Halophyte fatty acids as biomarkers of anthropogenic-driven contamination in Mediterranean marshes: Sentinel species survey and development of an integrated biomarker response (IBR) index

Duarte, Bernardo; Carreiras, João; Pérez-Romero, Jesús Alberto; Mateos‐Naranjo, Enrique; Redondo‐Gómez, Susana; Matos, Ana Rita; Marques, João Carlos

doi:10.1016/j.ecolind.2017.12.050

Cited by 44 publications

(30 citation statements)

References 36 publications

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“…The ability to adjust membrane lipid fluidity by changing levels of unsaturated fatty acids is a feature of stress acclimating plants maintaining a suitable environment for the function of integral proteins, such as the photosynthetic machinery 23 . Thus, we suggest that after pathogen challenge, the increase on membrane fluidity on the resistant genotype may be crucial to avoid membrane damage that represents inevitable effects on the energy transduction pathways and primary productivity 24 . Indeed, we have shown that a ROS burst occurs in Regent at 6 hpi 8 .…”

Section: Resultsmentioning

confidence: 99%

“…In addition to the traditional univariate comparisons between fatty acid contents of each genotype, we also performed the evaluation of the whole lipid profile as one. Thus, the data was analysed using a multivariate approach that already proved to be an efficient tool to evaluate the fatty acid profile of plant ecotypes 24 . All statistical analysis were conducted using Primer 6 software 83 .…”

Section: Methodsmentioning

confidence: 99%

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The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola

Laureano

Figueiredo

Cavaco

et al. 2018

Sci Rep

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Grapevine downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most important diseases in modern viticulture. The search for sustainable disease control measure is of extreme importance, thus becoming imperative to fully characterize the mechanisms leading to an incompatible interaction. We have previously shown that lipid signalling events play an important role in grapevine’s response to this pathogen, namely through changes in linolenic acid content, lipid peroxidation and jasmonic acid synthesis. Here, we have characterized the modulation of lipid metabolism in leaves from two V. vinifera cultivars (resistant and susceptible to P. viticola) in the first hours after pathogen inoculation. Prior to pathogen inoculation both genotypes present an inherently different fatty acid composition that is highly modulated in the resistant genotype after pathogen challenge. Such changes involve modulation of phospholipase A activity suggesting that the source of lipids mobilized upon pathogen infection are the chloroplast membranes. This work thus provides original evidence on the involvement of lipid signalling and phospholipases in grapevine immune responses to pathogen infection. The results are discussed considering the implications on the plant’s physiological status and the use of discriminating lipid/fatty acids pattern in future selection procedures of cultivars.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola

Laureano

Figueiredo

Cavaco

et al. 2018

Sci Rep

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“…The data obtained from the Kautsky plots, pigment, and fatty acid profiles, were used as the basis for the construction of the respective resemblance matrixes based on the Euclidean distances between samples. To classify and separate the different treatment groups, statistical multivariate models based on the Kautsky plot, pigment composition, and fatty acid profile variable were generated using Canonical Analysis of Principal Coordinates (CAP), through the non-parametric multivariate analysis packages in Primer 6 software as described previously in other works [12,45,68,69].…”

Section: Discussionmentioning

confidence: 99%

Glyphosate-Based Herbicide Toxicophenomics in Marine Diatoms: Impacts on Primary Production and Physiological Fitness

