Hairy root induction in Oriental melon (Cucumis melo) by Agrobacterium rhizogenes and reproduction of the root-knot nematode (Meloidogyne incognita)

Abstract

Cotyledon explants of two Oriental melons produced hairy roots when cultured on Murashige and Skoog basal medium after infection by the Agrobacterium rhizogenes. Hairy roots were produced from the wounded surface of the cotyledon explants of Cucumis melo L. cv. Geumssaragi-euncheon on Murashige and Skoog selective medium and 86% of the GUS stained hairy roots were positive for the expression of beta-glucuronidase. The insertion of the gfp-gus fusion gene in the genomic DNA and the presence of the gfp-gus-specific transcript in the total RNAs of transgenic hairy roots were confirmed by PCR and RT-PCR, respectively. An immunoblot analysis of the transgenic hairy root extract revealed 97 kDa single bands coincident with the molecular weight of the GFP-GUS fusion proteins. ELISA demonstrated that the highest level of GFP-GUS fusion protein expression was 0.47% of the total soluble protein in a transgenic hairy root. The MS medium showed the fastest growth among three media types tested. Infection of the hairy roots with a root-knot nematode resulted in the development of a mature egg mass about 4-5 weeks after inoculation. The highest number of egg mass was obtained on the hairy roots cultured in SH medium containing 0.3% agar.

Evaluation of phenolic compounds, antioxidant and antimicrobial activities from transgenic hairy root cultures of gherkin ( Cucumis anguria L.)

“…It was revealed that hairy roots enhanced the amount of alkaloids in Lippia dulcis (Sauerwein et al, 1991), flavonoids in Daucus carota (BelRhlid et al, 1993), saponin in Bacopa monnieri (Majumdar et al, 2011), anthroquinones in Polygonum multiflorum (Thiruvengadam et al, 2014a), and polyphenols in Momordica charantia (Thiruvengadam et al, 2014b). Very few studies reported in the hairy root induction of Cucumis melo (Pak et al, 2009) and Cucumis sativus (Anuar et al, 2011). So far, there are no reports on the induction of hairy roots, and production of phenolic compounds and also their biological activities from hairy root cultures of C. anguria.…”

Composite Cucurbita pepo plants with transgenic roots as a tool to study root development

“…Previous studies reported success in establishing hairy roots for Cucumis sativus, Cucumis melo and Cucurbita pepo (Katavić et al, 1991;Sanita di Toppi et al, 1997;Pak et al, 2009;Anuar et al, 2011), based on the infection of excised cotyledons. However, so far no protocol has been established for the production of composite transgenic plants of squash.…”

Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of <em>Lavandula angustifolia</em>

“…3, a-h). Nutrition-dependent growth of hairy roots was also reported in some plant species such as Cucumis melo (Pak et al, 2009), Arachis hypogaea (Condori et al, 2010), and Momordica charantia (Thiruvengadam et al, 2014). Both types of auxins initially stimulated the growth of hairy roots, but after two months a different trend was noticed in IAA-treated hairy roots as they stopped growing and their color gradually turned dark brown.…”