Has_circ_0055625 from circRNA profile increases colon cancer cell growth by sponging miR‐106b‐5p

Zhang, Jian; Liu, Hua; Zhao, Ping; Zhou, Hao; Mao, Tao

doi:10.1002/jcb.27355

Cited by 42 publications

(25 citation statements)

References 19 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, many circRNAs have been reported as biomarkers for tumor diagnosis or therapy [26, 27]. For example, by studying more than 100 gastric cancer tissues with paired adjacent no tumorous tissues and plasma, hsa-circ-002059 was found to be down-regulated in gastric cancer tissues and plasma [28].…”

Section: Discussionmentioning

confidence: 99%

Potential Role of circPVT1 as a proliferative factor and treatment target in esophageal carcinoma

Zhong

Chen

et al. 2019

Cancer Cell Int

View full text Add to dashboard Cite

Background Many circRNAs have been reported to play important roles in cancer development and have the potential to serve as a novel class of biomarkers for clinical diagnosis. However, the role of circRNAs in esophageal carcinoma (EC) remains unclear. In the current study, we investigated the potential role of circPVT1 in esophageal carcinoma. Methods Quantitative real-time PCR was performed to detect circPVT1 levels. CircPVT1-specific siRNA or plasmids were used to knock down or overexpression the target RNA. Hoechst Staining was implemented to evaluate the ratio of cell apoptosis. Transwell migration assays were carried out to study the effects of circPVT1 on esophageal squamous cell carcinoma cell invasion. RegRNA 2.0 was used for bioinformatics analysis. The expression levels of Pax-4, Pax-6, PPARα and PPAR-γ were assessed using Western blot. Results In the present study, we demonstrated a significant up-regulation of circPVT1 levels in EC tissues and cancer cell lines. The levels of circPVT1 decreased significantly when the cells were maintained to over-confluence. These results suggested a potential role for circPVT1 in cell proliferation. In addition, overexpressing circPVT1 in TE-10 cell promoted invasive ability of cancer cell. In contrast, siRNA knockdown of circPVT1 inhibited this phenomenon, leading to increased apoptosis levels of TE-10 cell. What’s more, miR-4663 had the effect of inhibiting tumor growth by downregulated Paxs and upregulated PPARs. Whereas, after the addition of circPVT1, this effect no longer worked, suggesting that circPVT1 may affect the malignancy of the tumor by affecting miRNA and regulating the levels of Paxs and PPARs. Conclusions Collectively, our study reveals a critical role for circPVT1 in esophageal carcinoma, which may provide new insights of this circRNA as a biomarker for the diagnosis and treatment target of EC.

show abstract

Section: Discussionmentioning

confidence: 99%

Potential Role of circPVT1 as a proliferative factor and treatment target in esophageal carcinoma

Zhong

Chen

et al. 2019

Cancer Cell Int

View full text Add to dashboard Cite

show abstract

“…Circ_0055625 was upregulated in CC to promote tumor growth and invasion via circ_0055625-miR-106b-5p-integrin β8 (ITGB8) axis. ITGB8 is a member of the integrin family, which binds through its α subunit and plays major roles in regulating cell invasion [54]. ITGB8 was targeted through increased expression of hsa_circ_0046701 sponging miR-142-3p to promote glioma progression [55].…”

Section: Altered Tumor Cell Adhesion To Ecm Proteinsmentioning

confidence: 99%

The crosstalk between circular RNAs and the tumor microenvironment in cancer metastasis

Shao

Lü

2020

Cancer Cell Int

View full text Add to dashboard Cite

Background Carcinomas are highly heterogeneous with regard to various cancer cells within a tumor microenvironment (TME), which is composed of stromal cells, blood vessels, immunocytes, and modified extracellular matrix. Focus of the study Circular RNAs (circRNAs) are non-coding RNAs that are expressed in cancer and stromal cells. They are closely associated with cancer metastasis as their expression in tumor cells directs the latter to migrate to different organs. circRNAs packaged in exosomes might be involved in this process. This is particularly important as the TME acts in tandem with cancer cells to enhance their proliferation and metastatic capability. In this review, we focus on recent studies on the crosstalk between circRNAs and the TME during cancer metastasis. Conclusion We particularly emphasize the roles of the interaction between circRNAs and the TME in anoikis resistance, vessel co-option, and local circRNA expression in directing homing of exosome.

show abstract

“…For the selected examples, circRNA_33287 worked as a key regulator in osteogenic differentiation via sponging miR-214-3p [28]. Likewise, circ_0055625 plays a significant role in promoting CRC cells growth and metastasis by functioning as a miRNA sponge for miR-106b-5p [29]. In the current study, miR-183, a recognized oncogene [24], was found to be a downstream gene of circ_0026344 since it was negatively regulated by circ_0026344.…”

Section: Discussionmentioning

confidence: 58%

Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183

Shen

Cheng

Liu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Background: CircRNA circ_0026344 was previously revealed as a tumour-suppressive gene in colorectal cancer (CRC) progression. The purpose of this research was to investigate the role of circ_0026344 in CRC cells metastasis induced by chemokines. Methods: Two human CRC cell lines SW480 and Caco-2 were treated by CCL20 and CXCL8. Cell proliferation, migration/invasion, expression of epithelial-mesenchymal transition (EMT) inducers and the expression of circ_0026344 were measured using sulforhodamine B assay, Transwell chamber, western blot and qRT-PCR, respectively. The effects of circ_0026344 on CRC cells migration/invasion and the expression of EMT inducers were evaluated. Moreover, the downstream miRNA and signalling pathways of circ_0026344 were studied. Results: CCL20 and CXCL8 synergized to facilitate the proliferation, migration and invasion of CRC cells. At the meantime, E-cadherin was downregulated, whereas N-cadherin, Vimentin and Snail were upregulated by CCL20 and CXCL8 co-stimulation, which was accompanied by the mobilization of PI3K/ AKT/ERK signalling. More interestingly, the expression of circ_0026344 was down-regulated by CCL20 and CXCL8 co-stimulation. Silence of circ_0026344 increased the migratory and invasive capacities of CRC cells and increased EMT process as well. Overexpression of circ_0026344 led to a contrary impact. miR-183 was negatively regulated by circ_0026344, and the inhibitory effects of circ_0026344 overexpression on Wnt/b-catenin pathway were reversed when miR-183 was overexpressed. Conclusion: Overexpression of circ_0026344 restrained CRC metastasis and EMT induced by CCL20 and CXCL8 synergistical treatment. miR-183 was a downstream effector of circ_0026344, and the antitumour function of circ_0026344 might be involved in the repressed Wnt/b-catenin signalling. HIGHLIGHTS 1. CCL20 and CXCL8 synergize to decrease the expression of circ_0026344; 2. Silence of circ_0026344 promotes CRC cells migration, invasion and EMT process; 3. miR-183 is a downstream effector of circ_0026344.

show abstract

Has_circ_0055625 from circRNA profile increases colon cancer cell growth by sponging miR‐106b‐5p

Cited by 42 publications

References 19 publications

Potential Role of circPVT1 as a proliferative factor and treatment target in esophageal carcinoma

Potential Role of circPVT1 as a proliferative factor and treatment target in esophageal carcinoma

The crosstalk between circular RNAs and the tumor microenvironment in cancer metastasis

Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183

Contact Info

Product

Resources

About