Hairy root type plant in vitro systems as sources of bioactive substances

Georgiev, Milen I.; Pavlov, Atanas; Bley, Thomas

doi:10.1007/s00253-007-0856-5

Cited by 310 publications

(169 citation statements)

References 81 publications

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“…Genetically transformed roots (so called hairy roots) mediated with Agrobacterium rhizogenes creates a rapid and simple tool to integrate and express foreign genes in plant cells (Georgiev et al 2007;Sharafi et al 2013aSharafi et al , 2014aHuet et al 2014). The molecular basis of hairy root induction is a transfer DNA (T-DNA) from root inducing (Ri) plasmid of A. rhizogenes.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Sharafi

Sohi

Mirzaee

et al. 2014

Physiol Mol Biol Plants

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In the present study, we developed an efficient protocol for in vitro plant regeneration and genetically transformed root induction in medicinal plant Artemisia aucheri Boiss. Leaf explants were cultivated in MS medium supplemented by combination of plant growth regulators including α-naphthalene-acetic acid, 6-benzyl-aminopurine, indole-3-acetic acid and 2, 4-dichlorophenoxyaceticacid. The highest frequency of shoot organogenesis occurred on MS medium supplemented with 0.05 mg/l NAA plus 2 mg/l BA (96.3 %) and MS medium supplemented with 0.5 mg/l IAA plus 2 mg/l BA (88.3 %). Root induction was obtained on MS medium supplemented with 0.5 mg/l IBA. This is a simple, reliable, rapid and high efficient regeneration system for A. aucheri Boiss in short period via adventitious shoot induction approach. Also, an efficient genetically transformed root induction for A. aucheri was developed through Agrobacterium rhizogenes-mediated transformation by four bacterial strains, A4, ATCC15834, MSU440, and A13 (MAFF-02-10266). The maximum frequency of hairy root induction was obtained using MSU440 (93 %) and ATCC15834 (89 %) bacterial strains. Hairy root lines were confirmed by PCR using the rolB gene specific primers and Southern blot analysis.

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Section: Introductionmentioning

confidence: 99%

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Sharafi

Sohi

Mirzaee

et al. 2014

Physiol Mol Biol Plants

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“…Hairy roots offer opportunities for stable (compared to undifferentiated cultures) and wide-range production of plant secondary metabolites (Giri and Narasu 2000;Hu and Du 2006;Georgiev et al 2007). They also present a potential for the production of valuable secondary metabolites by obtaining high-yield lines developed via optimization of the inoculums conditions and nutrient medium compounds, elicitation, permeabilization and cultivation of hairy roots in two phase systems, large scale cultivation in bioreactor systems, and using genetic engineering technology (Georgiev et al 2007).…”

Section: Introductionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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“…Hundreds of species have been transformed with A. rhizogenes with the aim of achieving transformed root induction. The prospects for the production of medicinal compounds in transformed roots were previously reviewed (Georgiev et al 2007;Guillon et al 2006;Uozumi 2004). Several Boraginaceous species have been also transformed with A. rhizogenes for the production of shikonin derivatives.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Sykłowska-Baranek

Pietrosiuk

Gawron

et al. 2011

Plant Cell Tiss Organ Cult

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The effects of medium exchange and methyl jasmonate addition on growth and production of shikonin and its derivatives acetylshikonin and isobutyrylshikonin in hairy root cultures of Lithospermum canescens were investigated. Responses varied depending on the transgenic line and stage of growth at which these lines were subjected to treatment. Shikonin itself was not detected, irrespective of the transgenic line and culture treatment used. A eightfold increase in acetylshikonin and isobutyrylshikonin accumulation was achieved when 32-day-old transgenic roots of Lc1D line were transferred from LS to M9 medium for the subsequent 3 weeks of culture. Methyl jasmonate exerted a detrimental effect on red naphthoquinones production. The extracts obtained from roots cultivated in M9 medium for 3 weeks were subjected to a cytotoxicity assay and displayed cytotoxic activity against human promyelocytic leukemia cells (HL-60) at the dose of 4 lg ml -1 .

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Hairy root type plant in vitro systems as sources of bioactive substances

Cited by 310 publications

References 81 publications

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

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