Effects of Titanium Dioxide Nanoparticle Aggregate Size on Gene Expression

Okuda-Shimazaki, Junko; Takaku, Saiko; Kanehira, Koki; Sonezaki, Shunji; Taniguchi, Akiyohshi

doi:10.3390/ijms11062383

Cited by 90 publications

(106 citation statements)

References 25 publications

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“…In a previous study Park et al (2008) reported that exposure of BEAS-2B cells to titanium dioxide NPs (5, 10, 20 and 40 μg/ml) induced and increased the expression of the oxidative stress-related genes (including heme oxygenase-1, thioredoxin reductase, glutathione-S-transferase, catalase and hypoxia inducible gene). Okuda-Shimazaki et al (2010) found, using quantitative RT-PCR, that titanium dioxide nanoparticle induce inflammation-related genes, including heat shock protein (HSP) and IL-6 in human acute monocytic leukemia cells (THP-1) and human bronchial epithelial cells (NCI-H292). In the present study, the cytotoxicity and genotoxicity potentials of 3 diverse silicon nanoparticles in HPAEpiC and HPPC cells was evaluated by using MTT, LDH and RT-PCR methods, respectively.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Aydın¹,

Türkez²,

Hacımüftüoğlu³

2017

AKU-J. Sci. Eng.

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Silicon nanoparticles are widely used for various applications including environmental, biological, chemical and physical. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly silicon (as silicon carbide, silicon dioxide, silicon nitride) nanoparticles in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these nanoparticles. HPAEpiC and HPPC cells were treated with silicon (0-100 µg/mL), nanoparticles for 72 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array -RT-PCR assay. According to the results of MTT and LDH assays, all tested nanoparticles induced cytotoxicity on both HPAEpiC and HPPC cells in dose-dependent manner. Determining and analyzing the gene expression profiles of HPAEpiC and HPPC cells, silicon nanoparticles showed changes in genes related to apoptosis, DNA damage or repair and oxidative stress. This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles. Silikon Nanopartikül Maruziyetine Bağlı Olarak Oluşan İn Vitro Sitotoksik ve Moleküler Etkiler Anahtar kelimelerAlveolar epitel hücreleri; Genotoksisite; In vitro gen ekspresyonu; Nanotoksisite; Silikon nanopartikülleri ÖzetSilikon nanopartikülleri çevresel, biyolojik, kimyasal ve fiziksel amaçlarla çeşitli alanlarda yaygın olarak kullanılmaktadır. Bu nanopartiküllerin klinik ve endüstriyel alanlarda kullanılabilmesi için güvenilirlikleri özellikle genotoksisite ve sitotoksisite açısından doğrulanmalıdır. Bu yüzden mevcut çalışmada, yaygın olarak kullanılan silikon nanopartiküllerinin ( silikon karbid, silikon dioksit, silikon nitrit) insan alveolar epitel (HPAEpiC) ve farinks (HPPC) hücrelerindeki sitotoksisitesi ve gen ekspresyon profillerindeki değişimlerin araştırılması amaçlanmıştır. HPAEpiC ve HPPC hücreleri 72 saat boyunca silikon nanopartikülleriyle (0-100 µg/mL) muamele edildi. Nanopartiküllerin sitotoksisite değerlendirmeleri için 3-(4,5 dimetylthiazol -2-yl) -2,5 diphenltetrazolium bromide (MTT) ve laktat dehidrogenaz salınım (LDH) yöntemleri kullanılırken; genotoksisite analizi için cDNA array -RT-PCR yöntemi kullanıldı. MTT ve LDH yöntemi sonuçlarına göre, uygulanan bütün test nanopartikülleri hem HPAEpiC hem de HPPC hücre hatlarında doza bağlı olarak sitotoksisiteyi indüklemiştir. HPAEpiC ve HPPC hücrelerinin ilgili genler açısından (apoptozis, DNA fasarı ve tamiri, oksidatif stres) gen ekspresyon profilleri incelendiğinde silikon naopartiküllerinin ekspresyonu değiştirdiği gözlenmiştir. Bu çalışmadan elde edilen nanotoksisiteye bağlı olarak ol...

