Hapten Synthesis for the Development of a Competitive Inhibition Enzyme-Immunoassay for Thiram

Gueguen, Françoise; Boisdé, Frédéric; Queffelec, Anne-Laurence; Haelters, Jean‐Pierre; Thouvenot, Daniel; Corbel, Bernard; Nodet, Patrice

doi:10.1021/jf000378g

Cited by 26 publications

(15 citation statements)

References 26 publications

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“…On the other hand, experience in antibody production against small aliphatic structures in hapten immunochemistry is limited. A sporadic attempt to produce antibodies against an underivatised small hapten structure was accomplished by Gueguen et al using immunogens with a di-thiocarbamate group attached to a carrier protein [22]. Surprisingly, antibody formation against small thiram pesticide was evoked, although antibodies exhibited very low sensitivity when incorporated into an indirect ELISA system.…”

Section: Analyte and Hapten Designmentioning

confidence: 99%

ELISA for semicarbazide and its application for screening in food contamination

Vass

Diblíková

Cernoch

et al. 2008

Analytica Chimica Acta

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Section: Analyte and Hapten Designmentioning

confidence: 99%

ELISA for semicarbazide and its application for screening in food contamination

Vass

Diblíková

Cernoch

et al. 2008

Analytica Chimica Acta

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“…Black polystyrene high-binding plates were from Costar (Cambridge, Massachusetts). The hapten-protein conjugate (OVA-2C) and the polyclonal antibodies against thiram were prepared as previously described (Gueguen 2000). 5…”

Section: Reagentsmentioning

confidence: 99%

“…Various analytical methods to determine both qualitatively and quantitatively thiram have been reported, based on spectrophotometric (Tunceli 2001) chromatographic (Gustafsson on 1981;Brandsteterova 1986) and immuno-enzymatic (Gueguen 2000;Queffelec 2001;Aulakh 2005) techniques.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Thiram in Honeybees: Development of a Chemiluminescent ELISA

Girotti¹,

Maiolini²,

Ghini³

et al. 2008

Analytical Letters

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International audienceA Chemiluminescence Enzyme-Linked Immuno-Sorbent Assay (CL-ELISA) for determination and quantification of the fungicide thiram in honeybees was developed in an indirect competitive format. The assay was optimized by determining: the optimal coating conjugate concentration and anti-thiram antiserum dilution, the effect of the incubation time on the competitive step, the tolerance to organic solvents. The IC50 and the limit of detection (LOD) values were 60 ng mL-1 and 9 ng mL -1, respectively, similar to those of colorimetric ELISA with a calibration range of 9 – 15,000 ng mL-1. Cross reactivity of some related compounds such as some dithiocarbamates, a thiocarbamate, the ethylenethiourea and the tetramethylthiourea were tested. The assay was then applied to honeybees sample extracts obtained by using the liquid-liquid extraction or the graphitized carbon-based solid phase extraction. The calibration curves in honeybee extracts from liquid-liquid procedure gave an IC50 of 141 ng mL-1 and a LOD of 17 ng mL-1. In case of extracts obtained by SPE these values were 139 ng mL-1 and 15 ng mL-1, respectively. The average recovery value from honeybee extracts spiked with 75 ng mL-1 of thiram was 72% for SPE, higher than for liquid-liquid extraction (60%). On the opposite, when the honeybees were directly spiked with 2 and 10 ppm the average recovery was higher for liquid-liquid extraction (54%), than for SPE (31%). Finally, the assay was applied to honeybee samples collected during monitoring activities in Italy and Russia

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“…Many methods like spectrophotometry, 1,[3][4][5][6][7][8] flame atomic absorption spectrometry 9,10 FT-IR, 11 gas chromatography, 12,13 HPLC, [14][15][16] polarography, 17,18 biosensors, 19 and enzyme linked immuno sorbent assay 20 are available for Zineb determination and other dithiocarbamates. Most of the applied methods use different techniques like spectrophotometry and gas chromatography 3 based on analysis of CS 2 , H 2 S or amines which are evolved by the decomposition of EBDCs.…”

Section: Introductionmentioning

confidence: 99%

Development of a derivative spectrophotometric method for the determination of fungicide zinc ethylenebisdithiocarbamate using sodium molybdate

Kaur¹,

Kaur²,

Malik³

et al. 2009

J. Braz. Chem. Soc.

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Neste trabalho foi desenvolvido um procedimento espectrofotométrico derivativo para a determinação de etilenobisditiocarbamato de zinco(II), Zineb, com base na formação do complexo azul de molibdato de sódio em meio ácido. A lei de Beer é obedecida até 40 µg mL -1 de Zineb no comprimento de onda de 956 nm. O limite de detecção foi de 0,006 µg mL -1 de Zineb para a razão S/N = 3 considerando diversos parâmetros como o efeito da concentração de ácido. A interferência de diversos íons na determinação de Zineb foi avaliada. A maioria dos metais alcalinos e sais metálicos não causou interferências. O procedimento tem boa sensibilidade e foi empregado para a determinação de Zineb em amostras comerciais de alimentos contendo Dithane Z 78 e Hexathane 75% W.P.. Os resultados foram comparados com procedimentos previamente propostos. Zineb foi determinado com sucesso sem a ocorrência de interferências na presença de outros ditiocarbamatos, tais como Ziram, Thiram, Ferbam etc.A derivative spectrophotometric procedure was developed for the determination of zinc(II) ethylenebisdithiocarbamate, Zineb, after formation of its blue colored complex with sodium molybdate in acidic medium. The Beer's law is obeyed up to 40 µg mL -1 of Zineb at 956 nm. The detection limit was 0.006 µg mL -1 for Zineb when S/N ratio is 3 taking into account various parameters, such as the effect of acid concentration. The interference of a large number of ions on the determination of Zineb was evaluated. Most of the alkaline metals and metal salts did not interfere. The procedure presented proper sensitivity and it was applied for determining Zineb in food stuffs and commercial samples of Dithane Z 78 and Hexathane 75% W.P.. Results were compared with earlier reported methods. Zineb was successfully determined without any interferences in the presence of other dithiocarbamates like ziram, thiram, ferbam etc.

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Hapten Synthesis for the Development of a Competitive Inhibition Enzyme-Immunoassay for Thiram

Cited by 26 publications

References 26 publications

ELISA for semicarbazide and its application for screening in food contamination

ELISA for semicarbazide and its application for screening in food contamination

Quantification of Thiram in Honeybees: Development of a Chemiluminescent ELISA

Development of a derivative spectrophotometric method for the determination of fungicide zinc ethylenebisdithiocarbamate using sodium molybdate

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