Health Risks of Space Exploration: Targeted and Nontargeted Oxidative Injury by High-Charge and High-Energy Particles

Li, Min; Gonon, Géraldine; Buonanno, Manuela; Autsavapromporn, Narongchai; Toledo, Sonia M. de; Pain, Debkumar; Azzam, Edouard I.

doi:10.1089/ars.2013.5649

Cited by 42 publications

(34 citation statements)

References 279 publications

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“…However, the intensity of this interaction depends on cell type, cellular growth state, type of radiation, and the biological endpoint being measured [1]. Recent reports have demonstrated the role of indirect effects of radiation in neurodegeneration [2].…”

Section: Introductionmentioning

confidence: 99%

Glial U87 cells protect neuronal SH-SY5Y cells from indirect effect of radiation by reducing oxidative stress and apoptosis

Saeed¹,

Xie²,

Xu³

et al. 2015

ABBS

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Recent studies have demonstrated the role of indirect effect of radiation in neurodegeneration. However, the role of glial cells in neuroprotection against indirect effect of radiation is still not clear, although they are known to protect neurons under stress conditions in central nervous system. Our study showed that indirect effect of radiation increased the oxidative stress that further enhances the expression of key apoptotic proteins and leads to neuronal cell death. We also investigated the indirect effect of radiation on neuronal cells in the presence of glial cells in a transwell co-culture system, while our analysis was focused on neuronal cells. Irradiated cell-conditioned medium (ICCM) was used as source of indirect radiation and neuroprotective effect was analyzed by various endpoints. It was observed that ICCM-induced reactive oxidative species level was significantly reduced in SH-SY5Y cells co-cultured with glial U87 cells, which might help to maintain the integrity of mitochondrial membrane potential. Increased levels of antioxidant enzyme superoxide dismutase and antioxidant glutathione were observed in SH-SY5Y cells co-cultured with glial U87 cells. Moreover, it was also observed that co-culture with glial cells inhibits the expression of ICCM-induced apoptotic proteins, i.e. Bax, cytochrome c, and caspase-3 in SH-SY5Y cells. Hence, it can be speculated that in co-culture system glial cells may protect the neuronal SH-SY5Y cells by reducing the ICCM-induced oxidative stress and apoptotic death.

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Section: Introductionmentioning

confidence: 99%

Glial U87 cells protect neuronal SH-SY5Y cells from indirect effect of radiation by reducing oxidative stress and apoptosis

Saeed¹,

Xie²,

Xu³

et al. 2015

ABBS

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“…While X-rays and c rays are sparsely ionizing radiations, therapeutic a-particles or energetic heavy ions are densely ionizing (Hall and Giaccia 2006). Although the focus of this study is on the effects of a-particles on cardiomyocyte differentiation from human pluripotent stem cells, the knowledge gained is relevant to other densely ionizing radiations such as energetic heavy ions used in cancer therapy or to which astronauts may be exposed during prolonged travel in deep space: a-particles and energetic heavy ions share many biophysical characteristics (Li et al 2014). Our study on human cells expands and complements recent studies on cardiac differentiation by therapeutic X-rays and energetic carbon ions in mouse embryonic stem cells, which show a likely impact on murine cardiac differentiation (Helm et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Effect of densely ionizing radiation on cardiomyocyte differentiation from human‐induced pluripotent stem cells

Baljinnyam

Venkatesh

Gordan

et al. 2017

Physiological Reports

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The process of human cardiac development can be faithfully recapitulated in a culture dish with human pluripotent stem cells, where the impact of environmental stressors can be evaluated. The consequences of ionizing radiation exposure on human cardiac differentiation are largely unknown. In this study, human‐induced pluripotent stem cell cultures (hiPSCs) were subjected to an external beam of 3.7 MeV α‐particles at low mean absorbed doses of 0.5, 3, and 10 cGy. Subsequently, the hiPSCs were differentiated into beating cardiac myocytes (hiPSC‐CMs). Pluripotent and cardiac markers and morphology did not reveal differences between the irradiated and nonirradiated groups. While cell number was not affected during CM differentiation, cell number of differentiated CMs was severely reduced by ionizing radiation in a dose‐responsive manner. β‐adrenergic stimulation causes calcium (Ca2+) overload and oxidative stress. Although no significant increase in Ca2+ transient amplitude was observed in any group after treatment with 1 μmol/L isoproterenol, the incidence of spontaneous Ca2+ waves/releases was more frequent in hiPSC‐CMs of the irradiated groups, indicating arrhythmogenic activities at the single cell level. Increased transcript expression of mitochondrial biomarkers (LONP1, TFAM) and mtDNA‐encoded genes (MT‐CYB, MT‐RNR1) was detected upon differentiation of hiPSC‐CMs suggesting increased organelle biogenesis. Exposure of hiPSC‐CM cultures to 10 cGy significantly upregulated MT‐CYB and MT‐RNR1 expression, which may reflect an adaptive response to ionizing radiation. Our results indicate that important aspects of differentiation of hiPSCs into cardiac myocytes may be affected by low fluences of densely ionizing radiations such as α‐particles.

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“…空间辐射的剂量率低, 但是旁效应不容忽视. 0.2 cGy 的铁离子照射, 仅有~1%的细胞被直接辐照, 却可以检测到明显的蛋白羰基化, 1 cGy 的铁离子照射即可诱导明显的脂质过氧化, 提示旁效应的存在 [20] . 旁效应与辐照品质等相关 [21] , 其分子机制已经有诸多阐述 [22] , 近年来研究发现, 非编码 RNA 等也参与旁效应信号的调控 [23] .…”

Section: 空间辐射的来源和特点unclassified

Challenges and opportunities for the space radiobiology research in China

Zhou¹,

Hu²

2019

Chin. Sci. Bull.

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Health Risks of Space Exploration: Targeted and Nontargeted Oxidative Injury by High-Charge and High-Energy Particles

Cited by 42 publications

References 279 publications

Glial U87 cells protect neuronal SH-SY5Y cells from indirect effect of radiation by reducing oxidative stress and apoptosis

Glial U87 cells protect neuronal SH-SY5Y cells from indirect effect of radiation by reducing oxidative stress and apoptosis

Effect of densely ionizing radiation on cardiomyocyte differentiation from human‐induced pluripotent stem cells

Challenges and opportunities for the space radiobiology research in China

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