Haemorrhagic disease in cattle with genotype 1 bovine viral diarrhoea virus infection

Colloff, Adrian; Watson, Phil; Duff, J. Paul; Scholes, S. F. E.

doi:10.1136/vr.100497

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…Nucleated RBCs were seen on the blood film indicating a regenerative anaemia and histology showed the bone marrow was reactive. Haemorrhagic disease in BVDV1 infection in cattle is associated with thrombocytopenia (Colloff et al., 2012) and that may be the explanation in this pig.…”

Section: Discussionmentioning

confidence: 82%

Investigation into an outbreak of Border disease virus in pigs in England

Dastjerdi

Strong

Rocca

et al. 2022

Transbounding Emerging Dis

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Border disease (BD) was first reported in 1959 in lambs from the border region of England and Wales. The causative virus (BD virus; BDV) has since been identified in several other ruminant species and pigs. The virus is prevalent in sheep flocks of UK, Europe and USA and has potential to inflict substantial economic losses. Natural BDV infection of pigs was first reported in the UK in 1992 from pigs with haemorrhagic lesions and more recently from healthy pigs in Spain and Japan. Here, a persistent problem of poor growth and anaemia in a small proportion of growing pigs on a mixed pig and sheep holding was investigated and tissues were tested in a pan viral microarray.The microarray detected BDV RNA in several tissues which was further confirmed by sequencing, specific BDV PCR and immunohistochemistry. Phylogenetically, the virus clustered with other BDVs in the sub-genotype 1b. This investigation highlights likely interspecies transmission of pestiviruses and their impact on pestivirus detection and eradication programs.

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Section: Discussionmentioning

confidence: 82%

Investigation into an outbreak of Border disease virus in pigs in England

Dastjerdi

Strong

Rocca

et al. 2022

Transbounding Emerging Dis

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“…BVDV is ubiquitous and causes a range of clinical manifestations (including abortion, respiratory problems, chronic wasting disease, immune system dysfunction, and predisposition to secondary viral and bacterial infections). BVDV-1 and -2 strains can cause acute fatal disease with mortality rates of 17-32% [4][5][6]. BVDV is also able to establish a persistent infection in fetuses [7].…”

Section: Introductionmentioning

confidence: 99%

Quinolinecarboxamides Inhibit the Replication of the Bovine Viral Diarrhea Virus by Targeting a Hot Spot for the Inhibition of Pestivirus Replication in the RNA-Dependent RNA Polymerase

et al. 2020

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The bovine viral diarrhea virus (BVDV), a pestivirus from the family of Flaviviridae is ubiquitous and causes a range of clinical manifestations in livestock, mainly cattle. Two quinolinecarboxamide analogues were identified in a CPE-based screening effort, as selective inhibitors of the in vitro bovine viral diarrhea virus (BVDV) replication, i.e., TO505-6180/CSFCI (average EC50 = 0.07 µM, SD = 0.02 µM, CC50 > 100 µM) and TO502-2403/CSFCII (average EC50 = 0.2 µM, SD = 0.06 µM, CC50 > 100 µM). The initial antiviral activity observed for both hits against BVDV was corroborated by measuring the inhibitory effect on viral RNA synthesis and the production of infectious virus. Modification of the substituents on the quinolinecarboxamide scaffold resulted in analogues that proved about 7-fold more potent (average EC50 = 0.03 with a SD = 0.01 µM) and that were devoid of cellular toxicity, for the concentration range tested (SI = 3333). CSFCII resistant BVDV variants were selected and were found to carry the F224P mutation in the viral RNA-dependent RNA polymerase (RdRp), whereas CSFCI resistant BVDV carried two mutations in the same region of the RdRp, i.e., N264D and F224Y. Likewise, molecular modeling revealed that F224P/Y and N264D are located in a small cavity near the fingertip domain of the pestivirus polymerase. CSFC-resistant BVDV proved to be cross-resistant to earlier reported pestivirus inhibitors (BPIP, AG110, LZ37, and BBP) that are known to target the same region of the RdRp. CSFC analogues did not inhibit the in vitro activity of recombinant BVDV RdRp but inhibited the activity of BVDV replication complexes (RCs). CSFC analogues likely interact with the fingertip of the pestivirus RdRp at the same position as BPIP, AG110, LZ37, and BBP. This indicates that this region is a “hot spot” for the inhibition of pestivirus replication.

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“…12 However, there have been reports of BVDV-1 and, more specifically, the BVDV-1b subtype isolated from cattle with hemorrhage. 1,5,14,16,23 The occurrence of BVDV infections with hemorrhages is possible in fetuses.…”

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confidence: 99%

Bovine viral diarrhea virus 1b fetal infection with extensive hemorrhage

et al. 2017

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Bovine viral diarrhea virus (BVDV) 1b was isolated from tissues of a term bovine fetus with petechial hemorrhages noted throughout the body and placenta at autopsy. Fresh lung, kidney, thymus, and liver tissues were examined by direct fluorescent antibody testing and were positive for BVDV antigen and negative for bovine herpesvirus 1 antigen. An organ pool of fresh tissues was positive for noncytopathic (NCP) BVDV-1 by virus isolation. BVDV-1b was identified by sequencing of the 5'-UTR region of the genome. Fixed brain, placenta, thymus, lymph node, lung, kidney, skeletal muscle, liver, and bone marrow were positive for BVDV antigen by immunohistochemistry. Although BVDV hemorrhage and/or thrombocytopenia has been associated historically with NCP strains of BVDV-2, this case adds to more recent reports of BVDV-1 infections and hemorrhage in cattle. This BVDV-1b isolate should be investigated for its potential to cause hemorrhage in postnatal cattle.

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Haemorrhagic disease in cattle with genotype 1 bovine viral diarrhoea virus infection

Cited by 7 publications

References 16 publications

Investigation into an outbreak of Border disease virus in pigs in England

Investigation into an outbreak of Border disease virus in pigs in England

Quinolinecarboxamides Inhibit the Replication of the Bovine Viral Diarrhea Virus by Targeting a Hot Spot for the Inhibition of Pestivirus Replication in the RNA-Dependent RNA Polymerase

Bovine viral diarrhea virus 1b fetal infection with extensive hemorrhage

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