Harmine promotes osteoblast differentiation through bone morphogenetic protein signaling

Yonezawa, Takayuki; Lee, Jiwon; Hibino, Ayaka; Asai, Midori; Hojo, Hironori; Cha, Byung‐Yoon; Teruya, Toshiaki; Nagai, Kazuo; Chung, Ung‐il; Yagasaki, Kazumi; Woo, Je‐Tae

doi:10.1016/j.bbrc.2011.05.001

Cited by 68 publications

(50 citation statements)

References 20 publications

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“…Overexpression of osterix stimulated osteoblast differentiation of bone marrow stromal cells in the peri‐implant tissue, and increased the bone−implant contact of machine‐turned Ti implants in the mouse femur 37. There was no notable difference in expression level of early marker gene for osteoblast differentiation (BSP38) between two groups, however, mRNA expression of terminal marker gene for osteoblast differentiation (osteocalcin) was upregulated in the peri‐implant bone of the SLA/Sr implants than the SLActive implants (1.2‐fold; p < 0.05), indicating a more mature stage of osteoblasts in the peri‐implant bone of the SLA/Sr implants 39, 40. Thus, a notably upregulated expression of critical osteoblast‐specific transcription factor gene for osteoblast differentiation (osterix) resulted in a concomitant increase in the expression of osteocalcin gene in the peri‐implant bone tissue of the SLA/Sr implants.…”

Section: Resultsmentioning

confidence: 91%

Adherence to gastroprotection during cyclooxygenase 2 inhibitor treatment and the risk of upper gastrointestinal tract events: A population‐based study

Valkhoff¹,

Soest²,

Mazzaglia

et al. 2012

Arthritis & Rheumatism

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Objective. Guidelines recommend coprescription of gastroprotective agents (GPAs) in patients receiving cyclooxygenase 2 inhibitors (coxibs) who are at high risk of upper gastrointestinal (UGI) tract complications (i.e., patients with a previous complicated ulcer or with multiple risk factors). Suboptimal GPA adherence has been shown to diminish the gastroprotective effect during use of nonselective nonsteroidal antiinflammatory drugs, but little is known about the effect of GPA adherence during coxib treatment. We undertook this study to determine the association between GPA adherence and UGI tract events among patients receiving coxibs.Methods. Using primary care data from 3 databases, we conducted a case-control study in a cohort of patients age >50 years who were newly starting treatment with coxibs and concomitantly taking GPAs. Patients who had a UGI tract event (bleeding or symptomatic ulcer) were matched to event-free controls for age, sex, database, and calendar date. Coxib treatment intervals were defined as consecutive coxib prescriptions with intervening gaps not exceeding the duration of the previous coxib prescription. Adherence to GPAs was calculated as the proportion of days of coxib treatment covered by a GPA prescription. Odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated using conditional logistic regression analysis.Results. The coxib plus GPA-treated cohort consisted of 14,416 coxib-treated patients who received GPAs for at least 1 day, yielding 16,442 coxib treatment intervals in which a GPA was coprescribed. Most patients were treated with coxibs for <30 days. Seventyfour patients had a UGI tract event during or shortly after a coxib treatment interval in which a GPA was coprescribed, with an incidence rate of 11.9 (95% CI Study results were presented in part by Drs. Valkhoff and

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Section: Resultsmentioning

confidence: 91%

Adherence to gastroprotection during cyclooxygenase 2 inhibitor treatment and the risk of upper gastrointestinal tract events: A population‐based study

Valkhoff¹,

Soest²,

Mazzaglia

et al. 2012

Arthritis & Rheumatism

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“…Because osteocalcin is a strong indicator of osteoblast differentiation (Yonezawa et al, 2011), and temporal expression and maturation of extracellular matrix proteins such as fibronectin (Jimbo et al, 2007) or collagen type I (Franceschi and Iyer, 1992;Jimbo et al, 2010) are essential for mineralization, these results indicate that the rate of mineralization was enhanced for the test implants.…”

