Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep

Abstract

The genus Halobacillus, with the type species Halobacillus halophilus, was established and described by Spring and co-workers in 1996 (Claus et al., 1983;Spring et al., 1996). So far, nine species of this genus have been identified (Amoozegar et al., 2003a, b;Yoon et al., 2003Yoon et al., , 2004Yoon et al., , 2005Liu et al., 2005). The increasing number of publications on bio-applications and other aspects of Halobacillus and the large number of 16S rRNA gene sequences deposited in databases for unidentified species reflect the wide distribution of these bacteria and their considerable scientific interest (Burja et al., 1999;Pinar et al., 2001;Yang et al., 2002; Rivadeneyra et al., 2004). In our investigations of the diversity of halophilic bacteria in various habitats over the last few years, we obtained strains IS-Hb4 T and IS-Hb7 T in an endemic population from a carbonate rock collected at a deep-sea methane seep at Kuroshima Knoll, south-western Japan. Living in the special environment of low temperature, high pressure and low oxygen availability, these bacteria possess several features that are different from those of described species that would be considered evidence of interesting adaptation.Samples from which the bacteria were isolated, carbonate rocks authentically formed in a methane seep of the Kuroshima Knoll, located at 642 m depth at 24 u 07.89 N 124 u 11.29 E (Fujikura et al., 2003;Inagaki et al., 2004), were collected with the remotely operated vehicle Dolphin 3K, operated by the Japan Agency for Marine Earth Science and Abbreviation: CZE, capillary zone electrophoresis.

Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain

“…T was quite similar to the complex lipid patterns reported for recognized Halobacillus species, with the ; 2, H. halophilus (data from the present study and from Claus et al, 1983;Spring et al, 1996); 3, H. trueperi (Spring et al, 1996); 4, H. litoralis (Spring et al, 1996); 5, H. salinus (Yoon et al, 2003); 6, H. karajensis (Amoozegar et al, 2003); 7, H. locisalis (Yoon et al, 2004); 8, H. dabanensis (Liu et al, 2005); 9, H. aidingensis (Liu et al, 2005); 10, H. yeomjeoni (Yoon et al, 2005); 11, H. profundi (Hua et al, 2007); 12, H. kuroshimensis (Hua et al, 2007). +, Positive; 2, negative; W, weakly positive; V, variable; ND, no data available.…”

“…At the time of writing, the genus comprises 13 species with validly published names, with the addition of Halobacillus salinus (Yoon et al, 2003), H. karajensis (Amoozegar et al, 2003), H. locisalis (Yoon et al, 2004), H. yeomjeoni (Yoon et al, 2005), H. dabanensis and H. aidingensis (Liu et al, 2005), H. profundi and H. kuroshimensis (Hua et al, 2007), H. campisalis (Yoon et al, 2007) and H. faecis .…”

Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake

“…T from the majority of the species included in the genus Halobacillus, which have been described as having a cell-wall peptidoglycan type based on L-Orn-D-Asp (Amoozegar et al, 2003;An et al, 2007;Hua et al, 2007;Liu et al, 2005;Spring et al, 1996;Yoon et al, 2003Yoon et al, , 2004Yoon et al, , 2005. H. campisalis is the sole species belonging to the genus Halobacillus with a cellwall peptidoglycan type based on meso-diaminopimelic acid (Yoon et al, 2007); however, this species is distantly related to our novel isolate (96.5 % gene sequence similarity).…”

Sediminibacillus halophilus gen. nov., sp. nov., a moderately halophilic, Gram-positive bacterium from a hypersaline lake

“…T from the majority of the species of the genus Halobacillus, which have been described as having a cell-wall type based on L-Orn-DAsp (Spring et al, 1996;Amoozegar et al, 2003;Yoon et al, 2003Yoon et al, , 2004Yoon et al, , 2005bLiu et al, 2005;Hua et al, 2007;An et al, 2007b). Halobacillus campisalis is the only Halobacillus species with a cell-wall type based on meso-diaminopimelic acid (Yoon et al, 2007); however, this species is related distantly to our isolate (95.6 % gene sequence similarity).…”