HDL subfraction distribution of paraoxonase-1 and its relevance to enzyme activity and resistance to oxidative stress

Moren, Xenia; Deakin, Sara; Liu, Ming-Lin; Taskinen, Marja‐Riitta; James, Richard

doi:10.1194/jlr.m700439-jlr200

Cited by 35 publications

(22 citation statements)

References 32 publications

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“…On the other hand, approximately half of HDL particles contain apoA-II (Wroblewska et al 2009). In addition to apoA-II, the two subclasses may differ in their content of other proteins, as exemplified by PON1 which preferably associates with LpA-I (Moren et al 2008). LpA-I particles can be further subfractionated according to size.…”

Section: Heterogeneity In Hdl Proteinsmentioning

confidence: 99%

Structure of HDL: Particle Subclasses and Molecular Components

Kontush

Lindahl

Lhomme

et al. 2014

Handbook of Experimental Pharmacology

219

226

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Section: Heterogeneity In Hdl Proteinsmentioning

confidence: 99%

Structure of HDL: Particle Subclasses and Molecular Components

Kontush

Lindahl

Lhomme

et al. 2014

Handbook of Experimental Pharmacology

219

226

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“…Majority of this enzyme of which almost all of it is associated with HDL cholesterol in humans, is produced in liver (39,40). It is found that serum PON 1 levels in patients with CVD were lower than the normal control group.…”

Section: Discussionmentioning

confidence: 90%

Is there a relationship between serum paraoxonase level and epicardial fat tissue thickness?

Ertem¹,

Erayman²,

Efe³

et al. 2014

Anadolu Kardiyol Derg

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Objective: This study aimed to show the relationship between serum paraoxonase 1 level and the epicardial fat tissue thickness. Methods: Two hundred and seven patients without any atherosclerotic disease history were included in this cross-sectional observational study. Correlation analysis was performed to determine the correlation between epicardial fat tissue thickness, which was measured by echocardiography and serum paraoxonase 1 level. Also correlation analysis was performed to show correlation between patients' clinical and laboratory findings and the level of serum paraoxonase 1 (PON 1) and the epicardial fat tissue thickness. Pearson and Spearman test were used for correlation analysis. Results: No linear correlation between epicardial fat tissue thickness and serum PON 1 found (correlation coefficient: -0.127, p=0.069). When epicardial fat tissue thickness were grouped as 7 mm and over, and below, and 5 mm and over, and below, serum PON 1 level were significantly lower in ≥7 mm group (PON1 : 168.9 U/L) than <7 mm group (PON 1: 253.9 U/L) (p<0.001). Also hypertension prevalence was increased in ≥7 mm group (p=0.001). Serum triglyceride was found to be higher in ≥7 mm group (p=0.014), body mass index was found higher in ≥5 mm group (p=0.006). Conclusion: Serum PON 1level is not correlated with the epicardial fat tissue thickness. But PON 1 level is lower in patients with epicardial fat tissue thickness 7 mm and over. Therefore, increased atherosclerosis progression can be found among patients with 7 mm and higher epicardial fat tissue thickness. (Anadolu Kardiyol Derg 2014; 14: 115-20)

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“…These results have clear implications for analyzing the meaningful biological function(s) of any PON1 therapeutic testing, as such a complex formation is well known to be a pre-requisite for maintaining stable PON1 activity in serum. 34,35 In addition, these results show that adenoviral gene transfer results in functional PON1-HDL complexes, without resorting to extraordinary genetic engineering means to generate such complexes. 32 A study which compared the effectiveness of pre-treatment with recombinant chimeric PON1 to pre-treatment with a PON1-HDL complex found that the recombinant chimeric PON1 Abbreviations: Ad-hPON1, adenovirus-expressing hPON1; hPON1, human paraoxonase1.…”

Section: Adenovirusmentioning

confidence: 85%

Adenovirus-mediated human paraoxonase1 gene transfer to provide protection against the toxicity of the organophosphorus pesticide toxicant diazoxon

et al. 2010

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Human paraoxonase1 (hPON1) is a potential therapeutic against the toxicity of organophosphorus (OP) pesticides and chemical warfare nerve agents. We tested whether PON1 gene transfer using adenovirus provides protection against the toxicity of the OP diazoxon. Using an adenovirus construct containing hPON1 gene, we showed elevated levels of recombinant hPON1 in vitro in 293A cells and in vivo in mice. The recombinant enzyme was secreted by 293A cells into culture medium and into the systemic circulation of mice. Western blotting revealed that the virally expressed hPON1 had the expected molecular weight of 45 kDa. Recombinant hPON1 in mice was in complex with mouse high-density lipoprotein (HDL) and migrated more slowly than endogenous hPON1 in the human HDL complex. Mice injected with adenovirus expressed PON1 at 600-3480 U ml -1 on day 5 post-treatment, which is 8-50-fold above endogenous. Six mice expressing hPON1 survived 2LD 50 doses of diazoxon. Four of the six mice survived a second dose of diazoxon (for a total of 4LD 50 ) administered 24 h later. In contrast, none of the three mice in the control group survived one 2LD 50 dose. These results show that hPON1 in mice functions as a prophylactic and offers significant protection against lethal doses of diazoxon.

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HDL subfraction distribution of paraoxonase-1 and its relevance to enzyme activity and resistance to oxidative stress

Cited by 35 publications

References 32 publications

Structure of HDL: Particle Subclasses and Molecular Components

Structure of HDL: Particle Subclasses and Molecular Components

Is there a relationship between serum paraoxonase level and epicardial fat tissue thickness?

Adenovirus-mediated human paraoxonase1 gene transfer to provide protection against the toxicity of the organophosphorus pesticide toxicant diazoxon

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