Identification of a suitable sterilisation method for collagen derived from a marine Demosponge

Palmer, Iwan; Clarke, Sonya; Nelson, Jane; Schatton, Wolfgang; Dunne, Nicholas; Buchanan, Fraser

doi:10.1504/ijnbm.2012.050306

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…All samples, barring VP‐PMMA, which was produced under aseptic conditions as per the manufacturer's instructions, were sterilized using γ‐irradiation in accordance with ISO‐11137‐1:2006 (Isotron Applied Sterilisation Technologies, Synergy Health, Swindon, UK). Sterilization using γ‐irradiation has been reported as the most suitable sterilization technique for collagen extracted from C. reniformis …”

Section: Methodsmentioning

confidence: 99%

“…Sterilization using girradiation has been reported as the most suitable sterilization technique for collagen extracted from C. reniformis. 39 In vitro evaluation In vitro evaluation was carried out using human bone marrow stromal cells (hBMSCs), which are primary cells similar to those that would be in contact with the implant in vivo and are capable of differentiating along the osteogenic lineage. 40 Following informed patient consent, approximately 6 mL of bone marrow was collected from the tibial medullary canal of a 19-year-old male undergoing a tibial intramedullary rod fixation procedure at Belfast's Royal Victoria Hospital.…”

Section: Methodsmentioning

confidence: 99%

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Biocompatibility of calcium phosphate bone cement with optimized mechanical properties

Palmer

Nelson

Schatton³

et al. 2015

J Biomed Mater Res

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The broad aim of this work was to investigate and optimize the properties of calcium phosphate bone cements (CPCs) for use in vertebroplasty to achieve effective primary fixation of spinal fractures. The incorporation of collagen, both bovine and from a marine sponge (Chondrosia reniformis), into a CPC was investigated. The biological properties of the CPC and collagen–CPC composites were assessed in vitro through the use of human bone marrow stromal cells. Cytotoxicity, proliferation, and osteoblastic differentiation were evaluated using lactate dehydrogenase, PicoGreen, and alkaline phosphatase activity assays, respectively. The addition of both types of collagen resulted in an increase in cytotoxicity, albeit not to a clinically relevant level. Cellular proliferation after 1, 7, and 14 days was unchanged. The osteogenic potential of the CPC was reduced through the addition of bovine collagen but remained unchanged in the case of the marine collagen. These findings, coupled with previous work showing that incorporation of marine collagen in this way can improve the physical properties of CPCs, suggest that such a composite may offer an alternative to CPCs in applications where low setting times and higher mechanical stability are important. © 2015 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. 104B:308–315, 2015.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Biocompatibility of calcium phosphate bone cement with optimized mechanical properties

Palmer

Nelson

Schatton³

et al. 2015

J Biomed Mater Res

Self Cite

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“…Collagens from the demosponge Chondrosia reniformis (Nardo 1847) have received attention from researchers since 1970 [ 113 , 117 , 118 , 119 ] due to their diversity (type IV collagen, fibrillar and nonfibrillar collagens) [ 120 ] and interesting structural [ 121 ], physicochemical [ 122 ], and ecophysiological properties [ 123 , 124 , 125 , 126 , 127 ]. For example, slices of fibrillar collagen incubated with collagenase are not modified even after 48 h of incubation, and do not show any changes in the aspect, consistency, or fine structure of the fibrils.…”

Section: Chondrosia Collagensmentioning

confidence: 99%

Collagens of Poriferan Origin

Ehrlich

Wysokowski

Żółtowska-Aksamitowska

et al. 2018

Marine Drugs

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The biosynthesis, structural diversity, and functionality of collagens of sponge origin are still paradigms and causes of scientific controversy. This review has the ambitious goal of providing thorough and comprehensive coverage of poriferan collagens as a multifaceted topic with intriguing hypotheses and numerous challenging open questions. The structural diversity, chemistry, and biochemistry of collagens in sponges are analyzed and discussed here. Special attention is paid to spongins, collagen IV-related proteins, fibrillar collagens from demosponges, and collagens from glass sponge skeletal structures. The review also focuses on prospects and trends in applications of sponge collagens for technology, materials science and biomedicine.

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“…Gamma ( ) irradiation is the most common sterilisation technique for orthopaedic implants [28], as well as various other fields including food preservation and pharmaceutical production [29], with between 25 and 40 kGy doses of radiation known to ensure sterility. Energy from photons of radiation is transferred to the electrons in the exposed material [30]. The highly excited electrons then yield high doses of free radicals which disrupt the DNA double helix of any microorganism present.…”

Section: Introductionmentioning

confidence: 99%

Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes

Hodder

Duin

Kilian

et al. 2019

J Mater Sci: Mater Med

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For both the incorporation of cells and future therapeutic applications the sterility of a biomaterial must be ensured. However, common sterilisation techniques are intense and often negatively impact on material physicochemical attributes, which can affect its suitability for tissue engineering and 3D printing. In the present study four sterilisation methods, autoclave, supercritical CO2 (scCO2) treatment, UV-and gamma () irradiation were evaluated regarding their impact on material properties and cellular responses. The investigations were performed on methyl cellulose (MC) as a component of an alginate/methyl cellulose (alg/MC) bioink, used for bioprinting embedded bovine primary chondrocytes (BPCs). In contrast to the autoclave, scCO2 and UV-treatments, the-irradiated MC resulted in a strong reduction in alg/MC viscosity and stability after extrusion which made this method unsuitable for precise bioprinting. Gel permeation chromatography analysis revealed a significant reduction in MC molecular mass only after-irradiation, which influenced MC chain mobility in the Ca 2+crosslinked alginate network as well as gel composition and microstructure. With regard to cell survival and proteoglycan matrix production, the results determined UV-irradiation and autoclaving as the best candidates for sterilisation. The scCO2-treatment of MC resulted in an unfavourable cell response indicating that this method needs careful optimisation prior to application for cell encapsulation. As proven by consistent FT-IR spectra, chemical alterations could be excluded as a cause for the differences seen between MC-treatments on alg/MC behaviour. This investigation provides knowledge for the development of a clinically appropriate 3D-printing-based fabrication process to produce bioengineered tissue for cartilage regeneration.

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Identification of a suitable sterilisation method for collagen derived from a marine Demosponge

Cited by 14 publications

References 42 publications

Biocompatibility of calcium phosphate bone cement with optimized mechanical properties

Biocompatibility of calcium phosphate bone cement with optimized mechanical properties

Collagens of Poriferan Origin

Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes

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