Haploid Fertility in Temperate and Tropical Maize Germplasm

Kleiber, Daniel; Prigge, Vanessa; Melchinger, Albrecht E.; Burkard, Florian; Vicente, Félix San; Palomino, Guadalupe; Gordillo, G. Andrés

doi:10.2135/cropsci2011.07.0395

Cited by 54 publications

(101 citation statements)

References 21 publications

(32 reference statements)

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“…Strong to moderate correlations were found between haploid and DH lines (Table 3). S4) were found in various genetic materials (Geiger and Schönleben, 2011;Kleiber et al, 2012). Preselection among untreated haploid plants directly sown in the field should therefore result in improved per se performance at the DH level.…”

Section: Discussionmentioning

confidence: 99%

Genetic Correlations among Haploids, Doubled Haploids, and Testcrosses in Maize

2013

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Doubled haploid (DH) lines produced via in vivo haploid induction have become an indispensable tool in maize (Zea mays L.) breeding and research. To determine the predictive value of the per se performance of haploid and DH lines in population and hybrid breeding, genetically balanced sets of haploid and DH lines along with testcrosses of the DH lines were evaluated in field trials across four locations over 2 yr in Germany. Suitable material sets were provided by three collaborating breeders. Each set comprised 54 to 58 DH lines developed from a proprietary elite dent single cross. These DH lines were crossed with one or two flint testers and subjected to haploid induction for production of corresponding haploid versions. Haploid lines, DH lines, and testcrosses were grown in separate but adjacent blocks. Haploid lines were surrounded by a mixture of inbred lines functioning as pollen source. Most haploid plants were male sterile but showed a certain degree of female fertility. Highly significant genetic variation reflected by high heritability coefficients existed in all material sets for all traits. Genetic correlations between haploid and DH lines were moderate to strong (0.5 < genetic correlation coefficient [rg] < 0.9) for early vigor, silking date, plant height, and stover weight per plant. Correlations between DH lines and testcrosses varied from nonsignificant to moderately strong for grain yield but were strong for silking date, plant height, stover yield, and grain moisture content. Silking date, early vigor, and plant height of haploid lines were moderately but significantly associated with grain yield of testcrosses. Somewhat higher estimates were obtained for the corresponding correlations between DH lines and testcrosses. In conclusion, selection for silking date, early vigor, plant height, and stover weight at the haploid level is expected to result in positive correlated genetic gain for various traits not only at the DH but also at the testcross level. Likewise, selection at the DH level may substantially speed up progress in combining ability.

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Section: Discussionmentioning

confidence: 99%

Genetic Correlations among Haploids, Doubled Haploids, and Testcrosses in Maize

2013

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“…Kleiber et al . () reported that the range of haploid fertility is 0%–20% in tropical and temperate maize germplasm. Wu et al .…”

Section: Genome Doublingmentioning

confidence: 99%

Novel technologies in doubled haploid line development

Ren

Trampe

et al. 2017

Plant Biotechnology Journal

118

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Summaryhaploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter‐ and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere‐mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis.

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“…In doubling haploid germplasm, it has been shown that doubling rates of tropical landraces lag behind those of more elite tropical germplasm or Corn Belt materials (Kleiber et al. ). This is consistent with the low doubling rates observed in this experiment.…”

Section: Discussionmentioning

confidence: 99%

Germplasm enhancement of maize: a look into haploid induction and chromosomal doubling of haploids from temperate‐adapted tropical sources

et al. 2016

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The allelic diversity (AD) project of the Germplasm Enhancement of Maize (GEM) programme utilized the double haploid (DH) breeding method to expedite development and release of lines derived from 300 exotic maize races. Using 18 races in this study, differential effects on haploid induction rates (HIRs) and doubling rates (DRs) by the recurrent parents PHB47 and PHZ51, the elevation that the race is traditionally grown at, and by the race itself were addressed in this study. Races from the AD project were grouped by elevation of their origin, high, middle or low altitude. Six races per elevation were randomly selected and backcrossed using both recurrent parents to generate 36 populations. Ten replications were randomized in a complete randomized design for two growing seasons. The recurrent parent effect was significant, with PHB47 having a higher HIR than PHZ51. Effect of elevation was significant with higher HIR associated with low‐elevation origin, and race also proved to be significant. Effects of elevation, recurrent parent and race were not significant for DR.

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Haploid Fertility in Temperate and Tropical Maize Germplasm

Cited by 54 publications

References 21 publications

Genetic Correlations among Haploids, Doubled Haploids, and Testcrosses in Maize

Genetic Correlations among Haploids, Doubled Haploids, and Testcrosses in Maize

Novel technologies in doubled haploid line development

Germplasm enhancement of maize: a look into haploid induction and chromosomal doubling of haploids from temperate‐adapted tropical sources

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