Handheld photoacoustic microscopy to detect melanoma depth in vivo

Zhou, Yong; Xing, Weihong; Maslov, Konstantin; Cornelius, Lynn A.; Wang, Lihong V.

doi:10.1364/ol.39.004731

Cited by 105 publications

(108 citation statements)

References 27 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…1 PAM can provide both anatomical and functional information about biological systems by probing a wide variety of endogenous contrast agents, such as hemoglobin, melanin, DNA/RNA, and lipids. [2][3][4][5][6][7] With tightly focused light, PAM can achieve optical diffraction-limited lateral resolutions down to the submicron scale. 2 This optical-resolution PAM (OR-PAM) enables in vivo functional imaging of blood vessels down to the capillary level.…”

Section: Introductionmentioning

confidence: 99%

Grueneisen relaxation photoacoustic microscopyin vivo

Shi

Hai

et al. 2016

J. Biomed. Opt

Self Cite

View full text Add to dashboard Cite

Abstract. Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically defined axial resolution, but it has been limited to ex vivo demonstrations so far. Here, we present the first in vivo image of a mouse brain acquired with GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was employed to heat absorbing objects. In phantom experiments, an axial resolution of 12.5 μm was achieved, which is sixfold better than the value achieved by conventional optical-resolution PAM. This axial-resolution improvement was further demonstrated by imaging a mouse brain in vivo, where significantly narrower axial profiles of blood vessels were observed. The in vivo demonstration of GR-PAM shows the potential of this modality for label-free and high-resolution anatomical and functional imaging of biological tissues.

show abstract

Section: Introductionmentioning

confidence: 99%

Grueneisen relaxation photoacoustic microscopyin vivo

Shi

Hai

et al. 2016

J. Biomed. Opt

Self Cite

View full text Add to dashboard Cite

show abstract

“…Instead of relying on the diffuse photons scattered from the sample, TE of the detector can also be realized by directing one of the laser fibers at the detector. Possible other important upgrades of the existing dual-modality PA/US microscope would be the use of a compact laser with a higher pulse repetition rate [45] (with a temporal intensity average satisfying necessary safety limits [46]), along with a hand-held design of the scanning system [47]. A 2kHz pulse repetition rate would ensure faster PA/US dual-modality imaging with a 200-fold increase of the A-scan acquisition rate, while the hand-held design of the dual-modality PA/US microscope would ensure more verstile access to the in vivo objects.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous photoacoustic and optically mediated ultrasound microscopy: an in vivo study

Subochev

Orlova

Shirmanova

et al. 2015

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:We propose the use of thermoelastic (TE) excitation of an ultrasonic (US) detector by backscattered laser radiation as a means of upgrading a single-modality photoacoustic (PA) microscope to dualmodality PA/US imaging at minimal cost. The upgraded scanning head of our dual-modality microscope consists of a fiber bundle with 14 output arms and a 32MHz polyvinylidene difluoride (PVDF) detector with a 34 MHz bandwidth (−6 dB level), 12.7 mm focal length, and a 0.25 numerical aperture. A single optical pulse delivered through the fiber bundle to the biotissue being investigated, in combination with a metalized surface on the PVDF detector allows us to obtain both PA and US A-scans. To demonstrate the in vivo capabilities of the proposed method we present the results of bimodal imaging of the brain of a newborn rat, a mouse tail and a mouse tumor.

show abstract

“…It is an excellent avenue for interrogating angiogenesis and blood content in highly aggressive tumors, and shows great potential for molecular imaging [5]. PAI and Photoacoustic Tomography (PAT) have been developing for many years in both clinical and preclinical regimes with applications in skin [6] and breast cancer [7,8], lymph node mapping [9][10][11], and endoscopy [12,13]. These technique relies on absorption of light to create contrast, a phenomenon that is governed by both tissue optical properties and light fluence.…”

Section: Introductionmentioning

confidence: 99%

Quantitative photoacoustic image reconstruction improves accuracy in deep tissue structures

Mastanduno¹,

Gambhir²

2016

Biomed. Opt. Express

View full text Add to dashboard Cite

Photoacoustic imaging (PAI) is emerging as a potentially powerful imaging tool with multiple applications. Image reconstruction for PAI has been relatively limited because of limited or no modeling of light delivery to deep tissues. This work demonstrates a numerical approach to quantitative photoacoustic image reconstruction that minimizes depth and spectrally derived artifacts. We present the first time-domain quantitative photoacoustic image reconstruction algorithm that models optical sources through acoustic data to create quantitative images of absorption coefficients. We demonstrate quantitative accuracy of less than 5% error in large 3 cm diameter 2D geometries with multiple targets and within 22% error in the largest size quantitative photoacoustic studies to date (6cm diameter). We extend the algorithm to spectral data, reconstructing 6 varying chromophores to within 17% of the true values. This quantitiative PA tomography method was able to improve considerably on filtered-back projection from the standpoint of image quality, absolute, and relative quantification in all our simulation geometries. We characterize the effects of time step size, initial guess, and source configuration on final accuracy. This work could help to generate accurate quantitative images from both endogenous absorbers and exogenous photoacoustic dyes in both preclinical and clinical work, thereby increasing the information content obtained especially from deep-tissue photoacoustic imaging studies. Hahn, and A. G. Yodh, "In vivo reflectance measurement of optical properties, blood oxygenation and motexafin lutetium uptake in canine large bowels, kidneys and prostates," Phys. Med. Biol. 47(6), 857-873 (2002)

show abstract

Handheld photoacoustic microscopy to detect melanoma depth in vivo

Cited by 105 publications

References 27 publications

Grueneisen relaxation photoacoustic microscopyin vivo

Grueneisen relaxation photoacoustic microscopyin vivo

Simultaneous photoacoustic and optically mediated ultrasound microscopy: an in vivo study

Quantitative photoacoustic image reconstruction improves accuracy in deep tissue structures

Contact Info

Product

Resources

About