Hairy Cell Leukaemia: Seven Cases with Probable B‐Lymphocytic Origin

Abstract

Mononuclear cells from seven patients with hairy cells leukaemia were examined for features suggestive of either a lymphocytic or monocytic origin. Immunofluorescent staining of both methanol fixed and incubated cells, using monospecific antisera, revealed a predominant cell-associated immunoglobulin in each case. Three were positive for mu and kappa chains, two for gamma and kappa chains, one for delta and kappa chain determinants and one reacted only with antigamma chain serum. Formation of EAC rosettes, a feature of both B lymphocytes and monocytes, was variable. T cells, as judged by E rosettes, were not elevated in any patient. Phytohaemagglutinin reactivity was normal in six and depressed in one case. With the exception of minimal activity in assays for glass adherence and latex particle phagocytosis, none of the cells showed features typical of monocytes. Hairy cells were negative by peroxidase stain and lacked the electron microscopic characteristics of monocytes. They did not react in either rosette or phagocytic assays with anti-A or anti-D coated erythrocytes nor did they elaborate granulocyte colony stimulating factor, a monocyte-derived in vitro granulopoietin. Although unequivocal classification of these abnormal cells is not possible, the data storngly suggests that this represents a variant of a B lymphocytic neoplasm.

Hairy‐Cell Leukaemia: a B‐Lymphocytic Disorder

“…The presence of C3 receptors on H C is much more controversial. Some workers did not find such a receptor (Burns et al, 1977b; this study), whereas others did (Jaffe et al, 1974;Boldt et al, 1977;Utsinger et al, 1977;Zidar et al, 1977;Braylan et al, 1978). It has been supposed by Burns et a2 (1977a, b) that this EAIgMC-rosetting may be due to the presence of a receptor for the Fc part of IgM (FcIgM), which is demonstrable with EAIgM-rosettes.…”

“…So far, the cytoplasmic-fluorescence method has been applied less often than the surfacefluorescence method. Seven cases with , u (Golde et al, 1977;Zidar et al, 1977; this study), one case with 6 (Zidar et all 1977) The demonstration of membrane receptors on a given cell depends on the sensitivity of the test system and the specificity of the reagents. The overwhelming diversity of the techniques used in the studies reported in the literature, makes it difficult to compare the results obtained in HCL.…”

“…This is also possible by preincubation of the cells with aggregated IgG (Leech et al, 1975). Furthermore, cells release exogenous Ig during overnight incubation (Zidar et al, 1977;Braylan et al, 1978) and, finally, the cells can be trypsinized and the reappearance of surface Ig can be followed (Leech et al, 1975 We found that mild fixation with diluted formaldehyde appears to be an attractive alternative for these procedures. This procedure has been used in our laboratory for the last 2 years.…”

Therapieergebnisse bei Hairy-cell-Leukämie

“…Trotz einiger Gemeinsamkeiten ist die Leukämie abzugren zen von anderen lymphatischen und monozytären Hämobla-stosen. Einige gemeinsame Merkmale mit speziellen lympha tischen Subpopulationen oder mit Monozyten werden aber als Grundlage für eine Zuordnung diskutiert [5,6,7,8,21,22]. Es ist dennoch fraglich, ob die Behandlung an bekannte Ver fahren angelehnt werden kann.…”

Surface immunoglobulin, lectin-induced cap formation and phagocytic function in five patients with the leukemic phase of hairy cell leukemia

“…This observation was subsequently supported by Utsinger et a1.I7 in 3 patients whose hairy cells phagocytosed latex, as well as by Zidar er a1. 18 Our laboratory found that there was a spectrum of functional characteristics when 14 patients were studied; appproximately 50% of the patients had hairy cells that phagocytosed zymosan, whereas the others did not." Naeim et a1.…”