Sequence and expression analysis of HSP70 family genes in Artemia franciscana

Abstract

Thus far, only one gene from the heat shock protein 70 ( HSP70 ) family has been identified in Artemia franciscana . Here, we used the draft Artemia transcriptome database to search for other genes in the HSP70 family. Four novel HSP70 genes were identified and designated heat shock cognate 70 ( HSC70 ), heat shock 70 kDa cognate 5 ( HSC70-5 ), Immunoglobulin heavy-chain binding protein (BIP) , and hypoxia up-regulated protein 1 ( HYOU1 ). For each of these genes, we obtained nucleotide and deduced amino acid sequences, and reconstructed a phylogenetic tree. Expression analysis revealed that in the juvenile state, the transcription of HSP70 and HSC70 was significantly ( P < 0.05) higher in a population of A . franciscana selectively bred for increased induced thermotolerance (TF12) relative to a control population (CF12). Following non-lethal heat shock treatment at the nauplius stage, transcription of HSP70 , HSC70 , and HSC70-5 were significantly ( P < 0.05) up-regulated in TF12. In contrast, transcription of the other HSP70 family members in A . franciscana ( BIP , HYOU1 , and HSPA4 ) showed no significant ( P > 0.05) induction. Gene expression analysis demonstrated that not all members of the HSP70 family are involved in the response to heat stress and selection and that especially altered expression of HSC70 plays a role in a population selected for increased thermotolerance.

Mechanisms of Desiccation Tolerance: Themes and Variations in Brine Shrimp, Roundworms, and Tardigrades

“…The 70 kDa heat shock proteins (Hsp70s) are a family of ATPdependent chaperones essential for protein folding. Knockdown of one family member, Hsp70, in Artemia sensitizes developing nauplii to heat stress and infection with the bacterium Vibrio campbellii (Iryani et al, 2017;Junprung et al, 2019). Knockdown of Hsp70 has a modest effect on survival of desiccated and frozen cysts (Iryani et al, 2020).…”

Transcriptional profiling of MED Bemisia tabaci exposed to thermal stress and verification of HSP70 expression

“…Such as, in Artemia franciscana, Following non-lethal heat shock treatment at the nauplius stage, transcription of HSP70, HSC70, and HSC70-5 were signifcantly upregulated. In contrast, the other HSP70 family members in A. franciscana (BIP, HYOU1, and HSPA4) showed no signifcant induction (Junprung et al 2019). And in Melitaea cinxia, the high expression of McHsp70-1, McHsp70-2 and McHsc70-2 can be induced at 40°C, but , which also belong to the HSP70 family, do not respond to high temperature stress (Luo et al 2015).…”

“…In contrast, the other HSP70 family members in A. franciscana (BIP, HYOU1, and HSPA4) showed no signifcant induction (Junprung et al . 2019). And in Melitaea cinxia , the high expression of McHsp70–1 , McHsp70–2 and McHsc70–2 can be induced at 40°C, but McHsp70–3 , McHsp70–4 and McHsp70–5 , which also belong to the HSP70 family, do not respond to high temperature stress (Luo et al .…”

Betulinic Acid Modulates the Expression of HSPA and Activates Apoptosis in Two Cell Lines of Human Colorectal Cancer

“…We found that the HSPA expression was markedly decreased in both HCT116 (at 5 µM) and SWU480 cells (at 10 µM), although there was a slight increase in HSPA expression at lower concentrations (1.25 and 2.5 µM). According to previous studies, the expression of HSPA can be induced by multiple mechanisms such as heat, glucose-regulated protein 78 (GRP78), a member of the HSPA family, and reactive oxygen species (ROS) [43][44][45]. Interestingly, the BA treatment significantly upregulated GRP78-mediated endoplasmic reticulum (ER) stress in breast cancer cells [46].…”