Hair Growth Promoting Effects of 650 nm Red Light Stimulation on Human Hair Follicles and Study of Its Mechanisms via RNA Sequencing Transcriptome Analysis

Yang, Kai; Tang, Yulong; Ma, Yanyun; Liu, Qingmei; Huang, Yan; Zhang, Yuting; Shi, Xiaofeng; Zhang, Li; Zhang, Yue; Wang, Ji'an; Zhu, Yifei; Liu, Wei; Tan, Yimei; Lin, Jinran; Wu, Wenyu

doi:10.5021/ad.2021.33.6.553

Cited by 15 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also observed that the volunteers in the group treated with blue light, in addition to improvements in density, also experienced a decrease in scalp erythema and seborrhea, as well as an increase in hair color, findings consistent with those reported by Buscone et al [6]. Given the benefits found in the results of this study regarding the use of red and blue light in the treatment of Androgenetic Alopecia, further studies are encouraged to evaluate the combined use of the two wavelengths.…”

Section: A B a B A Bsupporting

confidence: 92%

“…Blue light, with wavelengths ranging from 415 nm to 495 nm, has been pointed out as a potential treatment for AGA by researchers who identified photoreceptors in the hair follicle such as OPNS2 and OPSN3 [6] and Cryptochrome 1 [7], enabling the absorption of this wavelength to promote reactions of mesenchymal cells in the dermal papilla, as well as anti-inflammatory effects. The aim of this work was to evaluate the effects of phototherapy in the treatment of AGA, using two different wavelengths: red and blue.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The use of phototherapy with different wavelengths in the treatment of Androgenetic Alopecia

Da Silva,

Baptista

2024

Braz J Hair Health

View full text Add to dashboard Cite

Introduction: Phototherapy has been identified as a safe, non-invasive, and promising resource for the treatment of Androgenetic Alopecia (AGA). The aim of this study was to evaluate the effects of phototherapy on the treatment of AGA using two different wavelengths: red and blue. Both red and blue light, under the parameters tested in this study, allowed an increase in hair density, a reduction in the central parting, and promoted an increase in the number of terminal hairs after the treatments, as well as a reduction in vellus hairs. Methodology: Ten volunteers were selected (GLV n=5) and (GLA N=5), all diagnosed with AGA and approved by the ethics committee with opinion number 5,761,579. Interventions were carried out using Vênus equipment (MMOptics, São Carlos, Brazil) with Group GLV λ=660nm, 4J and Group GLA λ=440 nm, 4J. The interventions were performed once a week, for 90 days, totaling 12 treatment sessions. Hair progression was assessed by photographic record and the ratio of vellus to terminal hairs was performed by digital trichoscopy imaging (Dino Lite; New Taipei City, Taiwan, China). Results and Conclusion: Both red and blue light, under the parameters tested in this study, allowed an increase in hair density, a reduction in the central parting, and promoted an increase in the number of terminal hairs after the treatments, as well as a reduction in vellus hairs.

show abstract

Section: A B a B A Bsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The use of phototherapy with different wavelengths in the treatment of Androgenetic Alopecia

Da Silva,

Baptista

2024

Braz J Hair Health

View full text Add to dashboard Cite

show abstract

“…Temperature and light can also affect the growth of hair follicles. Studies have shown that red light at 650 nm can promote the proliferation of human hair follicle cells and significantly delay the transition of hair follicles from anagen to telogen ( Yang et al, 2021 ). The influence of gender factors on hair growth cycle is mainly regulated by hormones, and androgen has a high influence on hair growth and cycle ( Grymowicz et al, 2020 ).…”

Section: Hair Follicle Cyclingmentioning

confidence: 99%

Morphogenesis, Growth Cycle and Molecular Regulation of Hair Follicles

Lin

Liang

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

As one of the main appendages of skin, hair follicles play an important role in the process of skin regeneration. Hair follicle is a tiny organ formed by the interaction between epidermis and dermis, which has complex and fine structure and periodic growth characteristics. The hair growth cycle is divided into three continuous stages, growth (anagen), apoptosis-driven regression (catagen) and relative quiescence (telogen). And The Morphogenesis and cycle of hair follicles are regulated by a variety of signal pathways. When the signal molecules in the pathways are abnormal, it will affect the development and cycle of hair follicles, which will lead to hair follicle-related diseases.This article will review the structure, development, cycle and molecular regulation of hair follicles, in order to provide new ideas for solving diseases and forming functional hair follicle.

