumor re-challenge experiments indicated generation of a strong tumor-specific immune memory after the local treatment of primary tumors. Our further study indicated that cryo-thermal therapy caused an elevated extracellular release of Hsp70. Subsequently, Hsp70 induced differentiation of MDSCs into mature DCs, contributing to the relief of MDSCs-mediated immunosuppression and ultimately the activation of strong anti-tumor immune response. Our findings reveal new insight into the mechanism of robust therapeutic effects of cryo-thermal therapy against metastatic cancers.

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Heat shock protein 70 expression induces antitumor immunity during intracellular hyperthermia using magnetite nanoparticles

Cited by 144 publications

References 34 publications

Effects of Titanium Dioxide Nanoparticle Aggregate Size on Gene Expression

Effects of Titanium Dioxide Nanoparticle Aggregate Size on Gene Expression

Magnetic hyperthermia therapy for the treatment of glioblastoma: a review of the therapy’s history, efficacy and application in humans

Cryo-thermal therapy elicits potent anti-tumor immunity by inducing extracellular Hsp70-dependent MDSC differentiation

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