Halobacteria as producers of polyhydroxyalkanoates

Rodrı́guez-Valera, Francisco; Lillo, José Antonio García

doi:10.1111/j.1574-6968.1992.tb05836.x

Cited by 69 publications

(36 citation statements)

References 22 publications

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“…mediterannei, an organism originally isolated from ponds of a solar saltern near Santa Pola at the Iberian coast of the Mediterranean sea, already underlined the positive effect of the used unusual culture medium, containing more than 20 % NaCl, to prevent contamination by alien microbes [44]. The high osmotic pressure of this strain was also used to release PHA granules from the surrounding cell mass via a simple approach: salt concentration below 10 % already caused partial cell lysis; exposure to hypotonic media (distilled water) completely disrupts the cells due to their high inner-osmotic pressure, and sets free the storage material (PHA granules).…”

Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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The article reviews the current state of knowledge of the production of polyhydroxyalkanoate (PHA) biopolyesters under extreme environmental conditions.Although PHA production by extremophiles is not realized yet at industrial scale, significant PHA accumulation under high salinity or extreme pH-or temperature conditions was reported for diverse representatives of the microbial domains of both Archaea and Bacteria. Several mechanisms were proposed to explain the mechanistic role of PHA and their monomers as microbial cell-and enzyme protective chaperons and the factors boosting PHA biosynthesis under environmental stress conditions. The potential of selected extremophile strains, isolated from extreme environments like glaciers, hot springs, saline brines, or from habitats highly polluted with heavy metals or solvents, for efficient future PHA production on an industrially relevant scale is assessed based on the basic data available in the scientific literature. The article reveals that, beside the needed optimization of other cost-decisive factors like inexpensive raw materials or efficient downstream processing, the application of extremophile production strains can drastically safe energy costs, are easily accessible towards long-term cultivation in chemostat processes, and therefore might pave the way towards cost-efficient PHA production, even combined with safe disposal of industrial waste streams. However, further challenges have still to be overcome in terms of strain improvement and process engineering aspects.

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Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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“…The background of this metabolic particularity was only recently elucidated and understood on the enzymatic and genetic level [37][38][39] . Regarding EPS biosynthesis, the strain is known to excrete an anionic EPS of high molar mass 8,16,40 , resulting in a typical mucous character of colonies grown on solid medium (see also Fig. 2).…”

Section: Pha and Eps From H Mediterraneimentioning

confidence: 99%

“…Production of these valued compounds was described starting from carbon sources like glucose 16 , hydrolyzed lactose from dairy waste 9,10 , starchy residues 17,18 , vinasse 19 , rice-based ethanol stillage 20 , or glycerol from biodiesel production 21 . Already in 1995, the high potential of this organism to be implemented in "White Biotechnology", especially for bioplastic production, was discussed 22 , whereas only a few years ago, the application of the strain for PHA production explicitly from different agro-industrial waste streams was suggested by several authors 9,10,[17][18][19][20][21] .…”

Section: Biotechnological Potential Of the Strain Haloferax Mediterraneimentioning

confidence: 99%

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Koller

Chiellini

Braunegg

2015

Chem.Biochem.Eng.Q.

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The halobacterium Haloferax mediterranei was used to study the production of two types of biopolymers: The biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was accumulated as intracellular granules, whereas an extracelluar polysaccharide was excreted in parallel to biopolyester synthesis.After production, microbial re-use and degradation of these polymers under different conditions were investigated to assess the requirements for handling the product-rich fermentation broth prior to the downstream processing for product recovery. Degradation kinetics of the polymers and the impact of different storage conditions on molar mass of PHBV were studied.It turned out that the biotechnological fermentation process can be run without any sterility precautions. No major product losses were observed without pasteurization of fermentation broth after the stop of fermentation. In addition, neither PHBV nor EPS are re-utilized by the cells for biomass formation even if the culture is maintained under conditions of carbon starvation for an extended time.

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“…Polyhydroxybutyrate (PHB) is the best-studied example of biodegradable polyesters belonging to the group of polyhydroxyalkanoates (PHAs), which are synthesized by many bacteria and archaea as intracellular carbon and energy reserves (1,40,43). In the last decades, these biopolymers have received great attention due to their properties which resemble those of conventional petrochemical-based polymers (49).…”

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confidence: 99%

Poly(3-Hydroxybutyrate) Production from Glycerol by Zobellella denitrificans MW1 via High-Cell-Density Fed-Batch Fermentation and Simplified Solvent Extraction

Ibrahim

Steinbüchel

2009

Appl Environ Microbiol

132

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Industrial production of biodegradable polyesters such as polyhydroxyalkanoates is hampered by high production costs, among which the costs for substrates and for downstream processing represent the main obstacles. Inexpensive fermentable raw materials such as crude glycerol, an abundant by-product of the biodiesel industry, have emerged to be promising carbon sources for industrial fermentations. In this study, Zobellella denitrificans MW1, a recently isolated bacterium, was used for the production of poly(3-hydroxybutyrate) (PHB) from glycerol as the sole carbon source. Pilot-scale fermentations (42-liter scale) were conducted to scale up the high PHB accumulation capability of this strain. By fed-batch cultivation, at first a relatively high cell density (29.9 ؎ 1.3 g/liter) was obtained during only a short fermentation period (24 h). However, the PHB content was relatively low (31.0%

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Halobacteria as producers of polyhydroxyalkanoates

Cited by 69 publications

References 22 publications

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Poly(3-Hydroxybutyrate) Production from Glycerol by Zobellella denitrificans MW1 via High-Cell-Density Fed-Batch Fermentation and Simplified Solvent Extraction

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