Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China

Xu, Xue-Wei; Wu, Yue‐Hong; Zhou, Zhen; Wang, Chunsheng; Zhou, Yu‐Guang; Zhang, Huibin; Wang, Yong; Wu, Min

doi:10.1099/ijs.0.65022-0

Cited by 116 publications

(75 citation statements)

References 33 publications

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“…The 16S rRNA gene was amplified and analysed as described previously (Xu et al, 2007b). PCR products were cloned into vector pMD 19-T (TaKaRa) and then sequenced to determine the almost-complete 16S rRNA gene sequence.…”

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Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

Wang

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39 T , was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C 16 : 1 v7c/iso-C 15 : 0 2-OH, C 16 : 0 , C 18 : 1 v7c, C 12 : 0 3-OH, C 17 : 1 v8c and C 17 : 0 . The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Pseudoalteromonas. Strain LMEB 39 T exhibited the closest phylogenetic affinity to Pseudoalteromonas byunsanensis JCM 12483 T (97.4 % sequence similarity). The DNA-DNA reassociation values between strain LMEB 39 T and P. byunsanensis JCM 12483 T and Pseudoalteromonas undina DSM 6065 T (97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39 T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas lipolytica sp. nov. is proposed; the type strain is LMEB 39 T (5CGMCC 1.8499 T 5JCM 15903 T ).The genus Pseudoalteromonas was proposed by Gauthier et al. (1995) and represents a clade of marine bacteria grouped by their phylogenetic relationships based on 16S rRNA gene sequences. Members of the genus Pseudoalteromonas are Gram-negative, strictly aerobic, rod-shaped cells. They are motile by a single polar flagellum and have a chemoheterotrophic metabolism (Bowman & McMeekin, 2005). At the time of writing, the genus Pseudoalteromonas comprises 34 species with validly published names (Gauthier et al., 1995;Ivanova et al., 1996Ivanova et al., , 1998Ivanova et al., , 2000Ivanova et al., , 2001 Ivanova et al., , 2002a Ivanova et al., , b, c, d, 2004Bozal et al., 1997; Euzéby, 1997;Bowman, 1998; Holmström et al., 1998; Sawabe et al., 2000;Venkateswaran & Dohmoto, 2000;Egan et al., 2001;Isnansetyo & Kamei, 2003; Romanenko et al., 2003a, b;Lau et al., 2005;Park et al., 2005;Nam et al., 2007;Al Khudary et al., 2008). Some strains produce yellow, red, violet or melanin-like pigments and have the ability to form antibiotic substances (Bowman, 2007). Here, a novel non-pigmented Pseudoalteromonas strain isolated from the Yangtze River estuary was studied using a polyphasic approach.A seawater sample was collected in October 2007 from the Yangtze River estuary (31u 009 040 N 122u 379 260 E) near the East China Sea at a depth of 21 m (temperature 21 u C, salinity 30.5 %). The sample was stored in darkness until micro-organisms were isolated in July 2008. Approximately 80 ml seawater was plated on halophilic medium (HM) containing 10 % (w/v) NaCl by using a te...

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Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

Wang

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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“…The 16S rRNA gene was amplified and analysed as described previously (Xu et al, 2007b). The sequence was compared with closely related sequences of reference organisms from the FASTA and EZTAXON services (Chun et al, 2007).…”

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Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

