Haptoglobin reduces renal oxidative DNA and tissue damage during phenylhydrazine-induced hemolysis

Lim, Y.C.; Jenner, Andrew; Ali, Azhar; Wang, Yanping; Hsu, Stephen I‐Hong; Chong, Siew Meng; Baumman, Heinz; Halliwell, Barry; Lim, Sai‐Kiang

doi:10.1046/j.1523-1755.2000.00261.x

Cited by 97 publications

(88 citation statements)

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“…Haptoglobin appears to play an important physiological role as an antioxidant, particularly during haemolysis, i.e. the breakdown of red blood cells (Lim et al, 2000). Exercise-induced haemolysis is well documented in humans (Sentürk et al, 2005), and has been suggested to occur in birds (Landys-Ciannelli et al, 2002;Piersma et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Constitutive immune function in European starlings,Sturnus vulgaris, is decreased immediately after an endurance flight in a wind tunnel

Nebel

Bauchinger

Buehler

et al. 2012

Journal of Experimental Biology

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SUMMARYLife-history theory predicts that animals face a trade-off in energy allocation between performing strenuous exercise, such as migratory flight, and mounting an immune response. We experimentally tested this prediction by studying immune function in European starlings, Sturnus vulgaris, flown in a wind tunnel. Specifically, we predicted that constitutive immune function decreases in response to training and, additionally, in response to immediate exercise. We compared constitutive immune function among three groups: (1) ʻuntrainedʼ birds that were kept in cages and were not flown; (2) ʻtrainedʼ birds that received flight training over a 15day period and performed a 1-4h continuous flight, after which they rested for 48h before being sampled; and (3) ʻpost-flightʼ birds that differed from the ʻtrainedʼ group only in being sampled immediately after the final flight. A bird in our trained group represents an individual during migration that has been resting between migratory flights for at least 2days. A bird in our post-flight group represents an individual that has just completed a migratory flight and has not yet had time to recover. Three of our four indicators (haptoglobin, agglutination and lysis) showed the predicted decrease in immune function in the post-flight group, and two indicators (haptoglobin, agglutination) showed the predicted decreasing trend from the untrained to trained to post-flight group. Haptoglobin levels were negatively correlated with flight duration. No effect of training or flight was detected on leukocyte profiles. Our results suggest that in European starlings, constitutive immune function is decreased more as a result of immediate exercise than of exercise training. Because of the recent emergence of avian-borne diseases, understanding the trade-offs and challenges faced by long-distance migrants has gained a new level of relevance and urgency.

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Section: Discussionmentioning

confidence: 99%

Constitutive immune function in European starlings,Sturnus vulgaris, is decreased immediately after an endurance flight in a wind tunnel

Nebel

Bauchinger

Buehler

et al. 2012

Journal of Experimental Biology

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“…The covalent Hb/haptoglobin complex has long been considered the major pathway for directing CF-Hb clearance (1,5,41). Although studies (3,8,33) have demonstrated that haptoglobin limits Hb-induced hypertension and renal damage, a recent study (3) showed that haptoglobin did not decrease the half-life (t 1/2 ) of CF-Hb in the circulation or inhibit the ability of CF-Hb to react with NO. Additionally, CF-Hb clearance in haptoglobin-null mice was not altered compared with wildtype mice (32).…”

mentioning

confidence: 99%

A novel hemoglobin-binding peptide reduces cell-free hemoglobin in murine hemolytic anemia

Hanson

Flewelen

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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“…The formation of a complex between Hp and Hb also prevents Hb from passing through the glomeruli. Thus, mice lacking Hp display renal failure (4). Whether lower vertebrates also use Hp as a protective measure against the harmful effects of free Hb is not known.…”

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confidence: 99%

Haptoglobin, a hemoglobin-binding plasma protein, is present in bony fish and mammals but not in frog and chicken

Zernicka-Goetz

Fries

2006

Proc. Natl. Acad. Sci. U.S.A.

125

102

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Hemoglobin (Hb) released from erythrocytes may cause oxidation of lipids and proteins. Haptoglobin (Hp), which occurs in the plasma of all mammals, binds free Hb and inhibits its oxidative activity. It is not known whether this protective protein also exists in lower vertebrates. By analyzing available genomic sequences, we have found that bony fish, but not more primitive animals, have a gene coding for a protein homologous to mammalian Hp. Furthermore, we show that this protein is present in the plasma of Japanese pufferfish (Takifugu rubripes) and that it binds Hb. These results, together with a phylogenetic analysis, suggest that Hp evolved from a complement-associated protein (mannose-binding lectin-associated serine proteinase, MASP), with the emergence of fish. Surprisingly, we found that both chicken (Gallus gallus) and the Western clawed frog (Xenopus tropicalis) lack the Hp gene. In chicken plasma, however, we identified a different type of Hbbinding protein, PIT54, which has been reported to be a potent antioxidant. PIT54 is a soluble member of the family of scavenger receptor cysteine-rich proteins, and we found that its gene exists only in birds. We also show that the plasma of ostrich (Strutio camelus), a primitive bird, contains both PIT54 and Hp. Collectively, our data suggest that PIT54 has successively taken over the function of Hp during the evolution of the avian lineage and has completely replaced the latter protein in chicken.evolution ͉ PIT54 ͉ complement proteinase

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Haptoglobin reduces renal oxidative DNA and tissue damage during phenylhydrazine-induced hemolysis

Abstract: Haptoglobin appears to play an important physiological role as an antioxidant, particularly during hemolysis.

Cited by 97 publications

References 50 publications

Constitutive immune function in European starlings,Sturnus vulgaris, is decreased immediately after an endurance flight in a wind tunnel

Constitutive immune function in European starlings,Sturnus vulgaris, is decreased immediately after an endurance flight in a wind tunnel

A novel hemoglobin-binding peptide reduces cell-free hemoglobin in murine hemolytic anemia

Haptoglobin, a hemoglobin-binding plasma protein, is present in bony fish and mammals but not in frog and chicken

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