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Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Aydın¹,

Türkez²,

Hacımüftüoğlu³

2017

AKU-J. Sci. Eng.

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“…While sonication may provide a short-term dispersion in solution, aggregates are still present. It is possible that such aggregates may exert a local acute cytotoxicity (Okuda-Shimazaki et al 2010;Gao et al 2010), but…”

Section: Discussionmentioning

confidence: 99%

“…One study has reported specific uptake patterns J U S T A C C E P T E D depending on the cell line between single and aggregated nanoparticles (Albanese et al 2011), whereas another one showed that aggregated nanoparticles lead to a higher cytotoxicity (Okuda-Shimazaki et al 2010). Magnetic nanoparticles such as NWs possess a permanent magnetic moment that makes them more prone to aggregation in solution (Safi et al 2011), with ultrasonic agitation minimizing this effect temporarily.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and intracellular dissolution of nickel nanowires

Perez

Contreras²,

Vilanova

et al. 2016

Nanotoxicology

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The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 µm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.Just Accepted by Nanotoxicology © 2015 Taylor & Francis. This provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon.DISCLAIMER: The ideas and opinions expressed in the journal's Just Accepted articles do not necessarily reflect those of Taylor & Francis (the Publisher), the Editors or the journal. The Publisher does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of the material contained in these articles. The reader is advised to check the appropriate medical literature and the product information currently provided by the manufacturer of each drug to be administered to verify the dosages, the method and duration of administration, and contraindications. It is the responsibility of the treating physician or other health care professional, relying on his or her independent experience and knowledge of the patient, to determine drug dosages and the best treatment for the patient. Just Accepted articles have undergone full scientific review but none of the additional editorial preparation, such as copyediting, typesetting, and proofreading, as have articles published in the traditional manner. There may, therefore, be errors in Just Accepted articles that will be corrected in the final print and final online version of the article. Any use of the Just Accepted articles is subject to the express understanding that the papers have not yet gone through the full quality control process prior to publication. Cytotoxicity and intracellular dissolution of nickel nanowiresJose E. Perez 1, 2 , Maria F. Contreras 1 , Enrique Vilanova 2 , Laura P. Felix 1, 2 , Michael B.

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“…19 Okuda-Shimazaki et al demonstrated the importance of the aggregation state and showed that larger aggregates of TiO 2 NPs acted more cytotoxically than smaller ones. 20 Phagocytes such as macrophages and monocytes react more strongly to microparticles than to NPs. One study reported higher cell damage for silica microparticles than for NPs, 21 and another study noticed absence of cell damage in THP-1 cells for 30-70 nm silica NPs, while 1000 nm particles acted cytotoxically.…”

Section: Cytotoxicitymentioning

confidence: 99%

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

Frőhlich¹

2012

IJN

1,981

1,416

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Many types of nanoparticles (NPs) are tested for use in medical products, particularly in imaging and gene and drug delivery. For these applications, cellular uptake is usually a prerequisite and is governed in addition to size by surface characteristics such as hydrophobicity and charge. Although positive charge appears to improve the efficacy of imaging, gene transfer, and drug delivery, a higher cytotoxicity of such constructs has been reported. This review summarizes findings on the role of surface charge on cytotoxicity in general, action on specific cellular targets, modes of toxic action, cellular uptake, and intracellular localization of NPs. Effects of serum and intercell type differences are addressed. Cationic NPs cause more pronounced disruption of plasma-membrane integrity, stronger mitochondrial and lysosomal damage, and a higher number of autophagosomes than anionic NPs. In general, nonphagocytic cells ingest cationic NPs to a higher extent, but charge density and hydrophobicity are equally important; phagocytic cells preferentially take up anionic NPs. Cells do not use different uptake routes for cationic and anionic NPs, but high uptake rates are usually linked to greater biological effects. The different uptake preferences of phagocytic and nonphagocytic cells for cationic and anionic NPs may influence the efficacy and selectivity of NPs for drug delivery and imaging.

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Effects of Titanium Dioxide Nanoparticle Aggregate Size on Gene Expression

Cited by 90 publications

References 25 publications

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Cytotoxicity and intracellular dissolution of nickel nanowires

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

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