Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Abstract

Valerenic acid (VA) is a pharmacologically-active sesquiterpene found in valerian (Valeriana officinalis L., Valerianaceae) roots and rhizomes. The plant produces only small amounts of this metabolite naturally. So, induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, Wild-type strain ‘A13’ of Agrobacterium rhizogenes was used to induce hairy roots in valerian. The influence of three different elicitors including Fusarium graminearum extract (FE), methyl jasmonate (MJ) and salicylic acid (SA) on VA production in the selected hairy root line ‘LeVa-C4’ was also investigated. The 23-day-old cultures were treated with different concentrations of the elicitors at exposure time of 3 and 7 days. FE (1%) and MJ (100 µM L−1) highly promoted VA production at 7 days after elicitation, to a level of 12.31- and 6-fold higher than that of non-elicited controls, respectively, and FE did not exert any negative effects on biomass yield of hairy root. SA did not significantly increase the production of VA. This is the first time study to assess the elicitation of hairy root cultures to promote VA biosynthesis in valerian and the resulting experiments demonstrated that F. graminearum extract and MJ were indeed a potent inducer of VA biosynthesis.

Effect of Elicitors on Morpho-Physiological Performance and Metabolites Enrichment in Valeriana jatamansi Cultivated Under Aeroponic Conditions

“…(2012) observed a threefold increase in valtrate content using 100 mg/L MJ treatment, in the adventitious root culture of V. amurensis. Similarly, Torkamani et al. (2014) reported increased valerenic acid production (two times higher) in V. officinalis hairy root culture with MJ (100 μM) treatment.…”

“…Cui et al (2012) observed a threefold increase in valtrate content using 100 mg/L MJ treatment, in the adventitious root culture of V. amurensis. Similarly, Torkamani et al (2014) reported increased valerenic acid production (two times higher) in V. officinalis hairy root culture with MJ (100 mM) treatment. In the present investigation, elicitation resulted in a significant increase of VA, HVA, and AVA content, not only in the root (1.78, 7.89, and 2.38 mg/g DW) but most prominently in the leaf (2.47, 8.37 and 1.02 mg/g DW), for the first time.…”

“…Elicitors, such as methyl jasmonate and yeast extract, act as signaling molecules, recognized by elicitor‐specific receptors on the plant cell membrane, and induce transcriptional activation of genes involved in the biosynthesis of secondary metabolites ( Cui et al., 2012 ; Torkamani et al., 2014 ; Halder et al., 2019 ). However, for efficient and effective elicitation processes, optimization of different parameters, such as type, concentration, exposure duration, and treatment schedule of elicitors are the foremost requirements ( Halder et al., 2019 ), but to date no report is published on the elicitors (such as methyl jasmonate and yeast extract) mediated enhancement of plant growth and secondary metabolites content in aeroponically-cultivated V. jatamansi .…”

Mathematical Modeling of Growth and Paclitaxel Biosynthesis in Corylus avellana Cell Culture Responding to Fungal Elicitors Using Multilayer Perceptron-Genetic Algorithm

“…In vitro culture of hazel ( Corylus avellana , European filbert) has been made known as a promising and inexpensive strategy for producing paclitaxel ( Gallego et al, 2017 ; Salehi et al, 2017 ; Salehi et al, 2018c ; Salehi et al, 2019b ; Salehi et al, 2019c ; Farhadi et al, 2020 ; Salehi et al, 2020 ). Biosynthesizing bioactive compounds in plants is influenced by various factors ( Torkamani et al, 2014 ; Salehi et al, 2017 ; Salehi et al, 2018a ; Salehi et al, 2018b ; Salehi et al, 2018c ; Salehi et al, 2019a ; Salehi et al, 2019b ; Salehi et al, 2019c ; Salehi et al, 2019d ). Previous studies ( Salehi et al, 2019b ; Salehi et al, 2019c ; Farhadi et al, 2020 ; Salehi et al, 2020 ) demonstrated the positive influences of cell extract (CE) and culture filtrate (CF) of endophytic fungi on paclitaxel biosynthesis in cell suspension culture (CSC) of C. avellana .…”

Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion system and MeJA

“…Hook. f. [44], and induction of 100 µM MeJA for 7 days promoted the production of valerian acid in valerian hairy root cultures of Valeriana o cinalis [45]. Therefore, it is necessary to screen MeJA concentration and treatment time in order to obtain the maximum accumulation of bioactive compounds.…”