Halobacterium litoreum sp. nov., isolated from a marine solar saltern

Abstract

Halophilic archaeal strain ZS-54-S2 was isolated from Zhoushan marine solar saltern, China. Cells were rod-shaped, Gram-stain-negative and formed red-pigmented colonies on an agar plate. Strain ZS-54-S2 was able to grow at 20-50 °C (optimum 35 °C), at 1.7-4.8 M NaCl (optimum 3.9 M), at 0.005-1.0 M MgCl2 (optimum 0.05 M) and at pH 5.0-9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two glycolipids, which were chromatographically identical to sulfated galactosyl mannosyl galactofuranosyl glucosyl diether and galactosyl mannosyl glucosyl diether, and an unidentified glycolipid, which was chromatographically identical to one detected in Halobacterium salinarum ATCC 33171. The 16S rRNA gene and rpoB' gene of strain ZS-54-S2 were phylogenetically related to the corresponding genes of Halobacterium noricense JCM 15102 (97.5 % and 90.6 % relatedness, respectively), Halobacterium jilantaiense CGMCC 1.5337 (96.9 and 91.2 %), Halobacterium rubrum CGMCC 1.12575 (96.8 and 90.3 %) and Halobacterium salinarum CGMCC 1.1958 (96.5 and 88.4 %). The DNA G+C content of strain ZS-54-S2 was 66.7 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-54-S2 (=CGMCC 1.12562=JCM 30038) represents a new species of Halobacterium, for which the name Halobacteriumlitoreum sp. nov. is proposed.

Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)

“…Haloarcula [ 39 , 40 ] and Halobacterium [ 41 , 42 ] genera belong to the Halobacteriaceae family within the Halobacteriales order. Currently, ten recognized species are included in the Haloarcula genus: H. amylolytica [ 43 ], H. argentinensis [ 44 ], H. vallismortis [ 45 ], H. marismortui [ 46 ], H. hispanica [ 47 ], H. japonica [ 48 ], H. quadrata [ 49 ], H. sinaiiensis [ 50 ], H. Salaria and H. Tradensis [ 51 ]; while to date, five species comprise the Halobacterium genus: H. Jilantaiense [ 52 ], H. litoreum [ 53 ], H. noricense [ 54 ], H. rubrum [ 55 ] and H. salinarum [ 56 ]. Several strains of these species have been described due to the divergence of their 16S RNA coding gene sequences, as is the case of the two strains proposed here, which have been designated as Haloarcula hispanica HM1 and Halobacterium salinarum HM2.…”

Halobacterium hubeiense sp. nov., a haloarchaeal species isolated from a bore core drilled in Hubei Province, PR China

“…At present, the genus Halobacterium is classified as the type genus of the family Halobacteriaceae , order Halobacteriales , within the class Halobacteria [ 10 11 ]. Currently, this genus includes eight species: Halobacterium salinarum (type species of the genus) [ 12 13 ], Halobacterium bonnevillei [ 14 ], Halobacterium jilantaiense [ 15 ], Halobacterium litoreum [ 16 ], Halobacterium noricense [ 13 ], Halobacterium rubrum [ 17 ], Halobacterium wangiae [ 18 ], and Halobacterium zhouii [ 18 ]. Species of Halobacterium are Gram-stain-negative, motile, rod-shaped cells, and their colonies are red or pink pigmented.…”

Extremely halophilic strains of Halobacterium salinarum as a potential starter culture for fish sauce fermentation

“…The isolation and identification of halophilic archaea in various hypersaline environments, such as salted foods, saline soil, marine solar saltern, and salt lakes, have been well reported (Han et al., 2015, 2019; Lü et al., 2017; Xu et al., 2015). Halorubrum , Halobacterium , Hallobellus , Natronomonas , Haloplanus , Halonotius , Halomarina , and Haloarcula were found to be predominant archaea in commercial sea salt products (Satari et al., 2021).…”