Harnessing the potential of hairy roots: dawn of a new era

Guillon, Stéphanie; Trémouillaux-Guiller, Jocelyne; Pati, Pratap Kumar; Rideau, Marc; Gantet, Pascal

doi:10.1016/j.tibtech.2006.07.002

Cited by 188 publications

(104 citation statements)

References 54 publications

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“…In addition to the strategy of the manipulation of the developmental regulators for the spatial expansion of metabolite-producing areas, tissue culture techniques, such as cultures of multiple shoots (Miura et al 1988), adventitious roots (Kusakari et al 2000;Aoyagi et al 2001;Yokoyama et al 2003), hairy roots (Shanks and Morgan 1999;Seki et al 2005;Guillon et al 2006) and intact tissues (Iwase et al 2005;Dörnenburg et al 2008), would be an effective strategy, in combination with the manipulation of regulators that control metabolic pathways, to obtain higher productivity of useful metabolites. Moreover, both the availability of chemicals for promotion of biosynthesis, such as elicitors, and the utility of gene-inducible systems are also advantages of tissue culture technology for the efficient production of useful metabolites.…”

Section: Manipulation Of Developmental Regulators For the Control Of mentioning

confidence: 99%

Manipulation of plant metabolic pathways by transcription factors

Iwase

Matsui

Ohme‐Takagi

2009

Plant Biotechnology

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Using inexhaustible light energy, plants produce large numbers of metabolites, which include not only primary metabolites but also secondary metabolites, through various metabolic pathways. Secondary metabolites play integral roles in growth, development, symbiosis, reproduction and tolerance to biotic and abiotic stresses in plants (Kutchan 2001). More than 200,000 different metabolites are estimated to be produced in the plant kingdom (Dixon and Strack 2003; Trethewey 2004). On the other hand, the number of metabolites that prokaryotes and animals produce is estimated to be 5000 to 25000, which is much less than that of plants (Trethewey 2004). For more than thousands years, mankind has been utilizing plant metabolites as foods, pharmaceutical compounds and raw materials for industry (Oksman-Caldentey and Saito 2005). In addition, mankind uses plant metabolites for relaxation, e.g. flower color and fragrance. Therefore, plant biosynthetic pathways have been intensively studied, and genetic engineering to control the biosynthetic pathways has been applied for the effective production of useful metabolites (Capell and Christou 2004;Dixon 2005).As well as metabolites, it is likely that plants have many, and various, families of transcription factors compared with other organisms (Riechmann et al. 2000). Transcription factors are defined as proteins that recognize a specific DNA sequence on the promoter region of genes and regulate the expression of the genes positively (activator) or negatively (repressor). Each plant metabolic pathway consists of multiple enzymatic steps, and each enzyme gene is under the regulation of transcription factors ( Figure 1A). Therefore, the diversity of plant transcription factors seems to be tightly linked to the diversity of plant metabolites. This linkage might be a consequence of evolution, which is responsible for their sessile lifestyle and interactions with various environmental stresses (Grotewold 2005).It has become evident that a number of transcription factors act as master regulators of various plant functions, and several of them have been identified to be key regulators for metabolic pathways (Broun 2004;Grotewold 2008). These transcription factors (referred to as metabolic regulators) activate or repress the expression of enzyme genes in specific metabolic pathways. Manipulations of such transcription factors appear to be more effective for the control of metabolic pathways than that of single enzyme genes in plants (Braun et al. 2001;Capell and Christou 2004), because plant metabolic pathways are composed of multiple and complicated steps, and various enzymes are involved at each step. A transcription factor often regulates the expression of multiple genes involved in a biosynthetic Abstract Plant metabolites are produced through complex processes that include multiple enzymatic steps, branched pathways and regulation by a number of functionally redundant transcription factors. In addition, plants synthesize and accumulate each metabolite, especially secondary metabol...

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Section: Manipulation Of Developmental Regulators For the Control Of mentioning

confidence: 99%

Manipulation of plant metabolic pathways by transcription factors

Iwase

Matsui

Ohme‐Takagi

2009

Plant Biotechnology

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“…The greatest advantage of hairy roots is the ability to produce higher levels of secondary metabolites as compared to intact plants [11]. Medicinal plants have been widely explored for potential hairy root culture and production of their bioactive compounds [12].…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Torkamani

Jafari²,

Abbaspour

et al. 2014

Open Life Sciences

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Valerenic acid (VA) is a pharmacologically-active sesquiterpene found in valerian (Valeriana officinalis L., Valerianaceae) roots and rhizomes. The plant produces only small amounts of this metabolite naturally. So, induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, Wild-type strain ‘A13’ of Agrobacterium rhizogenes was used to induce hairy roots in valerian. The influence of three different elicitors including Fusarium graminearum extract (FE), methyl jasmonate (MJ) and salicylic acid (SA) on VA production in the selected hairy root line ‘LeVa-C4’ was also investigated. The 23-day-old cultures were treated with different concentrations of the elicitors at exposure time of 3 and 7 days. FE (1%) and MJ (100 µM L−1) highly promoted VA production at 7 days after elicitation, to a level of 12.31- and 6-fold higher than that of non-elicited controls, respectively, and FE did not exert any negative effects on biomass yield of hairy root. SA did not significantly increase the production of VA. This is the first time study to assess the elicitation of hairy root cultures to promote VA biosynthesis in valerian and the resulting experiments demonstrated that F. graminearum extract and MJ were indeed a potent inducer of VA biosynthesis.

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“…Hundreds of species have been transformed with A. rhizogenes with the aim of achieving transformed root induction. The prospects for the production of medicinal compounds in transformed roots were previously reviewed (Georgiev et al 2007;Guillon et al 2006;Uozumi 2004). Several Boraginaceous species have been also transformed with A. rhizogenes for the production of shikonin derivatives.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Sykłowska-Baranek

Pietrosiuk

Gawron

et al. 2011

Plant Cell Tiss Organ Cult

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The effects of medium exchange and methyl jasmonate addition on growth and production of shikonin and its derivatives acetylshikonin and isobutyrylshikonin in hairy root cultures of Lithospermum canescens were investigated. Responses varied depending on the transgenic line and stage of growth at which these lines were subjected to treatment. Shikonin itself was not detected, irrespective of the transgenic line and culture treatment used. A eightfold increase in acetylshikonin and isobutyrylshikonin accumulation was achieved when 32-day-old transgenic roots of Lc1D line were transferred from LS to M9 medium for the subsequent 3 weeks of culture. Methyl jasmonate exerted a detrimental effect on red naphthoquinones production. The extracts obtained from roots cultivated in M9 medium for 3 weeks were subjected to a cytotoxicity assay and displayed cytotoxic activity against human promyelocytic leukemia cells (HL-60) at the dose of 4 lg ml -1 .

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Harnessing the potential of hairy roots: dawn of a new era

Cited by 188 publications

References 54 publications

Manipulation of plant metabolic pathways by transcription factors

Manipulation of plant metabolic pathways by transcription factors

Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

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