Hand hygiene: virucidal efficacy of a liquid hand wash product against Ebola virus

Abstract

Summary Background Hand washing is an important targeted hygiene intervention for limiting the spread of infectious agents, including the Ebola virus, which continues to re-emerge. We have assessed the virucidal efficacy of a commercially available liquid hand wash product (LHW) for inactivating Ebola virus. Methods The ASTM E1052-11 Standard was used to evaluate the efficacy of an LHW containing the microbicidal active salicylic acid for inactivating Ebola virus - Makona variant suspended in an organic load. Three concentrations (12.5%, 25%, 50%) of three lots of LHW prepared in 440 ppm hard water were evaluated at room temperature for 20, 30, and 60 s contact time. Results A 25% solution of the LHW caused 4.5 log 10 and 4.8 log 10 reduction in Ebola virus titer within 20 and 30 s, respectively. The efficacy of a 12.5% LHW solution was lower (1.9 and 2.0 log 10 reduction in titer within 20 and 30 s, respectively). The efficacy of the 50% LHW solution could not be measured, due to inability to sufficiently neutralize the LHW at the end of exposure. Conclusion These results suggest the potential utility of an appropriately formulated liquid hand wash agent during Ebola virus disease outbreaks for use within healthcare, community, and home settings. Such an LHW should also be effective against other enveloped viruses, such as the pandemic coronavirus SARS-CoV-2.

Sosialisasi Perilaku Hidup Bersih Dan Sehat Dengan Mencuci Tangan Kepada Siswa Sd Santo Bavo Kota Madiun

“…Mencuci tangan merupakan intervensi kebersihan yang ditargetkan untuk membatasi penyebaran agen infeksi. (Cutts et al, 2021) Dengan membiasakan mencuci tangan dengan sabun dapat meminimalkan terjadinya diare hingga 50 persen. (Misroh & Abdul, 2021).…”

Soap, water, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): an ancient handwashing strategy for preventing dissemination of a novel virus

“…Lipid envelope-disrupting hand wash agents, as a class, appear to display efficacy after relatively short contact times. For instance, Ogilvie et al (2020) found that QAC-based products inactivated SAR-CoV-2 in various soil loads within 15 s. Lavelle et al (1989) showed that exposure to a hand wash product containing the phenolic p -chloro- m -xylenol caused concentration-dependent inactivation of human immunodeficiency virus ( Retroviridae ) suspended within a blood matrix within 30 s. The inactivation of Ebola virus Makona variant ( Filoviridae ) within a tripartite soil load (bovine serum albumin, tryptone, and bovine mucin) by a liquid hand wash agent also displayed concentration-dependent inactivation within 20 s contact time ( Cutts et al, 2020a ). Furthermore, disinfectant pre-impregnated wipes containing activated hydrogen peroxide or QAC have been shown to inactivate Ebola virus Makona variant and vesicular stomatitis virus ( Rhabdoviridae ) after a 5-s contact time ( Cutts et al, 2020b ).…”

Soap, Water, and SARS-CoV-2: An Ancient Handwashing Strategy for Preventing Dissemination of a New Virus

“…Lavelle et al (1989) showed that exposure to a hand wash product containing the phenolic pchloro-m-xylenol caused concentration-dependent inactivation of human immunodeficiency virus (Retroviridae) suspended within a blood matrix within 30 seconds. The inactivation of Ebola virus Makona variant (Filoviridae) within a tripartite soil load (bovine serum albumin, tryptone, and bovine mucin) by a liquid handwash agent also displayed concentration-dependent inactivation within 20 seconds contact time (Cutts et al, 2020a). Furthermore, disinfectant preimpregnated wipes containing activate hydrogen peroxide or QAC have been shown to inactivate Ebola virus Makona variant and vesicular stomatitis virus (Rhabdoviridae) after a 5-second contact time Cutts et al, 2020b).…”