et al. 2020

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Glyphosate is the main active component of the commercial formulation Roundup®, the most widely used chemical herbicide worldwide. However, its potential high toxicity to the environment and throughout trophic webs has come under increasing scrutiny. The present study aims to investigate the application of bio-optical techniques and their correlation to physiological and biochemical processes, including primary productivity, oxidative stress, energy balance, and alterations in pigment and lipid composition in Phaeodactylum tricornutum, a representative species of marine diatoms, using the case study of its response to the herbicide glyphosate-based Roundup® formulation, at environmentally relevant concentrations. Cultures were exposed to the herbicide formulation representing effective glyphosate concentrations of 0, 10, 50, 100, 250, and 500 μg L−1. Results showed that high concentrations decreased cell density; furthermore, the inhibition of photosynthetic activity was not only caused by the impairment of electron transport in the thylakoids, but also by a decrease of antioxidant capacity and increased lipid peroxidation. Nevertheless, concentrations of one of the plastidial marker fatty acids had a positive correlation with the highest concentration as well as an increase in total protein. Cell energy allocation also increased with concentration, relative to control and the lowest concentration, although culture growth was inhibited. Pigment composition and fatty acid profiles proved to be efficient biomarkers for the highest glyphosate-based herbicide concentrations, while bio-optical data separated controls from intermediate concentrations and high concentrations.

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“…1 and Table 1) assessed in a first census by visual inspection (Duarte et al, 2017b). Additionally, from this same sampling campaign resulted a previous study that also showed that these patches show increasing degrees of photobiological maturation (Duarte et al, 2018b) (Table 1), as shown by its increasing quantum yield values (Fv/Fm). The 2008 collapse event and the following irregular recovery since 2009 (Materatski et al, 2016), lead to the re-appearance of patches at different developmental stages.…”

Section: Sample Collectionmentioning

confidence: 74%

Dwarf eelgrass (Zostera noltii) leaf fatty acid profile during a natural restoration process: Physiological and ecological implications

Duarte

Matos

Pedro

et al. 2019

Ecological Indicators

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A B S T R A C TSeagrass beds are among the most relevant ecosystem engineers, providing essential ecosystem services for the surrounding coastal communities. Alongside, these ecosystems are among the most threaten in the world, and thus several restoration projects have been developed in the past years. Seagrasses are important sources of essential fatty acids (FA) for animals, which are unable to synthetize them. During the seagrass growth and photochemical maturation, there is a membrane remodelling, where some fatty acids are synthetized. When analysing the FA changes at different development stages, one of the first noticeable changes is the increase in C16:1t (trans-hexadecenoic acid), associated to an increase in the chloroplast membrane fluidity, essential for the efficient energy transduction processes to occur in the thylakoids. Also, interesting to observe are the high levels of omega-3 and -6 (30-43% and 18-31% of total fatty acid content, respectively) present in this seagrass, reinforcing the nutritional value of this species as source of essential fatty acids for the primary consumers. Additionally, it is possible to observe that in the more mature plants there is a high leaf concentration of C18:3. Recent reports suggest that C18:3 can act as a direct scavenger of reactive oxygen species (ROS), indicating a lower stress level, as suggested by the higher photochemical efficiency previously observed. Moreover, it is also interesting to observe that total long-chain polyunsaturated fatty acids (LC-PUFAs) content show a significant increase with the biomass development. These LC-PUFAs are not produced by higher plants and their production in animals occurs at low rates, suggesting they may have as origin the microalgae in the grass surface, adding another important ecosystem service to these prairies, as support for microalgae development and carriers of LC-PUFAs into the food web.This membrane remodelling appears to be on the basis of the photochemical maturation of these seagrasses observed in previous studies and can be used as potential and efficient tool to monitor the development stage of the prairies and its physiological status in future restoration processes. Moreover, it becomes evident that highly developed seagrass beds are crucial food source providers in terms of essential fatty acids to the estuarine heterotrophic life.

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Halophyte fatty acids as biomarkers of anthropogenic-driven contamination in Mediterranean marshes: Sentinel species survey and development of an integrated biomarker response (IBR) index

Cited by 44 publications

References 36 publications

The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola

The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola

Glyphosate-Based Herbicide Toxicophenomics in Marine Diatoms: Impacts on Primary Production and Physiological Fitness

Dwarf eelgrass (Zostera noltii) leaf fatty acid profile during a natural restoration process: Physiological and ecological implications

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