Heat induced collagen denaturation and its effects on bone mechanical integrity

Wang, X.; Bank, Ruud A.; Shen, Xinmei; Koppele, J.M. te; Athanasiou, K. A.; Agrawal, C. Mauli

doi:10.1109/iembs.1999.802571

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…As reported by Wang et al [1,2], non-calcified collagen molecules in bone degenerate at about 43 0 C and calcified collagen molecules degenerate at about 150 0 C. It is interesting to note that when temperature is at 37 0 C, both E values in osteons and interstitial lamellae have significant decrease of 40% and 57%, respectively. The H values lies with the same pattern as E values that a decrease of 15.6% and 27.4%, respectively.…”

Section: Resultsmentioning

confidence: 53%

Assessing Heat-Treatment Effects on Bovine Cortical Bones by Nanoindentation

Lau

et al. 2011

AMR

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Among different sterilization methods, heat-treatment of bone is recognized as one of the simple and practical methods to lower the human immunodeficiency virus (HIV) infection and overcome the risks of rejection and disease transfer from allograft and xenograft during bone transplantation. In order to best characterize the micro-structural mechanical property of bone after heat treatment, the nanoindentation technique was applied in this study to measure the localized elastic modulus and hardness for interstitial lamellae and osteons lamellae of bovine cortical bones at temperature 23°C (room temperature-pristine specimen), 37°C, 90°C, 120°C and 160°C, respectively. The elastic modulus (E) and hardness (H) of interstitial lamellae obtained higher values as compared with osteons lamellae which show that interstitial lamellae are more stiff and mineralized than osteons. Moreover, as a specimen pre-heat treated at 90°C, the E and H values of interstitial lamellae and osteons were closed to a pristine specimen. For a specimen pre-heat treated at 120°C, both interstitial lamellae and osteons obtained an increase in E and H values. As a specimen pre-heat treated at 160°C, the interstitial lamellae and osteons obtained a slight decrease in E and H values. These findings are correlated to results reported by other researchers [1, 2] that calcified collagen molecules starts to degenerate at about 120°C and complete at 160°C. Interestingly, when a specimen was pre-heat treated at 37°C, both interstitial lamellae and osteons obtained significant decreases in E values of 57% and 40%, respectively as compared to the pristine specimen; while in H values, there was a decrease of 27.4% and 15%, respectively. Thus, this paper will investigate the mechanical properties of bovine cortical bones under various temperature ranges by nanoindentation technique.

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Section: Resultsmentioning

confidence: 53%

Assessing Heat-Treatment Effects on Bovine Cortical Bones by Nanoindentation

Lau

et al. 2011

AMR

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“…Placing a value on the denaturation temperature of collagen is highly dependent on the species. Vertebrates, for example, have at least 27 different types of collagen (Myllyharju and Kivirikko, 2004) with denaturation temperatures ranging from the approximately 30 °C up to 160 °C depending on species and location within the body (Zhang, Liu, and Li, 2009); (Wang et al, 1999). C. reniformis belongs to a far less well studied phylum (Porifera) leading to a dearth of information on its collagen.…”

Section: Introductionmentioning

confidence: 99%