Harvesting Technique Affects Adipose-Derived Stem Cell Yield

Iyyanki, Tejaswi; Hubenak, Justin; Li, Jun; Chang, Edward I.; Beahm, Elisabeth K.; Zhang, Qixu

doi:10.1093/asj/sju055

Cited by 55 publications

(40 citation statements)

References 46 publications

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“…The present study indicates that gentle water-jet-assisted liposuction is similar to the commonly used Coleman’s method in terms of SVF and ASC recovery. Compared to direct fat excision method, liposuction performed using the Coleman’s method yield less ASCs, while power-assisted liposuction has been shown to yield same numbers of ASCs with similar characteristics ( 18 , 19 ). Another study has reported that contrary to fat resection, manual liposuction preserves the microvascular network ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

Adipose Mesenchymal Stem Cells Isolated after Manual or Water-jet-Assisted Liposuction Display Similar Properties

et al. 2016

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Mesenchymal stem or stromal cells (MSC) are under investigation in many clinical trials for their therapeutic potential in a variety of diseases, including autoimmune and inflammatory disorders. One of the main sources of MSCs is the adipose tissue, which is mainly obtained by manual liposuction using a cannula linked to a syringe. However, in the past years, a number of devices for fat liposuction intended for clinical use have been commercialized but few papers have compared these procedures in terms of stromal vascular fraction (SVF) or adipose mesenchymal stromal cells (ASC). The objective of the present study was to compare and qualify for clinical use the ASC obtained from fat isolated with the manual or the Bodyjet® water-jet-assisted procedure. Although the initial number of cells obtained after collagenase digestion was higher with the manual procedure, the percentage of dead cells, the number of colony forming unit-fibroblast and the phenotype of cells were identical in the SVF at isolation (day 0) and in the ASC populations at day 14. We also showed that the osteogenic and adipogenic differentiation potentials of ASCs were identical between preparations while a slight but significant higher in vitro immunosuppressive effect was observed with ASCs isolated from fat removed with a cannula. The difference in the immunomodulatory effect between ASC populations was, however, not observed in vivo using the delayed-type hypersensitivity (DTH) model. Our data, therefore, indicate that the procedure for fat liposuction does not impact the characteristics or the therapeutic function of ASCs.

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Section: Discussionmentioning

confidence: 99%

Adipose Mesenchymal Stem Cells Isolated after Manual or Water-jet-Assisted Liposuction Display Similar Properties

et al. 2016

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“…Adipose harvested through direct excision or with the Coleman technique with centrifugation has been shown to yield the most SVF cells and ASCs. 15 The SVF is a heterogenous population of cells and rich source of ASCs, preadipocytes, vascular progenitors, fibroblasts, pericytes, macrophages, mast cells, and lymphocytes. 16 Culture of SVF on a tissue culture surface with growth media yields an adherent and more homogenous population of ASCs that can be expanded in vitro and differentiated into cells of ectodermal and endodermal lineages, including adipocytes, osteoblasts, chondrocytes, endothelial cells, neuron-like cells, myocytes, and cardiomyocytes.…”

Section: Does Fat Grafting Transfer Viable Stem Cells?mentioning

confidence: 99%

Do Stem Cells Have an Effect When We Fat Graft?

Rinker

2016

Annals of Plastic Surgery

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Fat grafting has become a widely accepted modality of soft tissue restoration and has found applications in many areas of aesthetic and reconstructive plastic surgery. Numerous claims have been made regarding the regenerative effects of fat grafting on the recipient bed. The purpose of this paper is to survey the available literature to answer the question of whether fat grafting has a positive effect on the surrounding tissues. It has been convincingly demonstrated that fat grafts contain viable adipose-derived stem cells (ASCs). The fate of these cells is determined by the microenvironment of the recipient bed, but animal studies have shown that a large fraction of ASCs survive engraftment. Numerous clinical studies have demonstrated the positive effects of fat grafting on recipient tissues. Improvement in validated scar scores as well as scar stiffness measurements have been documented after fat grafting of burn scars. Fat grafting has also been convincingly demonstrated to improve the quality of irradiated tissues, as measured by validated clinical scales and staged histology. It is ultimately unclear whether ASCs are responsible for these effects, but the circumstantial evidence is weighty. Fat grafting is effective for volumizing and improving skin quality in the setting of radiation, burns, and other scars. The observed effects are likely due to ASCs, but the evidence does not support the routine use of ASC-enriched fat grafts.

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“…However, in other studies, the anatomical site did have an influence on the total SVF and on the ADSC yields. Iyyanki et al observed significantly higher total SVF yields from the abdominal harvesting site than from the flank and axilla harvesting sites; however, the ADSC yields did not differ significantly [18]. In a study by Fraser et al, the abdomenadipocyte yield was 1.7-fold higher than the hip-adipocyte yield, and the adipocyte yields displayed large donor-todonor variabilities [22].…”

Section: Discussionmentioning

confidence: 97%

“…Not only the negative pressure during liposuction and in the donor harvesting site but also different harvesting procedures [18] and other individual donor factors have been found to influence the viability, proliferation, and differentiation characteristics of ADSCs. Further factors include body mass index (BMI), age, gender, intercurrent diseases, such as diabetes mellitus, and also radiotherapy and drug treatment [19].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates

Trávníčková

Pajorová

Zárubová

et al. 2020

Stem Cells International

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Background. Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs. Methods. We studied the influence of the harvesting site, i.e., inner thigh (n=3), outer thigh (n=7), and abdomen (n=9), and of negative pressure, i.e., low (-200 mmHg) and high (-700 mmHg), on the characteristics of isolated ADSCs. We counted initial yields of attached cells after isolation. In subsequent passage, we studied the number, viability, diameter, doubling time, mitochondrial activity, and CD surface markers of isolated ADSCs. Results. We revealed higher initial cell yields from the outer thigh region than from the abdomen region. Negative pressure did not influence the cell yields from the outer thigh region, whereas the yields from the abdomen region were higher under high negative pressure than under low negative pressure. In the subsequent passage, in general, no significant relationship was identified between the different negative pressure and ADSC characteristics. No significant difference was observed in the characteristics of thigh ADSCs and abdomen ADSCs. Only on day 1, the diameter was significantly bigger in outer thigh ADSCs than in abdomen ADSCs. Moreover, we noted a tendency of thigh ADSCs (i.e., inner thigh+outer thigh) to reach a higher cell number on day 7. Discussion. The harvesting site and negative pressure can potentially influence initial cell yields from lipoaspirates. However, for subsequent in vitro culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics.

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Harvesting Technique Affects Adipose-Derived Stem Cell Yield

Abstract: Adipose tissue harvested from the abdomen through direct excision or Coleman's technique with centrifugation was found to yield the most SVF cells and ASCs.

Cited by 55 publications

References 46 publications

Adipose Mesenchymal Stem Cells Isolated after Manual or Water-jet-Assisted Liposuction Display Similar Properties

Adipose Mesenchymal Stem Cells Isolated after Manual or Water-jet-Assisted Liposuction Display Similar Properties

Do Stem Cells Have an Effect When We Fat Graft?

The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates

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