Hapten-Grafted Programmed Probe as a Corecognition Element for a Competitive Immunosensor to Detect Acetamiprid Residue in Agricultural Products

Xu, Xiahong; Guo, Yi; Wang, Liu; He, Kaiyu; Guo, Yirong; Wang, Xinquan; Gunasekaran, Sundaram

doi:10.1021/acs.jafc.8b02487

Cited by 21 publications

(9 citation statements)

References 42 publications

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“…Therefore, chromatography‐based methods are unsuitable for in situ , nondestructive and rapid detection. In addition, immunoassay can be used as an alternative method for acetamiprid residue analysis, 9,10 but the method often lacks sensitivity and reproducibility and is expensive. Similarly, the immunology‐based approach is also not suitable for in situ and nondestructive detection.…”

Section: Introductionmentioning

confidence: 99%

In situ and rapid determination of acetamiprid residue on cabbage leaf using surface‐enhanced Raman scattering

Guo

Lü

2020

J Sci Food Agric

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BACKGROUND Pesticide residues in agricultural products and foods pose a serious threat to human health, and therefore a simple, rapid and direct method is urgently needed for pesticide residue detection. In addition to realizing the detection of acetamiprid in cabbage extract solution, the main target of this study was to establish an in situ surface‐enhanced Raman scattering (SERS) method, which could directly detect acetamiprid residue on cabbage leaf without the need for extraction. Acetamiprid was first used to contaminate the surface of fresh cabbage leaf, and then bimetallic silver‐coated gold nanoparticles (Au@AgNPs) were added on the contaminated spots and dried for SERS measurement. RESULTS Results suggested that acetamiprid can be detected in cabbage extract and on cabbage leaf surface in situ using the SERS method based on the Au@AgNPs substrate. The limit of detection was 0.08 μg mL−1 in cabbage extract and 0.14 mg kg−1 on cabbage leaf, the recovery ranged from 80.5% to 105.5% and the relative standard deviation was in the range 4.37–10.63%. CONCLUSIONS The proposed SERS method provides an in situ, nondestructive and rapid way to detect acetamiprid residue on the surface of fruits and vegetables, which could serve as an auxiliary approach for early screening of contaminated produce in field or on site in the future. © 2020 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

In situ and rapid determination of acetamiprid residue on cabbage leaf using surface‐enhanced Raman scattering

Guo

Lü

2020

J Sci Food Agric

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“…None of the potential interference pesticides showed considerable current change within a neonicotinoid reduction potential window of −1.18 to −1.24 V. The chosen herbicide species atrazine, glyphosate, dicamba, and 2,4-dichlorophenoxyacetic acid have become some of the most prevalent herbicides applied in the Midwest and pose threats of aerosolization and watershed contamination. , Additionally, the insecticides parathion and paraoxon were investigated as both have shown electroactive behavior between 0 and −0.4 V vs Ag/AgCl, − a potential window that is inconsequential to the neonicotinoid sensing regime. Neonicotinoid studies to date have not reported a comprehensive pesticide interferent species analysis, as shown in Figure . ,, …”

Section: Resultsmentioning

confidence: 99%

“…Neonicotinoid studies to date have not reported a comprehensive pesticide interferent species analysis, as shown in Figure 4. 66,69,76 Finally, recovery tests were performed in water from the South Skunk River in the state of Iowa. The water was filtered through a 0.45 μm filter, brought to 0.5 M NaCl, and spiked with 40 μM of a neonicotinoid.…”

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confidence: 99%

Electrochemical Sensing of Neonicotinoids Using Laser-Induced Graphene

et al. 2021

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Neonicotinoids are the fastest-growing insecticide accounting for over 25% of the global pesticide market and are capable of controlling a range of pests that damage croplands, home yards/gardens, and golf course greens. However, widespread use has led to nontarget organism decline in pollinators, insects, and birds, while chronic, sublethal effects on humans are still largely unknown. Therefore, there is a need to understand how prevalent neonicotinoids are in the environment as there are currently no commercially available field-deployable sensors capable of measuring neonicotinoid concentrations in surface waters. Herein, we report the first example of a laser-induced graphene (LIG) platform that utilizes electrochemical sensing for neonicotinoid detection. These graphene-based sensors are created through a scalable direct-write laser fabrication process that converts polyimide into LIG, which eliminates the need for chemical synthesis of graphene, ink formulation, masks, stencils, pattern rolls, and postprint annealing commonly associated with other printed graphene sensors. The LIG electrodes were capable of monitoring four major neonicotinoids (CLO, IMD, TMX, and DNT) with low detection limits (CLO, 823 nM; IMD, 384 nM; TMX, 338 nM; and DNT, 682 nM) and a rapid response time (∼10 s) using square-wave voltammetry without chemical/biological functionalization. Interference testing exhibited negligible responses from widely used pesticides including the broad-leaf insecticides parathion, paraoxon, and fipronil, as well as systemic herbicides glyphosate (roundup), atrazine, dicamba, and 2,4-dichlorophenoxyacetic acid. These scalable, graphene-based sensors have the potential for wide-scale mapping of neonicotinoids in watersheds and potential use in numerous electrochemical sensor devices.

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“…Some examples of electrochemical immunosensors are reported for the detection of neonicotinoids [132,133], especially with aptamers [134,135] but in our knowledge, only one work has published on immunosensor for detection of acetamiprid [136]. This work is based on hapten-grafted programmed probe (HGPP) that competes with acetamiprid for the sites of an antibody-modified gold electrode (Figure 7).…”

Section: Immunosensors For Pesticide Determinationmentioning

confidence: 99%

“…( A ) Preparation of HGPP. ( B ) Competitive immunosensor assay for the detection of acetamiprid (reprinted with permission from [136]).…”

Section: Figurementioning

confidence: 99%

Trends and Perspectives in Immunosensors for Determination of Currently-Used Pesticides: The Case of Glyphosate, Organophosphates, and Neonicotinoids

et al. 2019

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Pesticides, due to their intensive use and their peculiar chemical features, can persist in the environment and enter the trophic chain, thus representing an environmental risk for the ecosystems and human health. Although there are several robust and reliable standard analytical techniques for their monitoring, the high frequency of contamination caused by pesticides requires methods for massive monitoring campaigns that are capable of rapidly detecting these compounds in many samples of different origin. Immunosensors represent a potential tool for simple, rapid, and sensitive monitoring of pesticides. Antibodies coupled to electrochemical or optical transducers have resulted in effective detection devices. In this review, the new trends in immunosensor development and the application of immunosensors for the detection of pesticides of environmental concern—such as glyphosate, organophosphates, and neonicotinoids—are described.

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Hapten-Grafted Programmed Probe as a Corecognition Element for a Competitive Immunosensor to Detect Acetamiprid Residue in Agricultural Products

Cited by 21 publications

References 42 publications

In situ and rapid determination of acetamiprid residue on cabbage leaf using surface‐enhanced Raman scattering

In situ and rapid determination of acetamiprid residue on cabbage leaf using surface‐enhanced Raman scattering

Electrochemical Sensing of Neonicotinoids Using Laser-Induced Graphene

Trends and Perspectives in Immunosensors for Determination of Currently-Used Pesticides: The Case of Glyphosate, Organophosphates, and Neonicotinoids

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