Haploid plant regeneration from anther cultures of three north american cultivars of strawberry (Fragaria x ananassa Duch.)

Owen, Henry; Miller, Alexander

doi:10.1007/bf00231585

Cited by 26 publications

(9 citation statements)

References 13 publications

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“…Leaf disks were selected as the model explants on the base on literature data. Strawberry plants have been regenerated not only from leaf explants (Jones et al, 1988;Liu and Sanford, 1988;Nehra et al, 1989Nehra et al, , 1990aJames et al, 1990b;Sorvari et al, 1993;Wawrzynczak et al, 1990;Passey et al, 2003) but also from runner tissues (Liu and Sanford, 1988), petioles (Rugini and Orlando, 1992;Passey et al, 2003), stem tissue (Graham et al, 1995), excised cotyledons of mature achenes (Miller and Chandler, 1990), protoplasts (Wallin et al, 1993), peduncle and stolon segments (Lis, 1993), anthers (Svensson and Johansson, 1994;Owen and Miller, 1996), mesophyll protoplasts (Nyman and Wallin, 1988), roots (Rugini and Orlando, 1992;Passey et al, 2003), stipules (Rugini and Orlando, 1992;Passey et al, 2003) and immature embryos (Wang et al, 1984). In comparable analysis the percentage of regenerated plants originated from leaf fragments was significantly higher than from other explants for most out of the analyzed cultivars (Passey et al, 2003).…”

Section: Optimization Of Leaf Explants Regenerationmentioning

confidence: 98%

Conditions of transformation and regeneration of `Induka' and `Elista' strawberry plants

Gruchała

Korbin

Žurawicz

2004

Plant Cell, Tissue and Organ Culture

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Efficiency of plants' transformation depends on many factors. The genotype, applied techniques and conditions of plant's modification and modified plant regeneration are the most important among them. In our studies regeneration and transformation conditions for two strawberry cultivars were determined and compared. Plants were transformed by Agrobacterium tumefaciens LBA 4404 strain containing plasmid pBIN19 with nptII and gus-reporter genes. Experiment was carried out on more than 1300 leaf explants from each cultivar. Generally, 'Induka' plants characterized with higher regeneration potential than 'Elista'. The highest number of regenerated shoots was obtained on MS medium with 0.4 mg l À1 IBA and 1.8 mg l À1 BA (3.5 and 1.8 shoots/explant for 'Induka' and 'Elista', respectively). After plant transformation number of regenerated, transgenic shoots was higher for 'Elista' (on the average: 8.3 shoots/100 explants). The number of transgenic 'Induka' shoots, obtained at the same conditions, was twice lower (~4.2). Simultaneously 'Induka' plants needed higher kanamycin concentration for transgenic explants selection than 'Elista' (25 mg l À1 ). Preliminary incubation of A. tumefaciens in LB or MS medium with acetosyringone and IAA resulted in increasing transgenic shoots number (per 100 explants: 'Induka' 4.5, 'Elista' 8.0-9.5 shoots). After using untreated bacteria for plants' transformation, number of transgenic plants varied (dependently on cultivar) from 3.8 to 7.0/100 explants. Applying LB or MS as basic medium as well as adding tobacco plant extract to these media did not significantly influence transformation efficiency.Abbreviations: AS -acetosyringone; BA -benzyladenine; CTAB -hexadecyl trimethylammonium bromide; gus -ß-glucoronidase; IAA -indole-3-acetic acid; IBA -indole-3-butyric acid; LB -Luria Broth base; MS -Murashige and Skoog (1962); NOS -nopaline synthase promoter; nptII -neomycin phosphotransferase

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Section: Optimization Of Leaf Explants Regenerationmentioning

confidence: 98%

Conditions of transformation and regeneration of `Induka' and `Elista' strawberry plants