Section: Discussionmentioning

confidence: 90%

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

et al. 2011

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Nanostructured calcium phosphate (CaP) has been histologically and biomechanically proven to enhance osseointegration of implants; however, conventional techniques were not sufficiently sensitive to capture its biological effects fully. Here, we compared the conventional removal torque (RTQ) evaluation and gene expression in tissues around nanostructured CaP-coated implants, using real-time RT-PCR, with those of uncoated implants, in a rabbit model. At 2 wks, RTQ values were significantly higher, alkaline phosphatase (ALP) expression was significantly higher, and runt-related transcription factor 2 and tumor necrosis factor-α expressions were significantly lower in the coated than in the uncoated implants. This indicates that inflammatory responses were suppressed and osteoprogenitor activity increased around the CaP-coated surface. At 4 wks, although RTQ values did not significantly differ between the 2 groups, ALP and osteocalcin (OCN) were significantly up-regulated in the coated group, indicating progressive mineralization of the bone around the implant. Moreover, an osteoclast marker, adenosine triphosphatase, which indicates acidification of the resorption lacunae, was significantly higher for the coated implants, suggesting gradual resorption of the CaP coating. This study reveals detailed genetic responses to nanostructured CaP-coated implants and provides evidence that the effect of nanotopography is significant during the osseointegration cascade.

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“…Furthermore, a well‐evaluated osteoblast precursor cell line (MC3T3‐E1) was used to study the potential of a BMP2 tethered hybrid scaffold to promote osteogenic differentiation. MC3T3‐E1 pre‐osteoblasts have been shown to express elevated osteoblastic markers such as ALP, osteocalcin, osteopontin and bone sialoprotein when cultured with BMP‐2 (Yamaguchi et al, ; Yonezawa et al ., ). The results in the current study clearly showed a significant increase in expression of osteopontin, bone sialoprotein and Runx‐2 by the MC3T3‐E1 pre‐osteoblasts cultured in hybrid scaffolds tethered with rhBMP2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Design, fabrication andin vitroevaluation of a novel polymer-hydrogel hybrid scaffold for bone tissue engineering

Igwe

Mikael

Nukavarapu

2012

J Tissue Eng Regen Med

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The development of a bone mechanically-compatible and osteoinductive scaffold is important for bone tissue engineering applications, particularly for the repair and regeneration of large area critically-sized bone defects. Although previous studies with weight-bearing scaffolds have shown promising results, there is a clear need to develop better osteoinductive strategies for effective scaffold-based bone regeneration. In this study, we designed and fabricated a novel polymer-hydrogel hybrid scaffold system in which a load-bearing polymer matrix and a peptide hydrogel allowed for the synergistic combination of mechanical strength and great potential for osteoinductivity in a single scaffold. The hybrid scaffold system promoted increased pre-osteoblastic cell proliferation. Further, we biotinylated human recombinant bone morphogenetic protein 2 (rhBMP2), and characterized the biotin addition and its effect on rhBMP2 biological activity. The biotinylated rhBMP2 was tethered to the hybrid scaffold using biotin-streptavidin complexation. Controlled release studies demonstrated increased rhBMP2 retention with the tethered rhBMP2 hybrid scaffold group. In vitro evaluation of the hybrid scaffold was performed with rat bone marrow stromal cells and mouse pre-osteoblast cell line MC3T3-E1 cells. Gene expression of alkaline phosphatase (ALP), collagen I (Col I), osteopontin (OPN), bone sialoprotein (BSP), Runx-2 and osteocalcin (OC) increased in MC3T3-E1 cells seeded on the rhBMP2 tethered hybrid scaffolds over the untethered counterparts, demonstrating osteoinductive potential of the hybrid graft. These findings suggest the possibility of developing a novel polymer-hydrogel hybrid system that is weight bearing and osteoinductive for effective bone tissue engineering.

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Harmine promotes osteoblast differentiation through bone morphogenetic protein signaling

Cited by 68 publications

References 20 publications

Adherence to gastroprotection during cyclooxygenase 2 inhibitor treatment and the risk of upper gastrointestinal tract events: A population‐based study

Adherence to gastroprotection during cyclooxygenase 2 inhibitor treatment and the risk of upper gastrointestinal tract events: A population‐based study

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

Design, fabrication andin vitroevaluation of a novel polymer-hydrogel hybrid scaffold for bone tissue engineering

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