Haplotype diversity of the myostatin gene among beef cattlebreeds

Dunner, S.; Miranda, Molly; Amigues, Yves; Cañón, Javier; Georges, Michel; Hanset, R.; Williams, John L.; Ménissier, François

doi:10.1186/1297-9686-35-1-103

Cited by 111 publications

(51 citation statements)

References 29 publications

(39 reference statements)

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“…The high degree of polymorphism of the DNA sequence of the myostatin gene, which has been demonstrated in previous studies [1,6,12], was further displayed here in three elite commercial broiler chicken lines. Of the identified 13 SNPs, 11 were in exons and 2 were in introns.…”

Section: Discussionsupporting

confidence: 73%

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Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens

Brown

Nones³

et al. 2007

Genet. Sel. Evol.

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-Myostatin is a negative regulator of skeletal muscle growth. We evaluated effects of myostatin polymorphisms in three elite commercial broiler chicken lines on mortality, growth, feed conversion efficiency, ultrasound breast depth, breast percentage, eviscerated carcass weight, leg defects, blood oxygen level, and hen antibody titer to infectious bursal disease virus vaccine. Progeny mean data adjusted for fixed and mate effects and DNA from 100 sires per line were used. Single nucleotide polymorphisms (SNPs) of the myostatin gene segregating in these lines were identified by designing specific primers, amplifying individual DNA in each line by polymerase chain reaction, cloning, sequencing and aligning the corresponding products. Individual sires were genotyped for five identified SNPs which contributed to eight haplotypes. Frequencies of SNP alleles and haplotypes differed between lines. Using the allele substitution effect model, the myostatin SNPs were found to have significant (P < 0.031) associations with growth, mortality, blood oxygen and hen antibody titer to infectious bursal disease virus vaccine, although the associations were not often consistent across lines. These results suggest that the myostatin gene has pleiotropic effects on broiler performance. myostatin / SNP / growth / mortality / broiler chicken

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Section: Discussionsupporting

confidence: 73%

“…The myostatin gene is highly polymorphic. Nineteen single nucleotide polymorphisms (SNPs) and 20 haplotypes have been detected in 28 European bovine breeds, of which some were breed-specific [6]. Baron et al [1] identified seven SNPs and one deletion in exon 2 of the myostatin gene in broiler and/or layer chicken lines.…”

Section: Introductionmentioning

confidence: 99%

Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens

Brown

Nones³

et al. 2007

Genet. Sel. Evol.

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“…Different techniques, such as PCR restriction fragment length polymorphism (RFLP), single strand conformational polymorphism (SSCP), oligonucleotide ligation assay (OLA) and fluorescent primer-based PCR assay, are described in the literature for the identification of mutations in MSTN gene (Dunner et al, 2003). However, most of these techniques require several procedural steps involving handling operations, running time for sample processing and risks of laboratory errors.…”

Section: Resultsmentioning

confidence: 99%

Comparison of three PCR-based methods to detect a Piedmontese cattle point mutation in the Myostatin gene

Pozzi

Bongioni

Galli

2009

Animal

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Despite many recent advances in single nucleotide polymorphism (SNP) genotyping technologies, there is still the need for methodologies with a reasonable throughput. In this study, we compared three PCR-based methods for SNP detection: (1) a conventional PCR-based allele detection system with fluorescent genotyping technology, (2) a SNaPshot methodology by single nucleotide primer extension and, (3) a real-time PCR-based method by allele-specific minor groove-binder probes. These three methodologies were used to analyze 104 meat samples of a particular Italian cattle breed known for producing excellent quality meat and for a characteristic increased development of muscle mass, caused by a point mutation (C313Y) in the Myostatin gene. The analysis revealed 98 samples to be homozygous (mh/mh) and five to be heterozygous (mh/1) for the mutation whereas one sample resulted to be homozygous for the wild type (1/1). The results obtained with the three different assays were consistent. Overall, all three methodologies proved to be efficient for allelic discrimination studies; however, real-time PCR was faster and allowed to genotype up to 96 samples in a single step, minimizing the number of steps required for samples manipulation.

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“…In allele-specific genotyping, homozygous CC genotypes showed a single-melting temperature peak, and heterozygous AC genotypes were identified by the presence of two peaks. Previous studies have shown that the mutant A allele of the MSTN SNP has high frequency in Limousin animals (0.83) and that it was identified in Charolais, Blonde d'Aquitane and Angus cattle (Dunner et al, 2003;Sellick et al, 2007), which are all B. taurus. Vankan et al (2009) Genotype AA of the MYOD1 polymorphism NC_007313:g.1274A>G was identified by the presence of two restriction fragments of 319 and 155 bp.…”

Section: Resultsmentioning

confidence: 99%

“…MSTN regulates skeletal muscle mass, and the SNP AF_320998.1:g.433C>A (F94L) is a non-synonymous mutation identified in the coding region of the gene. Independent studies have indicated that F94L increases beef yield in B. taurus cattle carcasses without the disadvantages of double muscling effects (Alexander et al, 2007;Sellick et al, 2007;Esmailizadeh et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus

Curi

Krauskopf

Hadlich

et al. 2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effects of single-nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C>T), MSTN (AF_320998.1:g.433C>A), MYOD1 (NC_007313:g.1274A>G) and MYF5 (NC_007303:g.1911A>G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non-informative in Nelore animals. In crossbred animals, the IGF1 C allele was associated with greater REA. However, this relation was not significant after Bonferroni correction for multiple testing. The A allele of the MSTN polymorphism was absent in Nelore cattle and was only found in two crossbred animals. The polymorphisms of MYOD1 and MYF5 were little informative in Nelore animals with G allele frequency of 0.097 and A allele frequency of 0.031, respectively. These markers show no association with the analyzed traits in the total sample of evaluated animals.

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Haplotype diversity of the myostatin gene among beef cattlebreeds

Cited by 111 publications

References 29 publications

Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens

Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens

Comparison of three PCR-based methods to detect a Piedmontese cattle point mutation in the Myostatin gene

Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus

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