1991
DOI: 10.1128/jb.173.3.955-960.1991
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Halobacterium halobium strains lysogenic for phage phi H contain a protein resembling coliphage repressors

Abstract: DNA-binding proteins such as bacteriophage repressors belong to the helix-turn-helix family. Ionic interactions drive DNA binding, which means that repressors bind DNA most tightly at low salt concentrations. This raises the question of how gene expression might be regulated in obligate halophiles, which maintain internal salt concentrations of about 5 M. As a model system we have investigated the phage OH, which infects the archaebacterium Halobacterium halobium. Previous genetic data and transcriptional mapp… Show more

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Cited by 42 publications
(28 citation statements)
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“…By comparison, the palindromic sequence in the M. maripaludis nifH operator starts just two nucleotides from the transcription start, and repressor binding there could also interfere with some step in transcriptional initiation. We are aware of one other example of transcriptional regulation by repression in Archaea; a repressor present in phage H lysogens of Halobacterium halobium apparently functions by binding to palindromic sites immediately upstream of the TATA box for a lytic gene (27,28). However, despite similarities between bacterial repression and the observations made to date in Archaea, the picture is still incomplete and it would be premature to suggest that repression in Archaea occurs by a bacterial mechanism.…”
Section: Resultsmentioning
confidence: 78%
See 1 more Smart Citation
“…By comparison, the palindromic sequence in the M. maripaludis nifH operator starts just two nucleotides from the transcription start, and repressor binding there could also interfere with some step in transcriptional initiation. We are aware of one other example of transcriptional regulation by repression in Archaea; a repressor present in phage H lysogens of Halobacterium halobium apparently functions by binding to palindromic sites immediately upstream of the TATA box for a lytic gene (27,28). However, despite similarities between bacterial repression and the observations made to date in Archaea, the picture is still incomplete and it would be premature to suggest that repression in Archaea occurs by a bacterial mechanism.…”
Section: Resultsmentioning
confidence: 78%
“…29-31), and evidently in H. halobium H as well (27), nearby copies of similar palindromes allow higher order cooperative interactions to occur due to repressor tetramers. In the M. maripaludis nifH promoter region, however, although a second palindrome exists, no evidence for any role was obtained.…”
Section: Resultsmentioning
confidence: 99%
“…and S.D., unpublished observations). Previously, one halophage HTH protein was shown to bind DNA in band-shift assays (26). Finally, the redox-sensing PAS͞PAC region was also present in Bat (3,11).…”
Section: Identification Of Purple Membrane Regulon Genesmentioning
confidence: 99%
“…In one of the two kingdoms, the Euryarchaeota (1), two phage/host systems have been studied in some molecular detail: the temperate phage DH of Halobacterium halobium (4)(5)(6) and the transducing phage TM1 of Methanobacterium thermoautotrophicum (7,8). In the other kingdom of the archaeal domain, the Crenarchaeota (1), comprising extreme thermophiles, a family of viruses of the anaerobic host Thermoproteus tenax, TTV1 through TTV4 (2,9,10), is of limited usefulness, because no plaque assay is available.…”
mentioning
confidence: 99%