Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of <em>Lavandula angustifolia</em>

Abstract

Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA of a root-inducing plasmid, regulate auxin sensitivity of hairy roots. Therefore, this study was aimed to improve the growth and rosmarinic acid production of Lavandula angustifolia hairy roots. Lateral branches of hairy roots were transferred to ½ MS and ½ B5 liquid media. To assess auxin sensitivity, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) with four different concentrations (0, 0.1, 0.5 and 1 mg/l) were also applied. The growth of hairy roots in ½ MS medium was two-fold higher than in ½ B5 medium. In addition, both auxins were found to significantly improve the growth of hairy roots whereas non-transformed roots stopped growing in the presence of the auxins. The highest dry weight and rosmarinic acid production of hairy roots were obtained from ½ MS medium supplemented with IBA irrespective of its concentration. As a result, the hairy roots grown in ½ MS medium supplemented with IBA produced the maximum amount of rosmarinic acid (7.98 mg/g dry weight of hairy roots). This first report of rosmarinic acid production in L. angustifolia hairy roots provides new insights into the auxin sensitivity of L. angustifolia hairy roots.

Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia

“…In addition, the accumulation of rosmarinic acid increased with an increase in the IAA concentration (0-1 mg/L). On the other hand, rosmarinic acid production is reduced in Lavandula angustifolia hairy roots when the IBA concentration increases [27]. Specifically, rosmarinic acid content in hairy roots grown with 1 mg/L IBA (0.58 mg/g DW)…”