show abstract

“…Recently, low‐level laser (or light) therapy (LLLT) for the treatment of androgenetic alopecia (AGA) has been approved by the FDA. LLLT promotes hair growth using red light, which stimulates cell metabolism, shifts follicles into anagen, and prolongs the anagen phase 4–6 . LLLT appears to be a safe and effective treatment in AGA, 7–9 while a standardized protocol for LLLT in AGA is yet unavailable due to the diversity of various devices and methods.…”

Section: Introductionmentioning

confidence: 99%

“…LLLT promotes hair growth using red light, which stimulates cell metabolism, shifts follicles into anagen, and prolongs the anagen phase. [4][5][6] LLLT appears to be a safe and effective treatment in AGA, [7][8][9] while a standardized protocol for LLLT in AGA is yet unavailable due to the diversity of various devices and methods. It was reported that variations in gender, age, and skin conditions showed different responses to treatment, and the absorption of the laser light is impeded by the existing hairs.…”

Section: Introductionmentioning

confidence: 99%

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Hasegawa

Fujimoto²,

Mita

et al. 2022

Lasers Surg Med

View full text Add to dashboard Cite

Objectives Hair loss, including alopecia, is a common dermatological issue worldwide. At present, the application of fractional carbon dioxide (CO2) laser in the treatment of alopecia has been documented; however, the results vary between reports. These varying results may be due to the limited knowledge of cellular action in laser‐irradiated skin. The objective of this study was to investigate the molecular and cellular mechanisms of laser treatment under effective conditions for hair cycle initiation. Methods A fractional CO2 laser was applied and optimized to initiate the hair cycle in a mouse model of alopecia. Several cellular markers were analyzed in the irradiated skin using immunofluorescence staining. Cellular populations and their comprehensive gene expression were analyzed using single‐cell RNA sequencing and bioinformatics. Results The effective irradiation condition for initiating the hair cycle was found to be 15 mJ energy/spot, which generates approximately 500 μm depth columns, but does not penetrate the dermis, only reaching approximately 1 spot/mm2. The proportion of macrophage clusters significantly increased upon irradiation, whereas the proportion of fibroblast clusters decreased. The macrophages strongly expressed C–C chemokine receptor type 2 (Ccr2), which is known to be a key signal for injury‐induced hair growth. Conclusions We found that fractional CO2 laser irradiation recruited Ccr2 positive macrophages, and induced hair regrowth in a mouse alopecia model. These findings may contribute to the development of stable and effective fractional laser irradiation conditions for human alopecia treatment.

show abstract

Hair Growth Promoting Effects of 650 nm Red Light Stimulation on Human Hair Follicles and Study of Its Mechanisms via RNA Sequencing Transcriptome Analysis

Cited by 15 publications

References 30 publications

The use of phototherapy with different wavelengths in the treatment of Androgenetic Alopecia

The use of phototherapy with different wavelengths in the treatment of Androgenetic Alopecia

Morphogenesis, Growth Cycle and Molecular Regulation of Hair Follicles

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia

Contact Info

Product

Resources

About

Hair Growth Promoting Effects of 650 nm Red Light Stimulation on Human Hair Follicles and Study of Its Mechanisms via RNA Sequencing Transcriptome Analysis

Cited by 15 publications

References 30 publications

The use of phototherapy with different wavelengths in the treatment of Androgenetic Alopecia

The use of phototherapy with different wavelengths in the treatment of Androgenetic Alopecia

Morphogenesis, Growth Cycle and Molecular Regulation of Hair Follicles

Single‐cell transcriptome analysis of fractional CO2 laser efficiency in treating a mouse model of alopecia

Contact Info

Product

Resources

About

Single‐cell transcriptome analysis of fractional CO₂ laser efficiency in treating a mouse model of alopecia