HE4 as a biomarker for diagnosis of lung cancer

He, Yongpeng; Li, Lixian; Tang, Jia-Xi; Lin, Yi; Zhao, Yi; Zhang, Haiwei; Wu, Zhijuan; Lei, Haike; Yu, Huiqing; Nian, Weiqi; Gan, Lin

doi:10.1097/md.0000000000017198

Cited by 15 publications

(17 citation statements)

References 27 publications

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“…[28][29][30][31] Additionally, some of them has been identi ed as biomarkers of diagnosis of lung cancer. [32][33][34] Here, the members of WFDC family were selected to do further comprehensive analysis of their roles in lung cancer. Our results demonstrated that WFDC1 was attenuated while WFDC2 was signi cantly elevated in carcinoma tissue.…”

Section: Wfdc21p Increases In Lung Carcinoma Cells and Tissuesmentioning

confidence: 99%

miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation

Zhang

Yan

et al. 2020

Preprint

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Background: Emerging evidence shows that lncRNA WFDC21P could promote STAT3 phosphorylation and microRNA 4293 SNP is associated with the risk of carcinomas, but the oncogenic functions of WFDC21P and miR-4293 in lung carcinoma are unclear.Methods: mRNA sequencing of lung carcinoma and control para-carcinoma tissues was performed to screen the potential targets. WFDC21P and miR-4293 levels were evaluated in lung carcinoma cells and tissues by qRT-PCR. The function of WFDC21P and miR-4293 on proliferation, apoptosis and metastasis were assessed by MTT, FACS, western blot, transwell assays, colony formation assays and xenografts experiment. RNA immunoprecipitation assays were implemented to verify the relationship between WFDC21P and mRNA-decapping enzyme 2 (DCP2). Furthermore, gain/loss of miR-4293 functions were used to determine its targeting relationship of DCP2. Results: WFDC21P expression is markedly enhanced in lung carcinoma tissue and cells. Moreover, WFDC21P promotes tumor cell proliferation and metastasis but suppresses apoptosis. Mechanistic investigations identified DCP2 can directly bind to WFDC21P and down-regulates its expression. DCP2 as a direct target of miR-4293 and its expression is suppressed by miR-4293. Consequently, miR-4293 can further promote WFDC21P expression by regulating DCP2. With positive correlation to WFDC21P expression, miR-4293 also plays oncogenic role in lung carcinoma. Furthermore, knockdown of WFDC21P results in functional attenuation of miR-4293 on tumor promotion. In vivo xenograft growth is also promoted by both WFDC21P and miR-4293. Conclusion: Our results establish oncogenic roles for both WFDC21P and miR-4293, and demonstrate that interactions between miRNAs and lncRNAs through DCP2 are important in lung carcinoma pathogenesis.

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Section: Wfdc21p Increases In Lung Carcinoma Cells and Tissuesmentioning

confidence: 99%

miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation

Zhang

Yan

et al. 2020

Preprint

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“…It has been proposed as a proteinase inhibitor with utility in the pulmonary immune system and the sperm maturation process, and it is expressed at low concentrations in various healthy tissues, including the respiratory and reproductive epithelium (27). HE4 is overexpressed in most subtypes of ovarian epithelial cancer (28) and some adenocarcinomas such as lung, endometrium and breast (29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of HE4 and CA125 as prognosis biomarkers during neoadjuvant chemotherapy in advanced epithelial ovarian cancer

Alegría-Baños

Jiménez-López

Vergara-Castañeda³

et al. 2021

Preprint

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Background Ovarian cancer (OC) is considered the most lethal gynecological cancer, of which more than 65% cases are diagnosed in advanced stages, requiring platinum-based neoadjuvant chemotherapy (NACT). Methods A prospective-longitudinal study was conducted among women with advanced epithelial ovarian cancer (AEOC), III and IV stages, and treated with NACT, at the National Cancer Institute – Mexico, from July 2017 to July 2018. Serum samples were obtained for quantification of CA125 and HE4 using ELISA at the first and in each of the three NACT cycles. The therapeutic response was evaluated through standard tomography. We determined whether CA125 and HE4, alone or in combination, were associated with TR to NACT during follow up. Results 53 patients aged 38 to 79 years were included, 92.4% presented papillary serous subtype OC. Higher serum HE4 levels were observed in patients with non-tomographic response (6.89 vs 5.19 pmol/mL; p = 0.031), specially during the second (p = 0.039) and third cycle of NACT (p = 0.031). Multivariate-adjusted models showed an association between HE4 levels and TR, from the second treatment cycle (p = 0.042) to the third cycle (p = 0.033). Changes from baseline HE4 levels during the first cycle was negative associated with TR. No associations were found between CA125 and TR. Conclusions Serum HE4 levels were independently associated with TR among patients with AOEC treated with NACT, also a reduction between baseline HE4 and first chemotherapy levels was also independently associated with the TR. These findings might be relevant for predicting a lack of response to treatment.

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“…Molecular biomarkers are usually evaluated for their individual associations with the disease risks and have been widely used in the decision processes of disease diagnosis and prognosis (He et al, 2019;Leiro et al, 2019;Yokobori et al, 2019;Muller et al, 2020). Transcription factor (TF) plays a critical role in cancer cell processes, such as cell proliferation, apoptosis, migration, and regulate gene expression.…”

Section: Introductionmentioning

confidence: 99%

Detection and Independent Validation of Model-Based Quantitative Transcriptional Regulation Relationships Altered in Lung Cancers

Duan

Song

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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Differential expressions of genes are widely evaluated for the diagnosis and prognosis correlations with diseases. But limited studies investigate how transcriptional regulations are quantitatively altered in diseases. This study proposes a novel model-based quantitative measurement of transcriptional regulatory relationships between mRNA genes and Transcription Factor (TF) genes (mqTrans features). This study didn't consider the regulatory relationships between TF genes, so the mRNA genes were the protein-coding genes excluding the TF genes. The models are trained in the control samples in a lung cancer dataset and evaluated in two independent datasets and the hold-out testing samples from the third dataset. Twenty-nine mRNA genes are detected with transcriptional regulations quantitatively altered in lung cancers. The transcriptional modification technologies like RNA interference (RNAi) may be utilized to restore the altered transcriptional regulations in lung cancers.

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HE4 as a biomarker for diagnosis of lung cancer

Abstract: Supplemental Digital Content is available in the text

Cited by 15 publications

References 27 publications

miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation

miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation

Kinetics of HE4 and CA125 as prognosis biomarkers during neoadjuvant chemotherapy in advanced epithelial ovarian cancer

Detection and Independent Validation of Model-Based Quantitative Transcriptional Regulation Relationships Altered in Lung Cancers

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