Wang

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae A Gram-negative, aerobic, neutrophilic, coccoid bacterium, strain E4A9 T , was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region. 16S rRNA gene sequence analysis showed that the isolate was related to the type strain of Altererythrobacter epoxidivorans (96.0 % sequence similarity). Lower 16S rRNA gene sequence similarities were observed with other members of the genera Altererythrobacter (94.7 %), Erythrobacter (94.0-95.4 %), Erythromicrobium (94.8 %) and Porphyrobacter (94.6-95.1 %) of the family Erythrobacteraceae. Phylogenetic analysis including all described species of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a distinct phylogenetic lineage with the family Erythrobacteraceae. Chemotaxonomic analysis revealed ubiquinone-10 as the predominant respiratory quinone, anteiso-C 15 : 0 , iso-C 14 : 0 and iso-C 15 : 0 as major fatty acids, and phosphatidylglycerol as the major polar lipid. The DNA G+C content was 71.5 mol%. The isolate contained carotenoids, but no bacteriochlorophyll a. On the basis of phenotypic and genotypic data presented in this study, strain E4A9 T represents a novel species in a new genus in the family Erythrobacteraceae for which the name Croceicoccus marinus gen. nov., sp. nov. is proposed; the type strain is E4A9 T (5CGMCC 1.6776 T 5JCM 14846 T ).The family Erythrobacteraceae (order Sphingomonadales, class Alphaproteobacteria) was proposed by Lee et al. (2005) based on a comprehensive phylogenetic analysis and, at present, it comprises four recognized genera: Altererythrobacter (Kwon et al., 2007), Erythrobacter (Shiba & Simidu, 1982), Erythromicrobium (Yurkov et al., 1994) and Porphyrobacter (Fuerst et al., 1993).Members of the family Erythrobacteraceae are aerobic and produce pink, orange or yellow pigments. Ubiquinone-10 is the major respiratory quinone. Most species in the family have been isolated from various aquatic environments such as freshwater, seawater, marine mats or sediment, a hot spring, seaweed, a starfish and coral (Shiba & Simidu, 1982; Fuerst et al., 1993;Yurkov et al., 1994; Hanada et al., 1997; Denner et al., 2002; Hiraishi et al., 2002;Rainey et al., 2003;Yoon et al., 2003Yoon et al., , 2004a Yoon et al., , b, 2005a Yoon et al., , b, 2006Ivanova et al., 2005;Kwon et al., 2007). This study focuses on the description of strain E4A9 T , isolated from a deep-sea sediment sample. Based on the taxonomic data, it is proposed that this strain be included in a new genus within the family Erythrobacteraceae.The deep-sea sediment samples were collected by a multicorer from the East Pacific polymetallic nodule region (8 u 229 380 N 145 u 239 560 W) at a depth of Abbreviation: BChl a, bacteriochlorophyll a.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain E4A9 T is EF62399...

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“…The 16S rRNA gene was amplified and analyzed as described previously (Xu et al, 2007). PCR products were cloned into vector pMD 19-T (TaKaRa) and then sequenced to determine the almost-complete sequence of 16S rRNA gene.…”

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Aquaticitalea lipolytica gen. nov., sp. nov., isolated from Antarctic seawater

Xamxidin

Jian

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, rod-shaped bacterium, designated Ar-125 T , was isolated from Antarctic seawater. It produced carotenoid-like pigments and did not produce Bchl a. Ar-125 T was positive for hydrolysis of DNA, aesculin, gelatin, starch, Tween 40 and Tween 60. The sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, one unidentified glycolipid and two unidentified lipids. The principal fatty acids were branched-chain fatty acids, including iso-C 15 : 0, iso-C 15 : 1 G, summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 !7c), iso-C 16 : 0, iso-C 17 : 0 3-OH, iso-C 16 : 0 3-OH and iso-C 15 : 0 3-OH, as well as C 15 : 0 . The genomic DNA G+C content was 31.8 mol%. On the basis of 16S rRNA gene sequence analysis, Ar-125 T is closely related to the species of the genera Bizionia (with 16S rRNA gene pairwise sequence similarity of 93.7-96.5 %), Formosa(94.3-95.8 %), Gaetbulibacter(94.2-95.7 %), Geojedonia(95.5 %), Gelidibacter (93.3-95.4 %), Meridianimaribacter(95.3 %) and Psychroserpens (94.8-95.3 %), of the family Flavobacteriaceae. Phylogenetic analysis indicated that it represented an independent lineage and that the closest relatives were members of the genus Gelidibacter. Differential phenotypic properties and chemotaxonomic differences, together with phylogenetic distinctiveness, revealed that Ar-125 T could be differentiated from members of closely related genera. Therefore, it is proposed that Ar-125 T represents a novel species in a new genus, for which the name Aquaticitalea lipolytica gen. nov., sp. nov. (type strain Ar-125 T =CGMCC 1.15295 T =JCM 30876 T ) is proposed.

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Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China

Cited by 116 publications

References 33 publications

Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary

Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

Aquaticitalea lipolytica gen. nov., sp. nov., isolated from Antarctic seawater

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