Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake

Abstract

A Gram-positive, moderately halophilic bacterium, designated strain HS286T , was isolated from water of the hypersaline Lake Howz-Soltan in Iran. Cells were strictly aerobic, rod-shaped, motile and able to produce ellipsoidal endospores at a central-subterminal position in swollen sporangia. Isolate HS286 T grew in a complex medium supplemented with 1-15 % (w/v) NaCl, with optimum growth at 8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain HS286 T was closely related to Thalassobacillus devorans G-19.1 T (99.4 % gene sequence similarity). The other closest species were Halobacillus yeomjeoni MSS-402 T (96.9 %) and other species of the genus Halobacillus (with 96.7-93.5 % similarity). Strain HS286 T had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the respiratory isoprenoid quinone. The major fatty acids were anteiso-C 15 : 0 (43.8 %), iso-C 16 : 0 (21.4 %), iso-C 14 : 0 (9.4 %), anteiso-C 17 : 0 (8.7 %) and iso-C 15 : 0 (7.0 %) and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids and a glycolipid. The DNA G+C content was 43.0 mol%. All of these features confirmed the placement of isolate HS286 T within the genus Thalassobacillus. However DNA-DNA hybridization between strain HS286 T and the only recognized species of the genus Thalassobacillus, T. devorans G-19

Thalassobacillus hwangdonensis sp. nov., isolated from a tidal flat sediment

“…Strain AD-1 T contained large amounts of branched fatty acids: the major fatty acid was iso-C 15 : 0 (48.9 %), and significant amounts (.10 % of total fatty acids) of iso-C 17 : 0 and anteiso-C 15 : 0 were detected ( Table 2). The cellular fatty acid profiles determined in this study for T. devorans DSM 16966 T and T. cyri HS286 T were essentially the same as those determined previously, although there were differences in the contents of some fatty acids (García et al, 2005;Sanchez-Porro et al, 2009). The fatty acid profiles of the three strains were similar although there were differences in the proportions of the major fatty acids.…”

“…The predominant menaquinone detected in strain AD-1 T was MK-7 (peak area ratio of approximately 95 %). The cell-wall peptidoglycan and predominant menaquinone type of strain AD-1 T were the same as those of the two reference strains (García et al, 2005;Sanchez-Porro et al, 2009). Strain AD-1 T contained large amounts of branched fatty acids: the major fatty acid was iso-C 15 : 0 (48.9 %), and significant amounts (.10 % of total fatty acids) of iso-C 17 : 0 and anteiso-C 15 : 0 were detected ( Table 2).…”

“…The genus Thalassobacillus was first described by García et al (2005) with the description of a single species, Thalassobacillus devorans. Subsequently, a second species of the genus has been described, Thalassobacillus cyri (Sanchez-Porro et al, 2009). Members of the genus Thalassobacillus have been isolated from saline soil and hypersaline lake water (García et al, 2005;Sanchez-Porro et al, 2009).…”

“…Subsequently, a second species of the genus has been described, Thalassobacillus cyri (Sanchez-Porro et al, 2009). Members of the genus Thalassobacillus have been isolated from saline soil and hypersaline lake water (García et al, 2005;Sanchez-Porro et al, 2009). T. devorans was isolated from a phenol enrichment of saline soil samples and was found to degrade phenol (García et al, 2005).…”

“…T. devorans was isolated from a phenol enrichment of saline soil samples and was found to degrade phenol (García et al, 2005). The genus Thalassobacillus is characterized chemotaxonomically by having cell-wall peptidoglycan based on meso-diaminopimelic acid, MK-7 as the predominant menaquinone, anteiso-C 15 : 0 as the predominant fatty acid and a DNA G+C content of 42.4-43.0 mol% (García et al, 2005;Sanchez-Porro et al, 2009). Many novel bacterial strains have been isolated recently from the coast of Hwangdo (Hwang island) on the Yellow Sea, Korea (Jung et al, 2010).…”