Gruchała

Korbin

Žurawicz

2004

Plant Cell, Tissue and Organ Culture

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“…To date, adventitious shoot regeneration has been demonstrated from a variety of in vitro cultured explants of the cultivated strawberry (Fragaria × ananassa Duch.). These include leaf explants (Jones et al 1988;Lui and Sandford 1988;Nehra et al 1989;Sorvari et al 1993), petioles (Foucault and Letouze 1987;Rugini and Orlando 1992), stipules (Rugini and Orlando 1992), stem tissue (Graham et al 1995), runner tissue (Liu and Sandford 1988), the peduncular base of the flower bud (Foucault and Letouze 1987), mesophyll protoplasts (Nyman and Wallin 1988), anther cultures (Owen and Miller 1996), roots (Rugini and Orlando 1992) and immature embryos (Wang et al 1984).…”

Section: Introductionmentioning

confidence: 97%

Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria × ananassa Duch.) using a range of explant types

2003

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The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf discs, petioles, roots and stipules. Regeneration from cv. Elsanta proved to be difficult from all explant material, although unpollinated ovaries proved to be a promising explant source, with 12% of the explants regenerating shoots. In cv. Eros, regeneration occurred only from root tissue. A comparison of the genetic background suggests that there is a strong genetic component amongst the different cultivars determining their regeneration capacity. The development of these regeneration systems provides a means to use almost the whole stock plant for the efficient genetic transformation of commercial strawberry varieties.

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“…Adventitious buds and shoots regeneration has been previously reported from different explants such as: leaves, [8][9][10][11][12][13][14][15][16] petioles, 11,12,15,17,18 peduncles/peduncular base of the flower buds, 17,18 stems, 19 stipules, 11,20 stolon, 18 roots, 11,20 runners, 9 and anther cultures. 21 Furthermore, shoot regeneration was produced directly from field, 10 greenhouse-grown strawberry plants, 12,15,22 or from in vitro-grown shoots. 1,11 Transformation in strawberries has been previously documented using different constructs in various genotypes.…”

Section: Introductionmentioning

confidence: 99%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome, which makes the breeding of new cultivars difficult. simple and efficient methods for transformation and regeneration are required for cultivars improvement in strawberries. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, sweet charlie, and Florida via direct organogenesis using the in vitro juvenile leaves as explants. explants were collected after sub-culturing on a propagation medium composed of Ms supplemented with 0.5 mg/l Ba; 0.1 mg/l Ga3 and 0.1 mg/l IBa. To select the suitable organogenesis, the explants of the three cultivars were cultured on Ms medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °c ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival and 73% for sweet charlie and Florida). after 4 weeks the produced shoots were cultured on Ms medium with different concentrations of Ba and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l Ba and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and sweet charlie, respectively. On the other hand, medium media containing 1.5 mg/l Ba and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. elongated shoots were successfully rooted on Ms medium containing 1.5 mg/l Naa. Furthermore, transformation of the two cultivars, Festival and sweet charlie, has been established via Agrobacterium strain LBa44404 containing the plasmid pIsV2678 with gus-intron and bar genes. Three days post-cocultivation, GUs activity was screened using the histochemical assay. The results showed 16% and 18% of the tested plant materials had changed into a blue color for Festival and sweet charlie, respectively. Out of 120 explants only 13 shoots were developed on bialaphos medium for each cultivar, representing 10.8% bialaphos-resistant strawberry shoot. The presence of both the genes bar and uid a was detected by PcR and Northern, giving a transformation efficiency of 5%.

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Haploid plant regeneration from anther cultures of three north american cultivars of strawberry (Fragaria x ananassa Duch.)

Cited by 26 publications

References 13 publications

Conditions of transformation and regeneration of `Induka' and `Elista' strawberry plants

Conditions of transformation and regeneration of `Induka' and `Elista' strawberry plants

Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria × ananassa Duch.) using a range of explant types

Improved regeneration and transformation protocols for three strawberry cultivars

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