“…Mainly for its health benefits by lowering cholesterol levels, there is an increasing interest for RYR around the world (Kalaivani, Sabitha, Kalaiselvan, & Rajasekaran, 2010). In RYR, the levels of monacolin K, which is formed during the fermentation process and inhibits HMG-CoA reductase (involved in cholesterol synthesis), are crucial to achieve the desirable functional properties, but since fungal activity is also associated with the production of CIT, there is a demand for the optimization of the process (Kalaivani et al, 2010;Kiebooms, Huybrechts, Thiry, Tangni, & Callebaut, 2016;Liao, Chen, Lin, Chiueh, & Shih, 2014). The selection of Monascus strains and the application of mutagenesis techniques to increase the monacolin K/CIT ratio produced during fermentation are being explored (Kanpiengjai, Mahanwan, Pengnoi, Lumyong, & Khanongnuch, 2018;Tsukahara, Shinzato, Tamaki, Namihira, & Matsui, 2009).…”