ELISA for semicarbazide and its application for screening in food contamination

Vass, M.; Diblíková, I; Cernoch, Ivo; Fránek, Milan

doi:10.1016/j.aca.2007.11.052

Cited by 49 publications

(55 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For nitrofuran metabolites, haptens were always designed by derivatising the metabolites with 3-CBA or 4-CBA [6,7,[9][10][11][12][13][14][15]. Although this strategy was able to generate antibodies with high specificity and sensitivity, it's believed that there are some other strategies to further improve the antibody characteristics.…”

Section: Design and Synthesis Of Haptensmentioning

confidence: 99%

“…It is well known that nitrofuran drugs are metabolized rapidly in vivo, and as a consequence the significant cross-reactivity with furaltadone is unlikely to affect the determined results in practice. The negligible assay response to o-NBA (<0.02%) eliminates the need for separation of this reagent from the sample homogenate after derivatisation when employing published extraction procedures [12]. …”

Section: Antibody Specificitymentioning

confidence: 99%

“…The first enzyme-linked immunosorbent assay (ELISA) capable of detecting the furazolidone metabolite (AOZ) was developed by Cooper et al [6] under the support of a multi-national EU research project "FoodBRAND" [2]. Since then, immunoassays for the determination of AOZ [7][8][9], SEM [10][11][12], AHD [13,14] and AMOZ [15] were reported in succession.…”

Section: Introductionmentioning

confidence: 99%

“…NPAOZ, NASEM, NPAHD and NPAMOZ) [6][7][8][9][10][11][12][13][14][15]. In most studies, nitrofuran metabolites were derivatised with 3-carboxybenzaldehyle (3-CBA) or 4-carboxybenzaldehyle (4-CBA) to form immunizing haptens [6,7,[9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Shen

Sun

et al. 2013

Talanta

View full text Add to dashboard Cite

Publisher rights This is the author's version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, VOL 103, 01/2012 General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Cite this article as: Zhen-Lin Xu, Yu-Dong Shen, Yuan-Ming Sun, Katrina Campbell, Yuan-Xin Tian, Shi-Wei Zhang, Hong-Tao Lei and Yue-Ming Jiang, Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), Talanta, http://dx.doi.org/10. 1016/j.talanta.2012.10.059 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractA heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of the furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) was developed. AMOZ was derivatised with 2-(4-formylphenoxy)acetic acid or 2-(3-formylphenoxy)acetic acid to obtain two novel immunizing haptens. The ability of these haptens in producing specific polyclonal antibodies against the nitrophenyl derivative of AMOZ (NPAMOZ) was compared with that of traditional immunizing haptens (derivatised AMOZ with 3-carboxybenzaldehyle or 4-carboxybenzaldehyle). The results indicated that the novel immunizing haptens were able to produce antibodies with almost a two-fold improvement in sensitivity of the ciELISA for NPAMOZ in comparison with the existing antibody based ELISAs. The differences in sensitivity were explained by the molecular modeling of the lowest energy conformations of NPAMOZ and the haptens. Another novel hapten, derivatised AMOZ with 2-oxoacetic acid, was synthesized and used as a heterologous coating hapten. The results showed that this strategy of using only a partial structure of the target molecule as the coating hapten was able to obtain a two to three-fold improvement in sensitivity. This study provided a modern approach for the development of an immunoassay with improved sensitivity for the metabolites of nitrofuran antibiotics.

show abstract

Section: Design and Synthesis Of Haptensmentioning

confidence: 99%

Section: Antibody Specificitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Shen

Sun

et al. 2013

Talanta

View full text Add to dashboard Cite

show abstract

“…Liu et al produced polyclonal antibodies against CPAHD that were specific to nitrofurantoin and used for in drinking water [20]. Similar approaches were undertaken for the development of the ELISA formats based on monoclonal and polyclonal antibodies against CPSEM as a metabolic target of nitrofurazone by Cooper et al [13], Gao et al [15] and Vass et al [24], which were considered to be effective screening assays for porcine muscle, chicken tissue and egg samples.…”

mentioning

confidence: 99%

Development of a Competitive ELISA for the Detection of a Furaltadone Marker Residue, 3-Amino-5-Morpholinomethyl-2-Oxazolidinone (AMOZ), in Cultured Fish Samples

Sheu

Tai²,

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. This report describes an enzyme-linked immunosorbent assay (ELISA) for tissue-bound metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) and the application to residue analysis in cultured fish samples. The residue is monitored as a marker for the drug furaltadone. The assay enables the detection of protein bound AMOZ in the form of a 2-nitrophenyl derivative (2-NP-AMOZ) in sample supernatant or extract after acid hydrolysis and derivatization with o-nitrobenzaldehyde. Polyclonal rabbit antibodies were produced with a new immunogen hapten, 2-NP-HXA-AMOZ. The new ELISA had adequate analytical sensitivity (IC 50 value 0.325 µg kg −1 ; limit of detection 0.1 µg kg −1 ) to determine a trace of AMOZ residue and had a high selectivity. Recoveries of AMOZ fortified at the levels of 0.1, 0.5 and 1.0 µg kg −1 ranged from 89.8 to 112.5% with coefficients of variation of 12.4−16.2% over the range of AMOZ concentrations studied. The results obtained with the ELISA correlated well with those obtained by commercial test kits for 150 tested samples (r=0.984). The results suggest that the developed ELISA is a highly specific, accurate, and sensitive method suitable for high throughput screening for AMOZ residues.

show abstract

Voltammetric Behavior of Semicarbazide at Graphene Modified Electrode and Application to Detection

Zhang

et al. 2014

J Chinese Chemical Soc

View full text Add to dashboard Cite

Herein voltammetric behaviors of semicarbazide (SEM) are investigated by employing a graphene modified working electrode, which displays attractive electroanalytical properties. In the acetate buffer solution of pH 4.00, there is a well-defined oxidative peak of SEM, attributing to the irreversible and adsorption-controlled electrode reaction with 2 electrons participation. The values of apparent heterogeneous electron transfer rate constant k¢ s with the redox are 0.0061 s -1 and 0.0009 s -1 , respectively, for two scan rate section. After the experimental parameters, which influence the voltammetric responses of SEM, including supporting electrolyte, pH, accumulation time and accumulation potential, etc., are optimized, it is found that the anodic peak current of SEM is proportional to its concentration in the range of 4 -40 mmol/L with a detection limit of 1 mmol/L (S/N = 3). Then, an electrochemical method for detecting SEM quantitatively is developed successfully. The concentrations of SEM in fortified tap water samples are tested with satisfactory recovery, indicating that the novel method is strongly promising in the environmental monitoring application.

show abstract

ELISA for semicarbazide and its application for screening in food contamination

Cited by 49 publications

References 21 publications

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Development of a Competitive ELISA for the Detection of a Furaltadone Marker Residue, 3-Amino-5-Morpholinomethyl-2-Oxazolidinone (AMOZ), in Cultured Fish Samples

Voltammetric Behavior of Semicarbazide at Graphene Modified Electrode and Application to Detection

Contact Info

Product

Resources

About