Healing capacity of bone marrow mesenchymal stem cells versus platelet-rich fibrin in tibial bone defects of albino rats: an in vivo study

Rady, Dina; Mubarak, Rabab; Moneim, Rehab A. Abdel

doi:10.12688/f1000research.15985.1

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…21 An additional empty tube was implanted as a control in an attempt to control variables, avoid selection bias, and neutralize any confounders that might affect the results. 16 In this study, stromal inflammatory response (mononuclear cells), vascular changes, fibrous tissue formation, and the presence of MNGCs in the subcutaneous tissues of the rats were evaluated. In all subgroups, the results showed a moderate to severe stromal inflammatory response presented as the recruitment of inflammatory cells, numerous blood vessels and irregular collagen fibers with the presence of MNGCs.…”

Section: Discussionmentioning

confidence: 99%

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Biocompatibility assessment of different root-end filling materials implanted subcutaneously in rats: An in vivo study

Rady¹,

Rahman²,

El-Mallah³

et al. 2021

Dent. Med. Probl.

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Background. Root-end filling materials are used in surgical endodontic treatment to seal the teeth periapically. Ideally, these materials should prevent bacterial leakage by tightly sealing the canal, be biocompatible with the periapical tissues, and preferably stimulate the regeneration of dentin, contributing to the success of treatment.Objectives. The purpose of this study was to evaluate and compare the biocompatibility of the Gutta-Flow ® Bioseal cement in relation to MTA Angelus ® and Zical ® after implantation into the subcutaneous tissue of rats. Material and methods.Eighteen male albino rats were used in the study. Four polyethylene tubes were implanted in the backs of the rats (3 tubes containing the test materials and 1 empty tube as a control). Nine animals were sacrificed at each interval of 7 and 30 days, and the implants were removed with the surrounding tissue. The samples were evaluated for stromal inflammatory response, fibrous tissue formation, vascular reactivity, and the presence of multinucleated giant cells (MNGCs).Results. On day 7, the capsules in all subgroups revealed moderate to severe inflammatory reactions with the presence of inflammatory cells, multiple irregular collagen fibers, dilated blood vessels, and MNGCs. However, on day 30, tissue organization was more evident with a reduction in the inflammatory response. In this time interval, the tissue in contact with GuttaFlow Bioseal showed progressive healing with a wellformed fibrous capsule. Conversely, the tissue close to MTA Angelus revealed a fibrous capsule of limited organization with mild pericapsular fibrosis and vascular congestion. Zical showed a mild to moderate persistent inflammatory reaction and vascular reactivity.Conclusions. The 3 cements demonstrated more severe irritation at the beginning that became milder with time. GuttaFlow Bioseal yielded better tissue organization than MTA Angelus and Zical. Thus, these findings strongly suggest that GuttaFlow Bioseal is a promising material for root-end filling.

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Section: Discussionmentioning

confidence: 99%

“…On days 7 and 30 after implantation, the animals were euthanized by intracardiac overdose of sodium thiopental (80 mg/kg b.w.). 16…”

Section: Animal Study Designmentioning

confidence: 99%

Biocompatibility assessment of different root-end filling materials implanted subcutaneously in rats: An in vivo study

Rady¹,

Rahman²,

El-Mallah³

et al. 2021

Dent. Med. Probl.

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“…The sample size was calculated based on the data of reference Rady et al, 22 that the maximum difference between the three groups mean is 1.08 and the estimated pooled standard deviation is 0.96. Assuming the alpha error 0.05 and power 0.8 a number of 17 cases per group is required adding 3 cases for anticipated missing data it results in 20 cases per group a total of 60 cases.…”

Section: Sample Size Calculationmentioning

confidence: 99%

Evaluation of the Regenerative Effect of Rabbit BMMSCs and ADSCs. An In Vivo Study

Shawky

Hassouna

2021

Ain Shams Dental Journal

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Background: The malignant transformation potential of oral lichen planus (OLP) is a subject of great controversy. Aims: The present study aimed to assess immunohistochemically Survivin and CD146 expressions in OLP and different grades of oral squamous cell carcinoma (OSCC). Methods: Fifty specimens of OLP and OSCC, twenty-five each, were included in the present study. Immunohistochemical staining with anti-Survivin and anti-CD146 primary antibodies was performed. Area fraction of immunopositivity was measured by image analysis software followed by statistical analysis using ANOVA and Bonferroniʾs post hoc tests. Results: All cases of OLP were immunopositive for Survivin and CD146 while in OSCC all cases were immunopositive for Survivin and only twenty cases of well and moderately differentiated OSCC were immunopositive for CD146. All cases of poorly differentiated OSCC were immunonegative for CD146. Statistically significant difference in Survivin and CD146 expressions were detected in OLP and all grades of OSCC (P < 0.001). Moreover, statistically significant difference in expression of both markers was detected when compared with different histopathological grades of OSCC. A statistically significant inverse correlation between Survivin and CD146 immunoexpression in both lesions was found. Conclusion: Survivin could be an early diagnostic marker that predicts potency toward malignant transformation of OLP as well as a prognostic marker of OSCC. CD146 expression may be the cause of OLP progression into OSCC.

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“…25,26 Fibrin gels encapsulating chondrocytes, bone marrow-derived mesenchymal stem cells (BMSCs), fibroblasts, and osteoblasts have demonstrated that these biomaterials promote cellular adhesion and support proliferation, growth, and respective tissue differentiation. [27][28][29][30][31][32][33] Our own work utilized fibrin in combination with hyaluronic acid as a hybrid gel matrix for cartilage and bone TE. 34,35 In TE, many cell sources are considered to provide progenitor or mesenchymal stem cells required for regeneration.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Autologously Derived Biomaterials and Stem Cells for Bone Tissue Engineering

Mikael

Golebiowska

Kumbar

et al. 2020

Tissue Engineering Part A

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Despite progress, clinical translation of tissue engineering (TE) products/technologies is limited. A significant effort is underway to develop biomaterials and cells through a minimally modified process for clinical translation of TE products. Recently, bone marrow aspirate (BMA) was identified as an autologous source of cells for TE applications and is currently being tested in clinical therapies, but the isolation methods need improvement to avoid potential for contamination and increase progenitor cell yield. To address these issues, we reproducibly processed human peripheral blood (PB) and BMA to develop autologously derived biomaterials and cells. We demonstrated PB-derived biomaterial/gel cross-linking and fibrin gel formation with varied gelation times as well as biocompatibility through support of human bone marrow-derived stem cell survival and growth in vitro. Next, we established a plastic culture-free process that concentrates and increases the yield of CD146 + /CD271 + early mesenchymal progenitor cells in BMA (concentrated BMA [cBMA]). cBMA exhibited increased colony formation and multipotency (including chondrogenic differentiation) in vitro compared with standard BMA. PB-derived gels encapsulated with cBMA also demonstrated increased cell proliferation and enhanced mineralization when assessed for bone TE in vitro. This strategy can potentially be developed for use in any tissue regeneration application; however, bone regeneration was used as a test bed for this study.

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Healing capacity of bone marrow mesenchymal stem cells versus platelet-rich fibrin in tibial bone defects of albino rats: an in vivo study

Cited by 8 publications

References 28 publications

Biocompatibility assessment of different root-end filling materials implanted subcutaneously in rats: An in vivo study

Biocompatibility assessment of different root-end filling materials implanted subcutaneously in rats: An in vivo study

Evaluation of the Regenerative Effect of Rabbit BMMSCs and ADSCs. An In Vivo Study

Evaluation of Autologously Derived Biomaterials and Stem Cells for Bone Tissue Engineering

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