Heat, Salinity, and Acidity, Commonly Upregulate A1aB1b Proglycinin in Soybean Embryonic Axes

Arce‐Paredes, Patricia; Mora‐Escobedo, Rosalva; Luna-Arias, Juan Pedro; Mendoza‐Hernández, Guillermo; Rojas-Espinosa, O

doi:10.5772/14227

Cited by 7 publications

(8 citation statements)

References 48 publications

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“…Seed germination is a critical phase in the life history of plants; therefore, salt tolerance during the germination phase may be especially critical for successful establishment and productivity of soybean in saline soils . High salinity negatively affect germination, growth, nodulation, seed quality and quantity, thus reducing the yield of soybean production .Therefore, it is of great interest to understand the genetic basis of salinity tolerance traits in soybean, mainly at seed germination stage .…”

Section: Discussionmentioning

confidence: 99%

“…It is evident that the seedling stage of soybean is much more sensitive to salinity than the germination . For instance, soybean seedling growth is suppressed when the concentration of Na + in tissues exceeded 6% of the fresh weight, whereas germination remains possible even when the concentration of Na + exceeded 9% of fresh weight .…”

Section: Discussionmentioning

confidence: 99%

“…Similar results was reported recently , suggesting that storage proteins might not only function as seed storage reserves but also have additional roles in plant defense. Almost 90% of the proteins in soybean are storage proteins, which mostly consist of β‐conglycinin (7S) and glycinin (11S) . Recent proteomics studies have revealed the presence of several isoforms of glycinin and conclycinin in the embryonic axis of soybean .…”

Section: Discussionmentioning

confidence: 99%

“…Recently several SRGs have been investigated in soybean . For instance, analyzing more than 190 accessions of soybean, Kan et al.…”

Section: Introductionmentioning

confidence: 99%

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Shotgun proteomic analysis of soybean embryonic axes during germination under salt stress

et al. 2016

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Seed imbibition and radicle emergence are generally less affected by salinity in soybean than in other crop plants. In order to unveil the mechanisms underlying this remarkable salt tolerance of soybean at seed germination, a comparative label-free shotgun proteomic analysis of embryonic axes exposed to salinity during germination sensu stricto (GSS) was conducted. The results revealed that the application of 100 and 200 mmol/L NaCl stress was accompanied by significant changes (>2-fold, P<0.05) of 97 and 75 proteins, respectively. Most of these salt-responsive proteins (70%) were classified into three major functional categories: disease/defense response, protein destination and storage and primary metabolism. The involvement of these proteins in salt tolerance of soybean was discussed, and some of them were suggested to be potential salt-tolerant proteins. Furthermore, our results suggest that the cross-protection against aldehydes, oxidative as well as osmotic stress, is the major adaptive response to salinity in soybean.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Recently several SRGs have been investigated in soybean . For instance, analyzing more than 190 accessions of soybean, Kan et al.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Shotgun proteomic analysis of soybean embryonic axes during germination under salt stress

et al. 2016

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“…Two-dimensional DIGE was performed at Applied Biomics (Hayward, CA, USA) following typical methods [ 19 , 20 ]. Briefly, cell lysates, were denatured by equal volume addition of lysis buffer containing 7M urea, 2M thio urea, 4% 3-((3-cholamidopropyl)dimethyl ammonio)-1-propanesulfonate(CHAPS) followed by addition of 30 mM Tris-HCl, pH 8.8, at a 5:1 volume ratio lysis buffer: plasma.…”

Section: Methodsmentioning

confidence: 99%

Identification of the Transmembrane Glucose Regulated Protein 78 as a Biomarker for the Brain Cancer Glioblastoma Multiforme by Gene Expression and Proteomic Studies.

Hyman

Evans

et al. 2013

J Membra Sci Technol

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The prognosis of patients with Glioblastoma Multiforme (GBM), the most malignant adult glial brain tumor, remains poor in spite of advances in treatment procedures, including surgical resection, irradiation and chemotherapy. Genetic heterogeneity of GBM warrants extensive studies to gain a thorough understanding of the biology of this tumor. While there have been several studies of global transcript profiling of glioma with the identification of gene signatures for diagnosis and disease management, translation into clinics is yet to happen. In the present study, we report a novel proteomic approach by using two-dimensional difference gel electrophoresis (2D-DIGE) followed by spot picking and analysis of proteins/peptides by Mass Spectrometry. We report Glucose Regulated Protein 78 (GRP78) as a differentially expressed protein in the GBM cell line compared to human normal Astrocyte cells.In addition to proteomic studies, we performed microarray analysis which further confirmed up regulation of GRP78 in GBM cells compared to human normal Astrocyte cells.GRP78 has long been recognized as a molecular chaperone in the endoplasmic reticulum (ER) and can be induced by the ER stress response. Besides its location in the ER, GRP78 has been found in cell plasma membrane, cytoplasm, mitochondria, nucleus and other cellular secretions. GRP78 is implicated in tumor cell proliferation, apoptosis resistance, immune escape, metastasis and angiogenesis, and its elevated expression usually correlates with a variety of tumor micro environmental stresses, including hypoxia, glucose deprivation, lactic acidosis and inflammatory response. GRP78 protein acts as a centrally located sensor of stress, which senses and facilitates the adaptation to the tumor microenvironment.Our findings showed differential expression of this gene in brain cancer GBM and thus confirm similarities in findings in existing transcriptional and translational studies. Thus, these findings could be of further importance for diagnostic, therapeutic and prognostic approaches for dealing with this highly malignant cancer.

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Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

et al. 2015

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Heat shock proteins (HSPs) exist extensively in eukaryotes and are conserved molecular chaperones with important contribution to plant's survival under environmental stresses. Here, the cloning and characterization of one complementary DNA (cDNA) designated as BcHSP70 from young seedlings of Brassica campestris were reported in the present work. Bioinformatic analysis revealed that BcHSP70 belongs to the plant HSP gene family and had the closest relationship with HSP70-4 from Arabidopsis thaliana. Constitutive overexpression of BcHSP70 in tobacco obviously conferred tolerance to heat stress by affecting different plant physiological parameters. In our study, transgenic tobaccos exhibited higher chlorophyll content than wild-type control when exposed to heat stress. Superoxide dismutase (SOD) and peroxidase (POD) activities, which were helpful to decrease the damage to the membrane system, were significantly higher in transformants compared to wild-type lines. Meanwhile, lower comparative electrical conductivity and malondialdehyde (MDA) content and higher proline and soluble sugar accumulation were found in transgenic tobaccos than in wild-type lines. All these above results indicated that this isolated BcHSP70 cDNA owned the ability to improve the tolerance to heat stress in transgenic tobacco, which provides helpful information and good basement to culture new robust B. campestris variety resistant to high-temperature stress by molecular breeding in the future.

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Heat, Salinity, and Acidity, Commonly Upregulate A1aB1b Proglycinin in Soybean Embryonic Axes

Cited by 7 publications

References 48 publications

Shotgun proteomic analysis of soybean embryonic axes during germination under salt stress

Shotgun proteomic analysis of soybean embryonic axes during germination under salt stress

Identification of the Transmembrane Glucose Regulated Protein 78 as a Biomarker for the Brain Cancer Glioblastoma Multiforme by Gene Expression and Proteomic Studies.

Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

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