Halioglobus maricola sp. nov., isolated from coastal seawater

Abstract

A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated IMCC14385T, was isolated from surface seawater of the East Sea, Republic of Korea. The 16S rRNA gene sequence analysis indicated that IMCC14385T represented a member of the genus Halioglobus sharing 94.6–97.8 % similarities with species of the genus. Whole-genome sequencing of IMCC14385T revealed a genome size of 4.3 Mbp and DNA G+C content of 56.7 mol%. The genome of IMCC14385T shared an average nucleotide identity of 76.6 % and digital DNA–DNA hybridization value of 21.6 % with the genome of Halioglobus japonicus KCTC 23429T. The genome encoded the complete poly-β-hydroxybutyrate biosynthesis pathway. The strain contained summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C17 : 1 ω8c as the predominant cellular fatty acids as well as ubiquinone-8 (Q-8) as the respiratory quinone. The polar lipids detected in the strain were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids, an unidentified aminolipid, an unidentified aminophospholipid and four unidentified lipids. On the basis of taxonomic data obtained in this study, it is suggested that IMCC14385T represents a novel species of the genus Halioglobus , for which the name Halioglobus maricola sp. nov. is proposed. The type strain is IMCC14385T (=KCTC 72520T=NBRC 114072T).

Metabolic Versatility of the FamilyHalieaceaeRevealed by the Genomics of Novel Cultured Isolates

“…nov. ( 14 ), and IMCC14385 as Halioglobus maricola sp. nov. ( 37 ) was reported using a polyphasic approach in our previous studies. JH123 formed a robust cluster with HIMB55, HTCC2080, and Luminiphilus syltensis NOR5-1B T in 16S rRNA gene- and genome-based trees.…”

“…In this study, we report the cultivation of six pure cultured Halieaceae strains and the characterization of their genomes. In addition to the two strains ( H. maricola IMCC14385 and A. fuscus IMCC3088) that were subjected to valid taxonomic descriptions in our previous studies ( 14 , 37 ), “ Ca. S. marina” IMCC8485 and “ Ca.…”

Sediminihaliea albiluteola gen. nov., sp. nov., a new member of the family Halieaceae, isolated from marine sediment

“…The family Halieaceae proposed by Spring et al [1], previously known as the OM60/NOR5 clade of the oligotrophic marine Gammaproteobacteria group [2], belongs to the order Cellvibrionales , class Gammaproteobacteria . The family Halieaceae includes 12 genera (https://lpsn.dsmz.de/family/halieaceae) at the time of writing, Haliea [3], Congregibacter [4], Chromatocurvus [5, 6], Halioglobus [7, 8], Luminiphilus [6], Pseudohaliea [6, 9], Parahaliea [10–12], Marimicrobium [13], Kineobactrum [14], Pseudohalioglobus [15, 16], Seongchinamella [15, 17] and ‘ Aequoribacter ’ (Li SH et al ., 2020 [18]). Most of them were isolated from marine environments, such as seawater, marine sediment, brine spring and tidal mudflat.…”

“…dsmz. de/ family/ halieaceae) at the time of writing, Haliea [3], Congregibacter [4], Chromatocurvus [5,6], Halioglobus [7,8], Luminiphilus [6], Pseudohaliea [6,9], Parahaliea [10][11][12], Marimicrobium [13], Kineobactrum [14], Pseudohalioglobus [15,16], Seongchinamella [15,17] and 'Aequoribacter' (Li SH et al, 2020 [18]). Most of them were isolated from marine environments, such as seawater, marine sediment, brine spring and tidal mudflat.…”

“…dsmz. de/ family/ halieaceae) at the time of writing, Haliea [3], Congregibacter [4], Chromatocurvus [5,6], Halioglobus [7,8], Luminiphilus [6], Pseudohaliea [6,9], Parahaliea [10][11][12], Marimicrobium [13], Kineobactrum [14], Pseudohalioglobus [15,16],…”