Hair Follicle Morphogenesis in the Treatment of Mouse Full‐Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells

Wu, Minjuan; Xiong, Jie; Shiyun, Shao; Xu, Shaochun; Ni, Haitao; Wang, Yue; Ji, Kaihong

doi:10.1155/2016/8281235

Cited by 17 publications

(16 citation statements)

References 18 publications

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“…In recent years, it has been clarified that ASCs could activate fibroblasts by secreted cytokines for skin aging treatment. Through differentiation, type I, IV and V collagens were stimulated, and fibroblasts were reorganized to increase the matrix composition of skin cell, so as to achieve the effect of dermal tissue and skin structure repair [11,12]. In this study, we observed the effect of autologous ASCs on the thickness of dermis and the number of fibroblasts by constructing nude mice photoaging group.…”

Section: Discussionmentioning

confidence: 94%

Effects of autologous adipose stem cells on dermis layer thickness and fibroblast cells number in photoaging nude mice model

Yu¹,

Yuan²,

Liu³

2018

biomedicalresearch

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Objective: To investigate the effects of autologous Adipose Stem Cells (ASCs) on the thickness of dermis and the number of fibroblasts in nude mice. As a result, relevant experimental basis was provided for clinical application of autologous ASCs in skin photoaging therapy. Methods: 50 SPF grade balb/c nude mice were selected and divided into 5 groups: normal group (n=10), photoaging model group (n=10), photoaging+ASCs group (n=10), photoaging+PBS group (n=10), photoaging+aminoguanidine group (n=10). In addition to the normal group, the remaining groups were constructed by UV irradiation to establish a photoaging model. After successful modeling, the mice in the ASCs group were injected with 1 ml ASCs (at a concentration of 1 × 10 6 cells/ml); the nude mice in the photoaging+PBS group were performed intradermal injection of equal dose of PBS. The mice in the photoaging+aminoguanidine group were performed intradermal injection of aminoguanidine (100 mg/ kg). After 4 w of feeding, dorsal skin in each group was observed. The thickness of the dermis was observed by Masson staining, and the number of fibroblasts was counted. Result: The skin gloss and elasticity of the nude mice were significantly lower than those in the normal group. The skin gloss and elasticity of photoaging+ASCs group and photoaging+aminoguanidine group were significantly higher than those of the normal group. Compared with the normal group, the dermal thickness and the number of fibroblasts decreased in photoaging group (P<0.05), which accorded with the characteristics of photoaging; the dermal thickness and the number of fibroblasts were significantly increased (P<0.05) in the photoaging+ASCs group and photoaging+aminoguanidine group. There was no significant difference in the number of dermal thickness and fibroblasts between the PBS group and the model group (P>0.05). Conclusion: ASCs transplantation can significantly increase the dermal layer thickness of nude mice and increase the number of fibroblasts. ASCs transplantation can be used to improve skin aging caused by light injury.

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Section: Discussionmentioning

confidence: 94%

Effects of autologous adipose stem cells on dermis layer thickness and fibroblast cells number in photoaging nude mice model

Yu¹,

Yuan²,

Liu³

2018

biomedicalresearch

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“…Subsequently, hADSCs or an equal volume of DMEM were injected around the wounds, and it was observed that wounds with the hADSC injection had a better closure efficiency. Finally, tissue sections were used to further observe the morphological changes in wound repair [20]. It was also confirmed that seawater immersion hindered the morphological repair of wounds, and hADSCs reduced the impact of seawater on wounds and promoted their repair.…”

Section: The Effect Of Hadscs On the Treatment Of Seawater-mentioning

confidence: 84%

“…Characterization of hADSCs. According to previous research methods, hADSCs were extracted, and the osteogenic and adipogenic differentiation potentials were analyzed [14,20]. Primary hADSCs began to adhere to wells, and cell morphology was typical after 3 to 7 days of culture, as shown in Figure 3(a).…”

Section: Resultsmentioning

confidence: 99%

“…Wounds after hADSC Treatment. Previous studies have shown that Ki67 is an important nuclear protein for cell proliferation, and CK19 is an important membrane protein for skin stem cell proliferation [16,20,23]. The proliferation of cells (especially skin stem cells) plays an important role in the repair of skin wounds.…”

Section: Ki67 and Ck19 Expression Levels In Seawater-immersedmentioning

confidence: 99%

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Human Adipose-Derived Stem Cells Promote Seawater-Immersed Wound Healing by Activating Skin Stem Cells via the EGFR/MEK/ERK Pathway

Xiong

Wang

et al. 2019

Stem Cells International

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Seawater (SW) immersion can increase the damage of skin wounds and produce refractory wounds. However, few studies have been conducted to investigate the mechanisms of SW immersion on skin wounds. In our current study, we investigated the effect of human adipose-derived stem cells (hADSCs) on the repair of SW-treated full-thickness skin wounds and the underlying mechanisms. The results showed that SW immersion could reduce the expression of EGF and suppress the activation of the MEK/ERK signaling pathway. At the same time, the proliferation and migration of skin stem cells were inhibited by SW immersion, resulting in delayed wound healing. However, hADSCs significantly accelerated the healing of SW-immersed skin wounds by promoting cell proliferation and migration through the aforementioned mechanisms. Our results indicate a role for hADSCs in the repair of seawater-immersed skin wounds and suggest a potential novel treatment strategy for seawater-immersed wound healing.

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“…HFTs are adult stem cells and have a marked proliferation ability in the skin wound healing process (12,13). An immunohistochemical study suggested that HFTs are able to induce hair follicle growth by targeting Wnt10b (14).…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway

Cai

Zheng

et al. 2018

Mol Med Report

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Long non-coding RNAs (lncRNAs) are defined as non-coding transcripts (>200 nucleotides) that serve important roles in the proliferation and differentiation of stem cells. Hair follicle stem cells (HFTs) have multidirectional differentiation potential and are able to differentiate into skin, hair follicles and sebaceous glands, serving a role in skin wound healing. The aim of the present study was to analyze the regulatory role of lncRNA AK015322 (IncRNA5322) in HFTs and the potential mechanism of IncRNA5322‑mediated differentiation of HFTs. The results demonstrated that lncRNA5322 transfection promoted proliferation and differentiation in HFTs. It was identified that lncRNA5322 transfection upregulated the expression and phosphorylation of phosphoinositide 3‑kinase (PI3K) and protein kinase B (AKT) in HFTs. It was also observed that lncRNA5322 transfection upregulated microRNA (miR)‑21 and miR‑21 agonist (agomir‑21) eliminated lncRNA5322‑induced expression and phosphorylation of PI3K and AKT. The present study also demonstrated that agomir‑21 blocked IncRNA5322‑induced expression and phosphorylation of PI3K and AKT in HFTs. The results indicated that agomir‑21 transfection also suppressed the IncRNA5322‑induced proliferation and differentiation of HFTs. In conclusion, the results of the present study suggest that lncRNA5322 is able to promote the proliferation and differentiation of HFTs by targeting the miR‑21‑mediated PI3K‑AKT signaling pathway in HFTs.

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Hair Follicle Morphogenesis in the Treatment of Mouse Full‐Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells

Cited by 17 publications

References 18 publications

Effects of autologous adipose stem cells on dermis layer thickness and fibroblast cells number in photoaging nude mice model

Effects of autologous adipose stem cells on dermis layer thickness and fibroblast cells number in photoaging nude mice model

Human Adipose-Derived Stem Cells Promote Seawater-Immersed Wound Healing by Activating Skin Stem Cells via the EGFR/MEK/ERK Pathway

Long non‑coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway

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