Heat Shock Protein 70 and Cancer

Liu, Tuoen; Cao, Shousong

doi:10.1007/978-3-319-89551-2_5

Cited by 2 publications

(1 citation statement)

References 94 publications

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“…The first group is HSP70, which is usually not or mildly expressed in normal conditions. However, the expression can be rapidly induced when cells are exposed to heat, cold, or other stressors (Liu and Cao, 2018;Ren et al, 2018). The second group is HSC70, which is expressed constitutively in most human tissues.…”

Section: Heat Shock Protein 70 Familymentioning

confidence: 99%

Functional characterization of heat shock proteins during stress response in pacific white shrimp Penaeus vannamei and brine shrimp Artemia franciscana

Junprung

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Acute hepatopancreatic necrosis disease (AHPND) caused by the bacterium Vibrio parahaemolyticus carrying toxin produced plasmid (VPAHPND), has led to severe mortalities in farmed penaeid shrimp throughout Asia. Our studies demonstrated that a chronic non-lethal heat shock (chronic-NLHS) could enhance resistance of the Pacific white shrimp Penaeus vannamei to VPAHPND infection. Shrimp exposed to chronic-NLHS (28 ?C to 38 ?C, 5 min for 7 days) had higher survival rate (>50%) than that of the control non-heated shrimp (20%) when they were challenged with VPAHPND. The qRT-PCR analysis revealed that the expression of heat shock proteins, LvHSP70 and LvHSP90, as well as other immune-related genes, LvproPO1 and LvCrustin1, were induced upon exposure of shrimp to chronic NLHS. Moreover, LvHSP70 and LvHSP90 gene silencing eradicated the VPAHPND resistance in the chronic-NLHS shrimp and decreased PO activity. Further investigation showed that chronic-NLHS immediately activated the production of LvHSP70 in shrimp hemocytes. Injection of 1.0 nmol recombinant LvHSP70 (rLvHSP70) of injection induced the expression of several immune-related genes (LvMyD88, LvIKK?, LvIKK?, LvCrustin1, LvPEN2, LvPEN3, LvproPO1, LvproPO2, and LvTG1) in the shrimp immune system. Interestingly, rLvHSP70 enhanced P. vannamei resistance to VPAHPND by increasing survival rate from 20% (control group) to >75%. These results suggested that LvHSP70 plays crucial roles in bacterial defense by activating shrimp immune system. In addition, four novel HSP70 family genes designated as HSC70, HSC70-5, BIP, and HYOU1 were identified from the draft Artemia transcriptome database by sequence analysis that contained the specific conserved domain of HSP70 family gene. Expression analysis revealed that in the juvenile state, the transcript level of HSP70 and HSC70 was significantly higher in the selective breeding for induced thermotolerance population (TF12) of Artemia relative to a control population (CF12). Moreover, after a NLHS treatment of TF12 at the nauplii state, HSP70, HSC70, and HSC70-5 transcrips were significantly (P<0.05) up-regulated. In contrast, the expression of the other members of Artemia franciscan HSP70 family showed no significant (P>0.05) induction. Furthermore, SNP polymorphisms of HSP70 and HSC70 genes were investigated and the result identified a SNP at position 171 in HSC70 (C171A; N57K) located at ATP-binding domain which might be potentially associated with the increased thermotolerance. The genotype analysis result showed significant (P<0.01) difference of this SNP between CF12 and TF12. Moreover, the phenotypic analysis by yeast (Saccharomyces cerevisiae) system confirmed that yeast contained the HSC70-N57K plasmid could tolerate high temperature than yeast containing the WT-HSC70 plasmid. As the N57K genotype was more prevalent in TF12 population than that in CF12 suggesting that it can be used in selective breeding for induced thermotolerance in A. franciscana.

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Section: Heat Shock Protein 70 Familymentioning

confidence: 99%

Functional characterization of heat shock proteins during stress response in pacific white shrimp Penaeus vannamei and brine shrimp Artemia franciscana

Junprung

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Sequence and expression analysis of HSP70 family genes in Artemia franciscana

Junprung

Norouzitallab

Vos

et al. 2019

Sci Rep

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Thus far, only one gene from the heat shock protein 70 ( HSP70 ) family has been identified in Artemia franciscana . Here, we used the draft Artemia transcriptome database to search for other genes in the HSP70 family. Four novel HSP70 genes were identified and designated heat shock cognate 70 ( HSC70 ), heat shock 70 kDa cognate 5 ( HSC70-5 ), Immunoglobulin heavy-chain binding protein (BIP) , and hypoxia up-regulated protein 1 ( HYOU1 ). For each of these genes, we obtained nucleotide and deduced amino acid sequences, and reconstructed a phylogenetic tree. Expression analysis revealed that in the juvenile state, the transcription of HSP70 and HSC70 was significantly ( P < 0.05) higher in a population of A . franciscana selectively bred for increased induced thermotolerance (TF12) relative to a control population (CF12). Following non-lethal heat shock treatment at the nauplius stage, transcription of HSP70 , HSC70 , and HSC70-5 were significantly ( P < 0.05) up-regulated in TF12. In contrast, transcription of the other HSP70 family members in A . franciscana ( BIP , HYOU1 , and HSPA4 ) showed no significant ( P > 0.05) induction. Gene expression analysis demonstrated that not all members of the HSP70 family are involved in the response to heat stress and selection and that especially altered expression of HSC70 plays a role in a population selected for increased thermotolerance.

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Heat Shock Protein 70 and Cancer

Cited by 2 publications

References 94 publications

Functional characterization of heat shock proteins during stress response in pacific white shrimp Penaeus vannamei and brine shrimp Artemia franciscana

Functional characterization of heat shock proteins during stress response in pacific white shrimp Penaeus vannamei and brine shrimp Artemia franciscana

Sequence and expression analysis of HSP70 family genes in Artemia franciscana

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