Haptoglobin Typing, is It Clinically Necessary for a Reliable Determination of Haptoglobin with the Single Radial Immunodiffusion Technique?

Rijn, H. J. M. Van; Kruit, Wim; Schrijver, J.

doi:10.1515/cclm.1984.22.1.109

Cited by 4 publications

(3 citation statements)

References 4 publications

(4 reference statements)

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“…Based on results obtained earlier in our laboratory (14,19,20) and the results obtained in this study, we advocate that phenotyping be performed only when indispensable for clinical purposes, i.e., when the uncorrected Hp value obtained from the RA-1000 is within the range 0.38-0.98 g/L. In this range the decision as regards lowered or normal depends on the phenotype.…”

Section: Discussionsupporting

confidence: 52%

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Rijn¹,

Wilt²,

Stroes³

et al. 1987

Clinical Biochemistry

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Comparison of the turNdirnetric immunossay of haptoglobin with a reference method (the RID technique with appropriate correction for phenetype) clearly showed the turbidimetric assay to be phenotypedependent. Correction f~ctors for the three main phenotypes were calculated and reference values determined.The observed overall reference range of haptoglobin, irrespective of the phenotype, was 0.56-3.76 g/L. After correction for phenotype, reference values for the three types were: Hp 1-1 : 0.77-2.49; Hp 2-1:0.86-4.76; and Hp 2-2:0.48-3.15 g Hp/L, From our reference values for the several phenotypes of haptoglobin it may be argued that phenotyping is desirable only when the uncorrected haptoglobin value, determined by turbidimetry, is within the range 0.38-0.98 g/L. Limited to hemolytic diseases, phenotype determination can be omitted beyond this range.

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Section: Discussionsupporting

confidence: 52%

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Rijn¹,

Wilt²,

Stroes³

et al. 1987

Clinical Biochemistry

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“…Relative molecular mass differs between haptoglobin types (Hp 1-1: M T = 80000, Hp 2-1: M T = 120000, Hp 2-2: M T = 160000). This leads to different diffusion rates between the haptoglobin types which results in an underestimation of Hp 2-2 concentration with the radial immunodiffusion technique (2,4,19). This might explain the lower Hp 1-1 and higher Hp 2-2 concentrations determined by nephelometry in this study compared to studies using radial immunodiftusion (6,7).…”

Section: Discussionmentioning

confidence: 80%

Evolution of Haptoglobin Concentration in Serum during the Early Phase of Acute Myocardial Infarction

Bernard¹,

Langlois²,

Delanghe³

et al. 1997

Clinical Chemistry and Laboratory Medicine

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is a haemoglobin-binding acute phase protein with three genetic types: Hp 1-1, Hp 2-1, Hp 2-2. We investigated 45 patients during the first 48 hours of acute myocardial infarction, and studied determinant factors and clinical correlates. Upon hospital admission, serum haptoglobin concentration was increased (1.95 ± 0.94 g/1, mean ± SD, P < 0.001) versus the reference population (0.97 ± 0.46 g/l, n = 107), independent of haptoglobin type: 1.84 ± 0.64 g/1 (Hp 1-1, n = 11) (P < 0.01), 1.98 ± 0.79 g/l (Hp 2-1, n = 25) (P < 0.001), 1.98 ± 1.58 g/l (Hp 2-2, n = 9) (P < 0.001). Moreover, during the first hours of hospitalization, a temporal lowering of haptoglobin was observed suggesting acute haemolysis, independent of the haptoglobin type. Minimal serum haptoglobin was reached 9.6 ± 5.8 hours after admission. The amplitude of the haptoglobin decrease correlated with initial serum haptoglobin (r = 0.78) and was more pronounced (P < 0.05) in men (0.53 ± 0.57 g/1) than in women (0.18 ± 0.17 g/1). Decrease of serum haptoglobin did not correlate with infarct size (based on creatine kinase-MB release). Out of the other acute phase proteins measured upon admission, only C-reactive protein was significantly increased (P < 0.05). During the next 36 hours, haptoglobin increased as a result of the acute phase response to myocardial injury. Our findings suggest that acute myocardial infarction is also preceded by an acute phase response, characterized by an initial high haptoglobin and followed by a temporal haptoglobin decrease due to haemolysis.

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“…7 Initially, the detection of haptoglobin was mainly based on the haptoglobin-haemoglobin binding capacity 28,29 or be measured immunochemically by radial immunodiffusion, immunonephelometric or immunoturbidimetric assays. 30,31 However, the results of these immunochemical methods are often less sensitive and influenced by haptoglobin genotypes. 32 The quantitative enzyme-linked immunosorbent assay (ELISA) method is proven to be highly sensitive and repeatable, and substantially less time consuming; so that it has been widely used in determining serum haptoglobin concentrations.…”

mentioning

confidence: 99%

Development of a sensitive and reliable ELISA kit of urinary haptoglobin to predict progress of diabetic kidney disease

Zheng

Yang

Chen

et al. 2021

Diabetes Metabolism Res

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Aims Urinary haptoglobin (UHp) is a potential biomarker for predicting progress of diabetic kidney disease (DKD) to remedy the defects of currently used urinary albumin. The clinical application of UHp is however limited, owing to the extremely low level in urine. This study aims to establish an enzyme‐linked immunosorbent assay (ELISA) kit specifically for detecting UHp in urine samples of patients with diabetes and DKD. Materials and Methods Supersensitive human haptoglobin antibodies were generated for ELISA kit development, and the sensitivity, specificity and reproducibility of the kit was evaluated. This kit was used to detect UHp in 246 healthy individuals and 83 patients with type 2 diabetes (T2D). The interference of blood haptoglobin genotypes on UHp measurement was analysed. Results The UHp ELISA kit had a standard curve ranging from 5 to 200 ng/ml. The low detection limit was 0.11 ng/ml. The coefficients of variation of intra‐ and interassay were 5.5% and 8.3%, respectively. The kit showed high accuracy with 100.9% mean recovery rate, and linearity R2 = 0.999. The reference range of UHp was 0–42.3 ng/g creatinine (0–Q95) in the healthy individuals. UHp level was significantly higher in T2D patients with microalbuminuria and macroalbuminuria than that in T2D without microalbuminuria (p < 0.01). The UHp concentration measured by this kit was not affected by haptoglobin genotypes. Conclusions We have generated an ELISA kit to accurately detect UHp levels, which is potentially a reliable biomarker of DKD.

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Haptoglobin Typing, is It Clinically Necessary for a Reliable Determination of Haptoglobin with the Single Radial Immunodiffusion Technique?

Cited by 4 publications

References 4 publications

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Is the turbidimetric immunoassay of haptoglobin phenotype-dependent?

Evolution of Haptoglobin Concentration in Serum during the Early Phase of Acute Myocardial Infarction

Development of a sensitive and reliable ELISA kit of urinary haptoglobin to predict progress of diabetic kidney disease

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