2001
DOI: 10.1073/pnas.051632498
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Genomic and genetic dissection of an archaeal regulon

Abstract: The extremely halophilic archaeon Halobacterium sp. NRC-1 can grow phototrophically by means of light-driven proton pumping by bacteriorhodopsin in the purple membrane. Here, we show by genetic analysis of the wild type, and insertion and double-frame shift mutants of Bat that this transcriptional regulator coordinates synthesis of a structural protein and a chromophore for purple membrane biogenesis in response to both light and oxygen. Analysis of the complete Halobacterium sp. NRC-1 genome sequence showed t… Show more

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Cited by 64 publications
(77 citation statements)
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“…While the blh-crtY and atp operons are both involved in ATP synthesis, it is not clear how this benefits the halophiles, given that these genes are regulated in an exactly opposing manner under changing oxygen conditions ). Consistent with this and prior studies (Baliga et al 2001(Baliga et al , 2002Bonneau et al 2007), growth-associated regulation of crtY-blh transcript was also anti-correlated with atpIKECFAB (r ; À0.8) and highly correlated with other TUs of phototrophy genes including blp, bat-brp, and bop (r ; 0.9). Interestingly, our data reveal that the relatively new promoter of atpD is gaining similar regulatory control as that of the parent operon.…”
Section: Lineage Specific Reorganizations Of the Atp Operonsupporting
confidence: 76%
“…While the blh-crtY and atp operons are both involved in ATP synthesis, it is not clear how this benefits the halophiles, given that these genes are regulated in an exactly opposing manner under changing oxygen conditions ). Consistent with this and prior studies (Baliga et al 2001(Baliga et al , 2002Bonneau et al 2007), growth-associated regulation of crtY-blh transcript was also anti-correlated with atpIKECFAB (r ; À0.8) and highly correlated with other TUs of phototrophy genes including blp, bat-brp, and bop (r ; 0.9). Interestingly, our data reveal that the relatively new promoter of atpD is gaining similar regulatory control as that of the parent operon.…”
Section: Lineage Specific Reorganizations Of the Atp Operonsupporting
confidence: 76%
“…The bacterio-opsin gene bop displayed the highest up-regulation (15-fold) of all ORFs. The strong differential expression of the bop gene is consistent with its regulation by the oxygenor redox-sensing activator Bat (2,3,40,41). Genes involved in aerobic respiration in NRC-1 were not down-regulated in the absence of oxygen.…”
Section: Fig 3 Rt-pcr Analysis Of the Dms Operon Insupporting
confidence: 48%
“…strain NRC-1 under anaerobic conditions. Previous work utilized a combination of genetic and genomic studies to establish the purple membrane regulon, which leads to coordinate synthesis of bacterio-opsin and its chromophore, retinal, to allow for light-driven proton pumping (2,8,40,41). The previous work led to the identification of a novel positive regulator, Bat, which controls the expression of the bacterio-opsin (bop) gene, as well as the first and last steps in the retinal biosynthetic pathway.…”
Section: Discussionmentioning
confidence: 99%
“…Recent success in solving the structure of the large ribosomal subunit from a related halophile, Haloarcula marismortui (Ban et al 2000), genomic and genetic dissection of the archaeal regulon for purple membrane synthesis in Halobacterium NRC-1 (Baliga et al 2001), and reverse genetic analysis of carotenoid and retinal biosynthesis (Peck et al 2001) further solidify halophiles as viable and indispensable model systems in both prokaryotic and eukaryotic fields. Undoubtedly, on-going work using genetic arrays, gene knockouts, and further computational analysis will yield insights into life in extreme environments generally and adaptation to hypersaline environments specifically.…”
Section: Discussionmentioning
confidence: 99%